Journal of Korean Society on Water Environment (한국물환경학회지)

Korean Society on Water Environment (한국물환경학회)
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Electroencephalography (EEG) based Toxicity Test of Algae Organic Matter on Zebrafish

조류기인 유기물질의 제브라피쉬에 대한 뇌파측정기반 독성평가

  • Oh Sehyun (Department of Civil and Environmental Engineering, Daejeon University) ;
  • Jang hyeongjun (Department of Civil and Environmental Engineering, Daejeon University) ;
  • Cho Yunchul (Department of Civil and Environmental Engineering, Daejeon University)
  • 오세현 (대전대학교 토목환경공학과) ;
  • 장형준 (대전대학교 토목환경공학과) ;
  • 조윤철 (대전대학교 토목환경공학과)
  • Received : 2023.04.20
  • Accepted : 2023.05.23
  • Published : 2023.05.30
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Abstract

Harmful algae blooms have become a serious environmental problem in major river basins in Korea. They are known to produce various algal organic matters (AOMs) including intracellular organic matters (IOMs) and extracellular organic matters (EOMs). Generally AOMs cannot be easily removed by coagulation/flocculation process in conventional drinking water plants. AOMs produced by blue-green algae also include various toxins such as Microcystins, Anatoxin-a, and Saxitoxin known to have harmful effects on living organisms in aquatic environment. In this study, toxic effects of EOMs produced by three different algae species (Microcystis sp., Anabaena sp., and Oscillatoria sp.) on zebrafish were investigated using electroencephalography (EEG) recording method, a technology for recording brain activity. Electroencephalographic changes in zebrafish revealed that a low EOM had a negative effect on zebrafish compared to both Anabaena sp. and Oscillatoria sp. at 30 ppm EOM exposures. This result might be due to Microcystins present in EOMs produced by Microcystis sp. As a result of power spectrum density anallysis, exposure to EOMs produced by Microcystis sp. caused a state of vigilance in zebrafish. This EEG based toxicity test can be used to examine effects of harmful materials at low levels on living organisms in an aquatic system.

Keywords

Acknowledgement

본 연구는 2022년 정부(과학기술정보통신부)의 재원으로 한국연구재단의 지원을 받아 수행된 연구(NO. NRF2022R1F1A1074684)입니다, 그리고 EEG 분석에 도움을 주신 제핏(Daegu, Korea)에 감사드립니다.

