DOI QR코드

DOI QR Code

Experiments on Avoiding and Deodorizing and Antibacterial of Haemaphysalis longicornis (Ixodoidea: Ixodidae) of Complex Extracts Including Water-Soluble Phytoncide

수용성 피톤치드(water-soluble phytoncide)를 포함한 복합추출물의 작은소피참진드기(Haemaphysalis longicorins) 기피와 탈취 및 항균 실험

  • Seul Gi Kwon (Supio Co., Ltd.) ;
  • Hyo Jung Jung (Supio Co., Ltd.) ;
  • Jun Young Jung (Department of Pharmaceutical Engineering, College of Medical & Life Science, Silla University) ;
  • and Jae Hwa Lee (Department of Pharmaceutical Engineering, College of Medical & Life Science, Silla University)
  • 권슬기 ((주)수피오) ;
  • 정효정 ((주)수피오) ;
  • 정준영 (신라대학교 의생명과학대학 제약공학과) ;
  • 이재화 (신라대학교 의생명과학대학 제약공학과)
  • Received : 2022.12.13
  • Accepted : 2023.05.26
  • Published : 2023.06.01

Abstract

The number of households with pets has increased in recent years. Consequently, interest in mites and their odors has increased. In this study, we investigated whether pyrethrum extract and water-soluble phytoncides are effective tick repellents, deodorizing agents, and antibacterial agents. In the tick-climbing test, the pyrethrum extract (23.33 points) and water-soluble phytoncide (22.00 points) showed a high repelling effect. To confirm the tick avoidance effect of the composite extract, a tick climbing test was conducted with a pest control extract, a composite extract (referred to as a sample) containing a water-soluble phytoncide, and a third-party product (control). The sample showed a high avoidance effect (13.00 points and Control 26.67 points. An indoor repellent efficacy test for ticks confirmed that the sample showed an excellent tick-repellent effect (88%) compared to the control (12%). As a result of a deodorization test, ammonia was shown to have a deodorizing effect of 98.3% and trimethylamine of 99.5%, whereas, in the antibacterial test, the E. coli, Staphylococcus aureus, and pneumonia groups showed an antibacterial effect of 99.9%. Combining the results of this study, it is judged that the complex extract including the above material has high utility value as a material that is effective in mite repellents, deodorization, and antibacterial activity.

최근 들어 반려동물을 키우는 가구 수가 증가하고 있다. 그로인해 진드기와 냄새에 대한 관심 또한 증가하고 있다. 본 연구는 제충국 추출물(Tanacetum cinerariifolium), 수용성 피톤치드(water-soluble phytoncide)가 진드기 퇴치제로서 효과와 탈취 및 항균 효과가 있는지 알아보기 위하여 연구를 진행하였다. 틱 클라이밍 테스트(Tick climbing test)에서 제충국 추출물(Tanacetum cinerariifolium) 23.33점, 수용성 피톤치드 22.00점으로 높은 기피효과를 보였다. 복합추출물로서의 진드기 기피 효과를 확인하기 위해 위 실험에서 기피 효과가 확인된 제충국 추출물, 수용성 피톤치드를 포함한 복합추출물(Sample이라 함.), 타사 제품(Control이라 함.)로 틱 클라이밍 테스트(Tick climbing test)를 진행하였다. 결과 Sample 13.00점, Control 26.67점으로 Sample이 높은 기피 효과를 나타내었다. 참진드기 실내 기피제 효력 시험법에서도 Sample이 88%, Control이 12%로 우수한 진드기 기피효과를 보인다는 것을 확인하였다. Sample을 가지고 탈취 시험을 진행한 결과 암모니아에서 98.3%, 트리메틸아민에서 99.5%의 탈취 효과를 보여주었으며, 항균시험에서 대장균, 황색포도상구균, 폐렴균 모두 99.9%의 항균 효과를 보여주었다. 본 연구결과를 종합해 볼 때 위 소재를 포함한 복합추출물은 추후 진드기 기피제와 탈취 및 항균에도 효과가 있는 소재로서의 활용 가치가 높다고 판단된다.

Keywords

Acknowledgement

본 연구는 부산광역시와 신라대학교 대학혁신연구단지조성사업의 기술개발사업으로 수행된 연구결과입니다.

