생태와환경 (Korean Journal of Ecology and Environment)

  • 제56권1호
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  • Pages.1-13
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  • 2023
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  • 2288-1115(pISSN)
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  • 2288-1123(eISSN)
한국수생태학회 (The Korean Society of Limnology)
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담수환경에서 eDNA와 eRNA를 이용한 Microcystin 합성 남조류 탐색 및 세포 내 Microcystin 생합성 활성 변화

Detection of Microcystin Synthetic Cyanobacteria and Variation of Intracellular Microcystin Synthesis Using by eDNA and eRNA in Freshwater Ecocystem

  • 김건희 (건국대학교 상허생명과학대학 휴먼앤에코케어센터) ;
  • 박채홍 (건국대학교 상허생명과학대학 휴먼앤에코케어센터) ;
  • 조현진 (전남대학교 생물학과) ;
  • 권대률 (국립낙동강생물자원관 미생물연구실 원생생물연구팀) ;
  • 황순진 (건국대학교 환경보건과학과)
  • Keonhee Kim (Human and Eco Care Center, College of Sang-huh Life Science, Konkuk University) ;
  • Chaehong Park (Human and Eco Care Center, College of Sang-huh Life Science, Konkuk University) ;
  • Hyeonjin Cho (Department of Biology, Chonnam National University) ;
  • Daeryul Kwon (Protist Research Team, Microbial Department, Nakdonggang National Institute of Biological Resources) ;
  • Soon-Jin Hwang (Department of Environmental Health Science, Konkuk University)
  • 투고 : 2023.02.24
  • 심사 : 2023.03.16
  • 발행 : 2023.03.31
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초록

북한강 수역에서 가장 많이 검출되는 Microcystin (MC)을 대상으로 하여 MC 생합성 유전자(mcyA gene), 남조류 세포밀도, MC 농도 사이의 관계를 분석하여 RNA-MC 환산식을 도출하고 남조류 세포 내 존재하는 MC 농도를 예측하였다. 북한강 수역에서 mcyA 유전자는 묵현천 합류 이후 북한강 하류 지점에서 주로 발견되었으며 평균적으로 다른 지점보다 높은 copy number가 발견되었다. 북한강 상류 의암호 수역의 경우, 공지천 지점에서 mcyA 유전자 copy number가 증가하였으며 9월 이후 북한강 수역 전체에서 mcyA 유전자 copy number는 감소하였다. mcyA gene expression은 상류와 하류 수역의 시·공간적 차이가 존재하였으며 여름철 짧은 시기에 집중적으로 발현하였다. mcyA gene expression 양은 MC 농도와 상관성이 매우 높을 뿐만 아니라 MC을 생합성하는 것으로 알려진 Microcystis aeruginosa와 Dolichospermum circinale의 세포밀도와도 통계적으로 유의한 상관성이 존재하였다. RNA-MC 관계를 기반으로 도출된 6개의 환산식은 통계적 유의성을 보이며(p<0.05) 0.9 이상의 높은 상관계수(r)를 나타냈다. eRNA에 존재하는 MC 생합성 유전자 발현량은 수중의 남조독소 물질 합성을 판단하고 유전자의 활성 정도를 빠르게 정량하여 MC 발생 조기경보에 충분히 활용할 수 있을 것으로 판단된다.

Targeting Microcystin (MC), which is most abundantly detected in the North-Han River water area, we analyzed the relationship between the MC biosynthesis gene (mcyA gene), cyanobacteria cell density, and MC concentration, derived an RNA-MC conversion formula, and derived the cyanobacteria. The concentration of MC present in cells was predicted. In the North-Han River waters, the mcyA gene was found mainly at downstream sites of the North-Han River after Muk-Hyeon Stream junction, and higher copy numbers were found on average than other sites. In the Uiam Lake waters upstream of the North-Han River, the mcyA gene copy number increased at the Kong-Ji Stream point, and after September, the mcyA gene copy number decreased throughout the North-Han River waters. The expression of the mcyA gene was concentrated in the short period of summer due to the spatio-temporal difference between upstream and downstream water bodies. The mcyA gene expression level was not only highly correlated with MC concentration, but also correlated with the cell density of Microcystis aeruginosa and Dolichospermum circinale, which are known to biosynthesize MC. Six conversion formulas derived based on the RNA-MC relationship showed statistical significance (p<0.05) and exhibited high correlation coefficients (r) of 0.9 or higher. The expression level of MC biosynthesis gene present in eRNA determines the synthesis of cyanotoxin substances in water, quickly quantifies gene activity, and can be fully utilized for early warning of MC development.

키워드

과제정보

이 논문은 2021학년도 건국대학교의 연구년교원 지원에 의하여 연구되었음.

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