References

  1. Afrikanova, T., Serruys, A. S. K., Buenafe, O. E. M., Clinckers, R., Smolders, I., de Witte, P. A. M., Crawford, A. D., and Esguerra, C. V. (2013). Validation of the zebrafish pentylenetetrazol seizure model: Locomotor versus electrographic responses to Antiepileptic Drugs, PLoS ONE, 8(1), 54166.
  2. Ali, S., Champagne, D. L., Spaink, H. P., and Richardson, M. K. (2011), Zebrafish embryos and larvae: A new generation of disease models and drug screens, Birth Defects Research Part C: Embryo Today: Reviews, 93(2), 115-133. https://doi.org/10.1002/bdrc.20206
  3. Cardozo, K. H. M., Guaratin, T., Barros, M. P., Falcao, V. R., Tonon, A. P., and Lopes, N. P. (2007). Metabolites from algae with economical impact, Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 146(1-2), 60-78. https://doi.org/10.1016/j.cbpc.2006.05.007
  4. Chen, J., Gao, N., Li, L., Zhu, M., Yang, J., and Lu, X. (2017). Disinfection by-product formation during chlor(am)ination of algal organic matters (AOM) extracted from microcystis aeruginosa: Effect of growth phases, aom and bromide concentration, Environmental Science and Pollution Research, 24(9), 1-10. https://doi.org/10.1007/s11356-017-8515-6
  5. Dai, R., Xiong, Y., Ma, Y., and Tang, T. (2020). Algae removal performance of UV-radiation-enhanced coagulation for two representative algal species, Science of The Total Environment, 745, 141013.
  6. Dai, Y. J., Jia, Y. F., Chen, N., Bian, W. P., Li, Q. K., Ma, Y. B., Chen, Y. L., and Pei, D. S. (2014). Zebrafish as a model system to study toxicology, Environment Toxicology and Chemistry, 33(1), 11-17. https://doi.org/10.1002/etc.2406
  7. Hamid, N. H. A., Sulaiman, N., Aris, S. A. M., Murat, Z. H., and Taib, M. N. (2010). Evaluation of human stress using EEG Power Spectrum, 2010 6th International Colloquium on Signal Processing & its Applications, Malacca, Malaysia, 1-4.
  8. Hong, M. H. (2018). Characteristics of chlorination byproducts formation by microcystis sp. and coelastrum sp. of Hoeya reservoir, Master's Thesis, Pusan National University, Busan, Korea [Korean Literature]
  9. Howe, K., Clark, M. D., Torroja, C. F., Torrance, J., Berthelot, C., Muffato, M., Collins, J. E., Humphray, S., McLaren, K., Matthews, L., McLaren, S., Sealy, I., Caccamo, M., Churcher, C., Scott, C., Barrett, J. C., Koch, R., Rauch, G-J., White, S., Chow, W., Kilian, B., Quintais, L, T., Guerra-Assunca-o, J, A., Zhou, Y., Gu, Y., Yen, J., Vogel, J-H., Eyre, T., Redmond, S., Banerjee, R., Chi, J., Fu, B., Langley, E., Maguire, S. F., Laird, G. K., Lloyd. D., Kenyon, E., Donaldson, S., Sehra, H., Almeida-King, J., Loveland, J., Trevanion, S., Jones, M., Quail, M., Dave Willey, D., Hunt, A., Burton, J., Sims, S., McLay, K., Plumb, B., Davis, J., Clee, C., Oliver, K., Clark, R., Riddle, C., Elliot, D., Threadgold, G., Harden, G., Ware, D., Begum, S., Mortimore, B., Kerry, G., Heath, P., Phillimore, B., Tracey, A., Corby, N., Dunn, M., Johnson, C., Wood, J., Clark, S., Pelan, S., Griffiths, G., Smith, M., Glithero, R., Howden, P., Barker, N., Lloyd, C., Stevens, C., Harley, J., Holt, K., Panagiotidis, G., Lovell, J., Beasley, H., Henderson, C., Gordon, D., Auger, K., Wright, D., Collins, J., Raisen, C., Dyer, L., Leung, K., Robertson, L., Ambridge, K., Leongamornlert, D., McGuire, S., Gilderthorp, R., Griffiths, C., Manthravadi, D., Nichol, S., Barker, G., Whitehead, S., Kay, M., Brown, J., Murnane, C., Gray, E., Humphries, M., Sycamore, N., Barker, D., Saunders, D., Wallis, J., Babbage, A., Hammond, S., Mashreghi-Mohammadi, M., Barr, L., Martin, S., Wray, P., Ellington, A., Matthews, N., Ellwood, M., Woodmansey, R., Clark, G., Cooper, J. D., Tromans, A., Grafham, D., Skuce, C., Pandian, R., Andrews, R., Harrison, E., Kimberley, A., Garnett, J., Fosker, N., Hall, R., Garner, P., Kelly, D., Bird, C., Palmer, S., Gehring, I., Berger, A., Dooley, C. M., Ersan-Urun, Z., Eser, C., Geiger, H., Geisler, M., Karotki, L., Kirn, A., Konantz, J., Konantz, M., Oberlander, M., Rudolph-Geiger, S., Teucke, M., Lanz, C., Raddatz, G., Osoegawa, K., Zhu, B., Rapp, A., Widaa, S., Langford, C., Yang, F., Schuster, S. C., Carter, N. P., Harrow, J., Ning, Z., Herrero, J., Searle, S. M. J., Enright, A., Geisler, R., Plasterk, R. H. A., Lee, C., Westerfield, M., de Jong, P. J., Zon, L. I., Postlethwait, J. H., Nusslein-Volhard, C., Hubbard, T. J. P., Crollius, H. R., Rogers, J., and Stemple, D. L. (2013). The zebrafish reference genome sequence and its relationship to the human genome, Nature, 496, 498-503. https://doi.org/10.1038/nature12111
