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T Cell Microvilli: Finger-Shaped External Structures Linked to the Fate of T Cells

  • Hye-Ran Kim (School of Life Sciences, Gwangju Institute of Science and Technology (GIST)) ;
  • Jeong-Su Park (School of Life Sciences, Gwangju Institute of Science and Technology (GIST)) ;
  • Won-Chang Soh (School of Life Sciences, Gwangju Institute of Science and Technology (GIST)) ;
  • Na-Young Kim (School of Life Sciences, Gwangju Institute of Science and Technology (GIST)) ;
  • Hyun-Yoong Moon (School of Life Sciences, Gwangju Institute of Science and Technology (GIST)) ;
  • Ji-Su Lee (School of Life Sciences, Gwangju Institute of Science and Technology (GIST)) ;
  • Chang-Duk Jun (School of Life Sciences, Gwangju Institute of Science and Technology (GIST))
  • Received : 2022.12.25
  • Accepted : 2023.02.11
  • Published : 2023.02.28
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Abstract

Microvilli are outer membrane organelles that contain cross-linked filamentous actin. Unlike well-characterized epithelial microvilli, T-cell microvilli are dynamic similar to those of filopodia, which grow and shrink intermittently via the alternate actin-assembly and -disassembly. T-cell microvilli are specialized for sensing Ags on the surface of Ag-presenting cells (APCs). Thus, these finger-shaped microprotrusions contain many signaling-related proteins and can serve as a signaling platforms that induce intracellular signals. However, they are not limited to sensing external information but can provide sites for parts of the cell-body to tear away from the cell. Cells are known to produce many types of extracellular vesicles (EVs), such as exosomes, microvesicles, and membrane particles. T cells also produce EVs, but little is known about under what conditions T cells generate EVs and which types of EVs are released. We discovered that T cells produce few exosomes but release large amounsts of microvilli-derived particles during physical interaction with APCs. Although much is unanswered as to why T cells use the same organelles to sense Ags or to produce EVs, these events can significantly affect T cell fate, including clonal expansion and death. Since TCRs are localized at microvilli tips, this membrane event also raises a new question regarding long-standing paradigm in T cell biology; i.e., surface TCR downmodulation following T cell activation. Since T-cell microvilli particles carry T-cell message to their cognate partner, these particles are termed T-cell immunological synaptosomes (TISs). We discuss the potential physiological role of TISs and their application to immunotherapies.

Keywords

Acknowledgement

This work was supported by the Creative Research Initiative Program (2015R1A3A2066253) through National Research Foundation (NRF) grants funded by the Ministry of Science and ICT (MSIT), the Basic Science Program (2022R1A2C4002627) through National Research Foundation (NRF) grants funded by the Ministry of Education (MOE), and supported by Global University Project (GUP), GIST Research Institute (GRI) IBBR grant funded by the GIST (in 2021-2022), and the Joint Research Project of Institutes of Science and Technology (2021-2022), Korea.

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