Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
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Mitochondrial oxidative damage by co-exposure to bisphenol A and acetaminophen in rat testes and its amelioration by melatonin

  • Hina Rashid (Department of Pharmacology and Toxicology, College of Pharmacy, Jazan University) ;
  • Mohammad Suhail Akhter (Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, Jazan University) ;
  • Saeed Alshahrani (Department of Pharmacology and Toxicology, College of Pharmacy, Jazan University) ;
  • Marwa Qadri (Department of Pharmacology and Toxicology, College of Pharmacy, Jazan University) ;
  • Yousra Nomier (Department of Pharmacology and Toxicology, College of Pharmacy, Jazan University) ;
  • Maryam Sageer (Department of Pharmacology and Toxicology, College of Pharmacy, Jazan University) ;
  • Andleeb Khan (Department of Pharmacology and Toxicology, College of Pharmacy, Jazan University) ;
  • Mohammad F. Alam (Department of Pharmacology and Toxicology, College of Pharmacy, Jazan University) ;
  • Tarique Anwer (Department of Pharmacology and Toxicology, College of Pharmacy, Jazan University) ;
  • Razan Ayoub (Department of Pharmacology and Toxicology, College of Pharmacy, Jazan University) ;
  • Rana J. H. Bahkali (Department of Pharmacology and Toxicology, College of Pharmacy, Jazan University)
  • Received : 2022.07.27
  • Accepted : 2023.01.05
  • Published : 2023.03.31
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Abstract

Objective: Human exposure to multiple xenobiotics, over various developmental windows, results in adverse health effects arising from these concomitant exposures. Humans are widely exposed to bisphenol A, and acetaminophen is the most commonly used over-the-counter drug worldwide. Bisphenol A is a well-recognized male reproductive toxicant, and increasing evidence suggests that acetaminophen is also detrimental to the male reproductive system. The recent recognition of male reproductive system dysfunction in conditions of suboptimal reproductive outcomes makes it crucial to investigate the contributions of toxicant exposures to infertility and sub-fertility. We aimed to identify toxicity in the male reproductive system at the mitochondrial level in response to co-exposure to bisphenol A and acetaminophen, and we investigated whether melatonin ameliorated this toxicity. Methods: Male Wistar rats were divided into six groups (n=10 each): a control group and groups that received melatonin, bisphenol A, acetaminophen, bisphenol A and acetaminophen, and bisphenol A and acetaminophen with melatonin treatment. Results: Significantly higher lipid peroxidation was observed in the testicular mitochondria and sperm in the treatment groups than in the control group. Levels of glutathione and the activities of catalase, glutathione peroxidase, glutathione reductase, and manganese superoxide dismutase decreased significantly in response to the toxicant treatments. Likewise, the toxicant treatments significantly decreased the sperm count and motility, while significantly increasing sperm mortality. Melatonin mitigated the adverse effects of bisphenol A and acetaminophen. Conclusion: Co-exposure to bisphenol A and acetaminophen elevated oxidative stress in the testicular mitochondria, and this effect was alleviated by melatonin.

Keywords

Acknowledgement

This work was supported by Jazan University through the Future Scientist program (grant no. FS10-088).

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