  10. Hwang, K. S., Kan, H. M., Kim, S. S., Chae, J. S., Yang, J. Y., Shin, D. S., Ahn, S. H., Ahn, J. H., Cho, J. H., Jang, I. S., Shin, J. Y., Joo, J. Y., Kim, C. H., and Bae, M. A. (2020). Efficacy and pharmacokinetics evaluation of 4-(2-chloro-4-fluorobenzyl)-3-(2-thienyl)-1,2,4-oxadiazol-5 (4H)-one (GM-90432) as an anti-seizure agent, Neurochemistry International, 141, 104870.
  11. Kim, H. and Park, C. (2019). A Study on the removal of harmful compounds as algae by advanced oxidation processes, Proceedings of the 2019 Spring Co-Conference of the Korean Society on Water Environment and Korean Society of Water and Wastewater, Korean Society on Water Environment and Korean Society of Water and Wastewater, 216-217. [Korean Literature]
  12. Knyazev, G. G. (2012). EEG delta oscillations as a correlate of basic homeostatic and motivational processes, Neuroscience & Biobehavioral Reviews, 36(1), 677-695. https://doi.org/10.1016/j.neubiorev.2011.10.002
  13. Lee, I. J., Lee, C. G., Heo, S. N., and Lee, J. W. (2011). Analysis of anatoxin-a in aqueous and cyanobacterial samples from korean lakes by liquid chromatography with fluorescence detection, Analytical Science and Technology, 24(3), 225-230. [Korean Literature] https://doi.org/10.5806/AST.2011.24.3.225
  14. Lee, J. H. and Shin, G. A. (2017). Quantitative risk assessment of the adverse effects due to exposure to Cyanobacteria toxin (Microcystin-LR) through drinking water in the Nakdong river watershed, Journal of Environmental Science International, The Korean Environmental Sciences Society, 26(3), 345-362. [Korean Literature] https://doi.org/10.5322/JESI.2017.26.3.345
  15. Lee, Y. H., Lee, K. J., Jang, J .W., Lee, S. I., and Kim, S. H. (2020). An EEG system to detect brain signals from multiple adult zebrafish, Biosensors and Bioelectronics 164, 112315.
  16. Lee, Y. H., Seo, H. W., Lee, K. J., Jang, J. W., and Kim, S. H. (2020). A Microfluidic system for stable and continuous EEG monitoring from multiple larval zebrafish, Sensors, 20(20), 5903.
  17. Levitas-Djerbi, T. and Appelbaum, L. (2017). Modeling sleep and neuropsychiatric disorders in zebrafish, Current Opinion in Neurobiology, 44, 89-93. https://doi.org/10.1016/j.conb.2017.02.017
  18. Nguyen, M. L., Westerhoff, P., Baker, L., Hu, Q., Esparza-Soto, M., and Sommerfeld, M. (2005). Characteristics and reactivity of algaeproduced dissolved organic carbon, Journal of Environmental Engineering, 131(11), 1574-1582. https://doi.org/10.1061/(ASCE)0733-9372(2005)131:11(1574)
  19. Oathes, D. J., Ray, W. J., Yamasaki, A. S., Borkovec, T. D., Castonguay, L. G., Newman, M. G., and Nitschke, J. (2008). Worry, generalized anxiety disorder, and emotion: Evidence from the EEG gamma band, Biological Psychology, 79(2), 165-170. https://doi.org/10.1016/j.biopsycho.2008.04.005
  20. Oberemm, A., Fastner, J., and Steinberg, C. E. W. (1997). Effects of microcystin-LR and cyanobacterial crude extracts on embryo-larval development of zebrafish (Danio rerio), Water Research, 31(11), 2918-2921. https://doi.org/10.1016/S0043-1354(97)00120-6
  21. Paralkar, A. and Edzwald, J. K. (1996). Effect of ozone on EOM and coagulation, Journal American Water Works Association, 88, 143-154. https://doi.org/10.1002/j.1551-8833.1996.tb06540.x
  22. Park, S. J., Cha I. K., and Yoon, T. I. (2005). Removal of Algogenic organic matter in drinking water treatment process, Journal of Korean Society of Environmental Engineers, 27(4), 377-384. [Korean Literature]
  23. Petroff, R., Murias, M., Grant, K.S., Crouthamel, B., McKain, N Shum, S., Jing, J., Isoherranen, N., and Burbacher, T. M. (2020). Power spectrum analysis of EEG in a translational nonhuman primate model after chronic exposure to low levels of the common marine neurotoxin, domoic acid, NeuroToxicology, 80, 124-129. https://doi.org/10.1016/j.neuro.2020.07.006
  24. Wang, J. (2022). Characteristics of algae-derived organic matter and its role in formation of disinfection by-products, Master's Thesis, Daejeon University, Daejeon, Korea. [Korean Literature]
  25. Yoon, H. J., Lim, Y. S., Maeng, S. K., Hong, Y. S., Byun, S. K., Kim, H. C., Kim, B. S., and Kim, S. P. (2020). Impact of DBPs on the fate of zebrafish: Behavioral and lipid profile changes, Membrane and Water Treatment, 11(6), 391-398. https://doi.org/10.12989/MWT.2020.11.6.391
  26. Zhang, Y., Tian, J. Y., Nan, J., Gao, S. S., Liang, H., Wang, M. L., and Li, G. B. (2011). Effect of PAC addition on immersed ultrafiltration for the treatment of algal-rich water, Journal of Hazardous Materials, 186(2-3), 1415-1424. https://doi.org/10.1016/j.jhazmat.2010.12.015