Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Methanol extract of Myelophycus caespitosus ameliorates oxidative stress-induced cytotoxicity in C2C12 murine myoblasts via activation of heme oxygenase-1

  • Cheol Park (Division of Basic Sciences, College of Liberal Studies, Dong-Eui University) ;
  • Hyun Hwangbo (Department of Biochemistry, Dong-Eui University College of Korean Medicine) ;
  • Min Ho Han (National Marine Biodiversity Institute of Korea) ;
  • Jin-Woo Jeong (Honam National Institute of Biological Resources) ;
  • Suengmok Cho (Department of Food Science and Technology, Institute of Food Science, Pukyong National University) ;
  • Gi-Young Kim (Department of Marine Life Science, Jeju National University) ;
  • Hye-Jin Hwang (Department of Food and Nutrition, College of Nursing, Healthcare Sciences & Human Ecology, Dong-Eui University) ;
  • Yung Hyun Choi (Department of Biochemistry, Dong-Eui University College of Korean Medicine)
  • Received : 2022.11.02
  • Accepted : 2022.11.15
  • Published : 2023.01.30
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Abstract

Myelophycus caespitosus, a brown alga belonging to genus Myelophycus, has been traditionally used as a food and medicinal resource in Northeastern Asia. However, few studies have been conducted on its pharmacological activity. In this study, we evaluated whether methanol extract of M. caespitosus (MEMC) could protect against oxidative damage caused by hydrogen peroxide (H2O2) in C2C12 murine myoblasts. Our results revealed that MEMC could suppress H2O2-induced growth inhibition and DNA damage while blocking the production of reactive oxygen species. In H2O2-treated cells, cell cycle progression was halted at the G2/M phase, accompanied by changes in expression of key cell cycle regulators. However, these effects were attenuated by MEMC. In addition, we found that MEMC protected cells from induction of apoptosis associated with mitochondrial impairment caused by H2O2 treatment. Furthermore, MEMC enhanced the phosphorylation of nuclear factor-erythroid-2 related factor 2 (Nrf2) and expression and activity of heme oxygenase-1 (HO-1) in H2O2-treaetd C2C12 myoblasts. However, such anti-apoptotic and cytoprotective effects of MEMC were greatly abolished by HO-1 inhibitor, suggesting that MEMC could increase Nrf2-mediated activity of HO-1 to protect C2C12 myoblasts from oxidative stress.

Keywords

Acknowledgement

This research was supported by Korea Institute of Marine Science & Technology Promotion (KIMST) funded by the Ministry of Oceans and Fisheries, Korea (20220488).

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