Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Expression of heat shock protein genes in Simmental cattle exposed to heat stress

  • Luis Felipe Guzman (National Genetic Resources Center, National Institute of Forestry, Agricultural and Livestock Researches) ;
  • Guillermo Martinez-Velazquez (C.E. Santiago Ixcuintla, National Institute of Forestry, Agricultural and Livestock Researches) ;
  • Fernando Villasenor-Gonzalez (C.E. Centro Altos de Jalisco, National Institute of Forestry, Agricultural and Livestock Researches) ;
  • Vicente Eliezer Vega-Murillo (Faculty of Veterinary Medicine and Zootechnics, Universidad Veracruzana) ;
  • Jose Antonio Palacios-Franquez (C.E. Santiago Ixcuintla, National Institute of Forestry, Agricultural and Livestock Researches) ;
  • Angel Rios-Utrera (C.E. La Posta, National Institute of Forestry, Agricultural and Livestock Researches) ;
  • Moises Montano-Bermudez (National Disciplinary Research Center for Physiology and Animal Improvement, National Institute of Forestry, Agricultural and Livestock Researches)
  • Received : 2022.07.08
  • Accepted : 2022.12.14
  • Published : 2023.05.01
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Abstract

Objective: In tropical, subtropical and arid zones, heat stress is the main cause of productivity reduction in cattle. When climate stressors occur, animals become thermal adapted through differential expression of some genes, including heat shock proteins (HSP) family. The aim of this study was to determine levels of expression of HSP60, HSP70, and HSP90 genes in Simmental cattle raised in tropical environments of Mexico. Methods: In this study, expression of HSP60, HSP70, and HSP90 genes was analyzed in 116 Simmental cattle from three farms with tropical climate located in western Mexico. Animals were sampled twice a day, in the morning and noon. Gene expression was evaluated by quantitative polymerase chain reaction using probes marked with fluorescence. The MIXED procedure of SAS with repeated measures was used for all statistical analysis. Results: HSP60 gene expression differences were found for sex (p = 0.0349). HSP70 gene differences were detected for sampling hour (p = 0.0042), farm (p<0.0001), sex (p = 0.0476), and the interaction sampling hour×farm (p = 0.0002). Gene expression differences for HSP90 were observed for farm (p<0.0001) and year (p = 0.0521). HSP70 gene showed to be a better marker of heat stress than HSP60 and HSP90 genes. Conclusion: Expression of HSP70 gene in Simmental herds of the tropical region of western México was different during early morning and noon, but the expression of the HSP60 and HSP90 genes was similar. Identification of resilient animals to heat stress will be useful in the genetic improvement of the Simmental breed.

Keywords

Acknowledgement

Our thanks to the producers for providing the animals for this study and the Mexican Livestock Association of Simmental and Simbrah Cattles.

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