식물병연구 (Research in Plant Disease)

한국식물병리학회 (The Korean Society of Plant Pathology)
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인공접종을 통한 국내 야생 장미과 식물의 화상병 감수성 검정

Determination of Fire Blight Susceptibility on Wild Rosaceae Plants in Korea by Artificial Inoculation

  • 박인웅 (서울대학교 식물면역연구센터) ;
  • 송유림 (경희대학교 원예생명공학과) ;
  • 오엄지 (경희대학교 그린바이오과학원) ;
  • 김요엘 (경희대학교 그린바이오과학원) ;
  • 황인선 (경희대학교 원예생명공학과) ;
  • 전미진 (국립생물자원관 미생물자원과) ;
  • 안초롱 (국립생물자원관 미생물자원과) ;
  • 김진숙 (한반도식물다양성연구소) ;
  • 김순옥 (국립생물자원관 미생물자원과) ;
  • 오창식 (서울대학교 식물면역연구센터)
  • In Woong Park (Plant Immunity Research Center, Seoul National University) ;
  • Yu-Rim Song (Department of Horticultural Biotechnology, Kyung Hee University) ;
  • Eom-Ji Oh (Graduate School of Green-Bio Science, Kyung Hee University) ;
  • Yoel Kim (Graduate School of Green-Bio Science, Kyung Hee University) ;
  • In Sun Hwang (Department of Horticultural Biotechnology, Kyung Hee University) ;
  • Mi-Jin Jeon (Microorganism Resources Division, National Institute of Biological Resources) ;
  • Chorong Ahn (Microorganism Resources Division, National Institute of Biological Resources) ;
  • Jin-Suk Kim (Korean Plant Diversity Institute) ;
  • Soonok Kim (Microorganism Resources Division, National Institute of Biological Resources) ;
  • Chang-Sik Oh (Plant Immunity Research Center, Seoul National University)
  • 투고 : 2022.12.23
  • 심사 : 2022.12.28
  • 발행 : 2023.03.31
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초록

Erwinia amylovora에 의해 발생하는 화상병은 2015년 국내에서 처음 보고된 이후 전국적으로 확산되고 있다. 화상병은 벌과 같은 화분매개충, 강한 바람을 동반한 강수와 이병주 전정 작업에 의한 전파 등 많은 경로를 통해 병원균 감염이 진행되어 발생한다. 이 외에도 자생하는 개야광나무속, 산사나무속, 피라칸다속, 벚나무속, 마가목속 등의 장미과 식물이 E. amylovora의 기주식물과 전파원이 될 수 있음이 보고되어 있다. 그러나, 국내에서는 아직까지 자생하고 있는 장미과 식물의 화상병 감수성에 관한 연구가 많이 다뤄지지 않은 상황이다. 본 연구에서는 2020년부터 2022년까지 3년간 국내에서 채집한 야생 장미과 식물 10속 25종을 대상으로 화상병 감수성 검정을 진행하였다. 108 cfu/ml 농도의 E. amylovora 균액을 이용하여 채집한 식물의 꽃, 잎과 가지, 과실에 접종하고, 화상병 유사 병징 발현을 관찰하고 PCR을 통해 접종 조직 내의 E. amylovora 검출 여부를 분석하였다. 그 결과, 병징 발현과 병원균 검출 결과가 동일한 14종의 야생 장미과 식물을 확인하였다. 이러한 결과는 향후 적절한 화상병 방제 정책 수립에 기여할 수 있을 것으로 생각된다.

The fire blight caused by Erwinia amylovora (Ea) is a devastating disease of Rosaceae plants, including commercially important apple and pear trees. Since the first report in Korea in May 2015, it has been spreading to neighboring regions gradually. Host plants can be infected by pollinators like bees, rainfall accompanied by wind, and cultural practices such as pruning. Many studies have revealed that wild Rosaceae plants such as Cotoneaster spp., Crataegus spp., Pyracantha spp., Prunus spp., and Sorbus spp. can be reservoirs of Ea in nature. However, wild Rosaceae plants in Korea have not been examined yet whether they are susceptible to fire blight. Therefore, the susceptibility to fire blight was examined with 25 species in 10 genera of wild Rosaceae plants, which were collected during 2020-2022, by artificial inoculation. Bacterial suspension (108 cfu/ml) of Ea type strain TS3128 was inoculated artificially in flowers, leaves, stems, and fruits of each plant species, and development of disease symptoms were monitored. Moreover, the presence of Ea bacteria from inoculated samples were checked by conventional polymerase chain reaction. Total 14 species of wild Rosaceae plants showed disease symptoms of fire blight, and Ea bacteria were detected inside of inoculated plant parts. These results suggest that wild Rosaceae plants growing nearby commercial apple and pear orchards in Korea can be Ea reservoirs, and thus they should be monitored regularly to minimize the damage by Ea infection and spreading.

키워드

과제정보

This research was supported by the National Institute of Biological Resources, Ministry of Environment of Republic of Korea (NIBR202112101, NIBR202213101) and Bio & Medical Technology Development Program of the National Research Foundation (NRF) (No. 2021M3A9I5021437).

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