BMB Reports

생화학분자생물학회 (Korean Society for Biochemistry and Molecular Biology)
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Tat-Thioredoxin-like protein 1 attenuates ischemic brain injury by regulation of MAPKs and apoptosis signaling

  • Hyun Ju Cha (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Won Sik Eum (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Gi Soo Youn (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Jung Hwan Park (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Hyeon Ji Yeo (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Eun Ji Yeo (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Hyun Jung Kwon (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Lee Re Lee (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Na Yeon Kim (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Su Yeon Kwon (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Yong-Jun Cho (Department of Neurosurgery, Hallym University Medical Center) ;
  • Sung-Woo Cho (Department of Biochemistry and Molecular Biology, University of Ulsan College of Medicine) ;
  • Oh-Shin Kwon (School of Life Sciences, College of Natural Sciences Kyungpook National University) ;
  • Eun Jeong Sohn (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Dae Won Kim (Department of Biochemistry and Molecular Biology, Research Institute of Oral Sciences, College of Dentistry, Gangneung-Wonju National University) ;
  • Duk-Soo Kim (Department of Anatomy and BK21 FOUR Project, College of Medicine, Soonchunhyang University) ;
  • Yu Ran Lee (Department of Anatomy and BK21 FOUR Project, College of Medicine, Soonchunhyang University) ;
  • Min Jea Shin (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Soo Young Choi (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
  • 투고 : 2022.11.11
  • 심사 : 2022.12.22
  • 발행 : 2023.04.30
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초록

Thioredoxin-like protein 1 (TXNL1), one of the thioredoxin superfamily known as redox-regulator, plays an essential in maintaining cell survival via various antioxidant and anti-apoptotic mechanisms. It is well known that relationship between ischemia and oxidative stress, however, the role of TXNL1 protein in ischemic damage has not been fully investigated. In the present study, we aimed to determine the protective role of TXNL1 against on ischemic injury in vitro and in vivo using cell permeable Tat-TXNL1 fusion protein. Transduced Tat-TXNL1 inhibited ROS production and cell death in H2O2-exposed hippocampal neuronal (HT-22) cells and modulated MAPKs and Akt activation, and pro-apoptotic protein expression levels in the cells. In an ischemia animal model, Tat-TXNL1 markedly decreased hippocampal neuronal cell death and the activation of astrocytes and microglia. These findings indicate that cell permeable Tat-TXNL1 protects against oxidative stress in vitro and in vivo ischemic animal model. Therefore, we suggest Tat-TXNL1 can be a potential therapeutic protein for ischemic injury.

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과제정보

This research was supported by Basic Science Research Program (2018R1D1A3B07049265 & 2019R1A6A1A11036849) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education.

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