References

  1. Anderson, J.F., Magnarelli, L.A., 2008. Biology of ticks. Infectious Disease Clinics of North America 22, 195-215. https://doi.org/10.1016/j.idc.2007.12.006
  2. Baptista, J., Blache, D., Cox-Witton, K., Craddock, N., Dalziel, T., de Graaff, N., Fernandes, J., Green, R., Jenkins, H., Kahn, S., Kelly, D., Lauber, M., K. Maloney , S., Peachey, B., Rodger, I., Skuse, J., Tilbrook, A.J., Rohan Walker, F., Wall, K., Zito, S., 2021. Impact of the COVID-19 pandemic on the welfare of animals in Australia. Front. Vet. Sci. 7, 621843.
  3. Boulanger, N., Boyer, P., Talagrand-Reboul, E., Hansmann, Y., 2019. Ticks and tick-borne diseases. MMI Formation 49, 87-97. https://doi.org/10.1016/j.medmal.2019.01.007
  4. Chae, J.-B., Kim, T.-H., Jung, J.-H., Park, Y.-J., Park, J.-H., Choi, K.-S., Yu, D.-H., Chae, J.-S., 2017. Prevalence of severe fever with thrombocytopenia syndrome virus among ticks surveyed at Mt. Gwanak, Korea. Korean J. Vet. Res. 57, 169-174. https://doi.org/10.14405/kjvr.2017.57.3.169
  5. Chae, J.S., 2017. Survey of severe fever with thrombocytopenia syndrome virus in natural environment and study on useful meterial for development of tick repellent. Seoul National University Industry-University Cooperation Foundation. National Institute of Environmental Sciences. pp. 94-145.
  6. Chauvin, R.C., 1946. Sur la substance guin dans less faulles de Melia azedirach responseless criquez. Acad. Sci. Paris. 222, 41-414.
  7. Chen, Y.L., Casida. J.E., 1969. Photodecomposition of pyrethrin I, allethrin, phthalthrin, and dimethrin. Modifications in the acid moiety. J. Agr. Food. Chem. 17, 208-215. https://doi.org/10.1021/jf60162a036
  8. Crombie, L., 1995. Chemistry of pyrethrins. in: Casida, J.E., Quistad, G.B. (Eds.), Pyrethrum flowers: production, chemistry, toxicology and uses. Oxford University Press, New York, pp. 108-122.
  9. Davies, M.H., Soto, R.J., Stewart, R.D., 1988. Toxicity of diethyltoluamide-containing insect repellents. JAMA 259, 2239.
  10. Ellenhorn, M., 1997. Ellenhorn'smedical toxicology: diagnosis and treatment of human poisoning. 2nd ed. William & Wilkins, Baltimore.
  11. Endenburg, N., Knol, B.W., 1994. Behavioural, household, and social problems associated with companion animals: opinions of owners and non-owners. Vet. Quart. 16, 130-134. https://doi.org/10.1080/01652176.1994.9694434
  12. Fantahun, B., Mohamed, A., 2012. Survey on the distribution of tick species in and around Assosa Town, Ethiopia. J. Vet. Sci. 5, 32-41. https://doi.org/10.3923/rjvs.2012.32.41
  13. Han, S.-Y., Sung, S.-H., Seo, J.-W., Kim, J.H., Lee, S.-J., Yoo, S.-S., 2021. Isolation and identification of tick-borne pathogens in hard ticks collected in Daejeon. Korean J. Vet. Serv. 44, 93-102.
  14. Head, S.W., 1966. A study of the insecticidal constituents in Chrysanthemum cinerarizefolium. Pyrethrum Post. 8, 32-37.
  15. Hiramatsu, Y., Miyazaki, Y., 2001. Effect of volatile matter from wood chips on the activity of house dust mites and on the sensory evaluation of humans. J. Wood. Sci. 47, 13-17. https://doi.org/10.1007/BF00776639
  16. Jang, G.S., 2017. Selection of natural repellent agents from plant for management of tick. Korea Center for Diease Control and Prevention. Ministry of Health & Welfare (MW)(MW).
  17. Jung, N.S., Park, S.N., 2021. 2020 Population and housing census sample count results population household basic items. https://kostat.go.kr/portal/korea/kor_nw/1/1/index.board?bmode=read&aSeq=403144 (accessed on 11 June, 2022).
  18. Jung, S.C., 2010. Climate change and Zoonosis. J. Food Hyg. Saf. 5, 25-32.
  19. Kang, H.Y., Lee, S,Y., Choi, I.K., 1993. The antifungal activity of coniferous needle oil. J. Kor. For. En. 13, 71-77.
  20. Kim, B.Y., Jo, J.Y., Choi, D.Y., Kang, G.H., Kim, A.R., 2021. 2021 Animal protection welfare public awareness survey. Ministry of Agriculture, Forestry and Fisheries Food Education and Culture, p. 12.
  21. Kim, M.Y., Park, S.K., Park, S.H., 2020. Center for disease control. Zoological infectious disease control division, medical staff are investigating the outbreak of severe fever with thrombocytopeniasyndrome (SFTS). https://www.kdca.go.kr/board/board.es?mid=a20501010000&bid=0015&act=view&list_no=368102 (accessed on 10 June, 2022).
  22. Kim, S.Y., Park, H.I., 2018. The Effect of pet on Happinessthrough support-seeking emotion regulation: a mediation model moderated by mindfulness. KJHP 23, 1001-1018. https://doi.org/10.17315/kjhp.2018.23.4.010
  23. Lee, S.H., Chai, J.Y., Kho, W.G., Hong, S.J., Chung, Y.D., 1989. A human case of tick bite by Ixodes nipponensis on the scalp. Korean J. Parasitol. 27, 67-69. https://doi.org/10.3347/kjp.1989.27.1.67
  24. Moon, J.S., 2010. Effect of climate change on animal diseases and livestock product supply and countermeasures. Korean J. Environ. Agric. 1, 33-75. https://doi.org/10.5338/KJEA.2010.29.1.033
  25. Mori, T., Miyazaki, Y., 2002. Effect of softwood thin veneers in tatami on the activity of the house dust mite Dermato- phagoides pteronyssinus. J. Wood Sci. 48, 163-164. https://doi.org/10.1007/BF00767295
  26. Park, J.M., Ryu, J.Y., Song, H.J., 2008. Efficient management of zoonoses. Korean J. Vet. Serv. 31, 425-431.
  27. Parry, N.M., 2020. COVID-19 and pets: when pandemic meets panic. Forensic. Sci. Int Rep. 2, 100090.
  28. Schofield, S., Tepper, M., Gadawski, R., 2007. Laboratory andfield evaluation of the impct of exercise on the performance ofregular and polymer-based deet repellents. J. Med. Entomol. 44, 1026-1031. https://doi.org/10.1093/jmedent/44.6.1026
  29. Whittaker, R.H., Feeny, P.P., 1971. Allelochemics: chemical Interactions between Species. AAAS 171, 757-770. https://doi.org/10.1126/science.171.3973.757