Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Protodioscin protects porcine oocytes against H2O2-induced oxidative stress during in vitro maturation

  • So-Hee Kim (Stem Cell Research Center, Jeju National University) ;
  • Seung-Eun Lee (Stem Cell Research Center, Jeju National University) ;
  • Jae-Wook Yoon (Stem Cell Research Center, Jeju National University) ;
  • Hyo-Jin Park (Stem Cell Research Center, Jeju National University) ;
  • Seung-Hwan Oh (Stem Cell Research Center, Jeju National University) ;
  • Do-Geon Lee (Stem Cell Research Center, Jeju National University) ;
  • Da-Bin Pyeon (Stem Cell Research Center, Jeju National University) ;
  • Eun-Young Kim (Stem Cell Research Center, Jeju National University) ;
  • Se-Pill Park (Stem Cell Research Center, Jeju National University)
  • Received : 2022.07.20
  • Accepted : 2022.11.07
  • Published : 2023.05.01
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Abstract

Objective: The present study investigated whether protodioscin (PD), a steroidal saponin mainly found in rhizome of Dioscorea species, alleviates oxidative stress-induced damage of porcine oocytes during in vitro maturation. Methods: Oocytes were treated with different concentrations of PD (0, 1, 10, 100, and 200 µM) in the presence of 200 µM H2O2 during in vitro maturation. Following maturation, spindle morphology and mitogen-activated protein kinase activity was assessed along with reactive oxygen species level, GSH activity, and mRNA expression of endogenous antioxidant genes at the MII stage. On the day 7 after parthenogenetic activation, blastocyst formation rate was calculated and the quality of embryo and mRNA expression of development-related genes was evaluated. Results: Developmental competence was significantly poorer in the 0 µM PD-treated (control) group than in the non-treated (normal) and 10 µM PD-treated (10PD) groups. Although the reactive oxygen species level did not significantly differ between these three groups, the glutathione level and mRNA expression of antioxidant genes (superoxide dismutase 1 [SOD1], SOD2, nuclear factor erythroid 2-related factor 2 [Nrf2], and hemo oxygenase-1 [HO-1]) were significantly higher in the normal and 10PD groups than in the control group. In addition, the percentage of oocytes with defective spindle and abnormal chromosomal alignment was significantly lower and the ratio of phosphorylated p44/42 to total p44/42 was significantly higher in the normal and 10PD groups than in the control group. The total cell number per blastocyst was significantly higher in the 10PD group than in the control group. The percentage of apoptotic cells in blastocysts was highest in the control group; however, the difference was not significant. mRNA expression of development-related genes (POU domain, class 5, transcription factor 1 [POU5F1], caudal type homeobox 2 [CDX2], Nanog homeobox [NANOG]) was consistently increased by addition of PD. Conclusion: The PD effectively improves the developmental competence and quality of blastocysts by protecting porcine oocytes against oxidative stress.

Keywords

Acknowledgement

This study was supported by the Research Center for Production Management and Technical Development for High Quality Livestock Products through the Agriculture, Food and Rural Affairs Research Center Support Program for Educating Creative Global Leader, Ministry of Agriculture, Food and Rural Affairs, Korea (Grant No. 715003-07).

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