Applied Chemistry for Engineering (공업화학)

The Korean Society of Industrial and Engineering Chemistry (한국공업화학회)
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Effect of Tryptone and Tungsten in Medium on Syngas Fermentation Using Clostridium ljundahlii

Clostridium ljundahlii를 이용한 합성가스 발효에서 배지 내 tryptone 및 tungsten의 영향

  • Soeun Park (Research Center of Chemical Technology, Hankyong National University) ;
  • Young-Kee Kim (Department of Chemical Engineering, Hankyong National University)
  • 박소은 (한경대학교 화학기술연구소) ;
  • 김영기 (한경대학교 화학공학전공)
  • Received : 2023.03.06
  • Accepted : 2023.03.23
  • Published : 2023.06.10
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Abstract

In this study, an experiment was conducted to investigate the effect of the concentrations of tryptone, an organic nitrogen supplement, and sodium tungstate on the growth of microbial and the production of acetic acid and ethanol in the culture of Clostridium ljungdahlii. Microbial growth increased by 144.6%, and ethanol and acetic acid production improved by 8.6% and 36.7%, respectively, when 2.5 g/L of tryptone was added to the medium of the control experiment (0 g/L tryptone). In the experiment with 1 µM Na2WO4·2H2O, which is 100 times higher than the condition of the medium used in the control experiment (0.01 µM Na2WO4·2H2O), there was no significant difference in microbial growth or total production of C2 metabolites, but ethanol production increased and acetic acid production decreased. As a result, the ethanol/acetic acid production ratio increased significantly from 0.24 in the control experiment to 0.56.

본 연구에서는 Clostridium ljungdahlii 배양에서 유기질소공급물인 tryptone과 sodium tungstate의 배양액 내 농도가 균주 성장과 아세트산, 에탄올 생산에 대한 영향을 확인하기 위한 실험을 수행하였다. 대조군 실험의 배지 조건(0 g/L tryptone)보다 tryptone 2.5 g/L를 투입한 경우 균주 성장이 144.6% 증가하였고, 에탄올 및 아세트산 생산은 각각 8.6%와 36.7% 향상되었다. 대조군 실험에 사용된 배지의 조건(0.01 µM Na2WO4·2H2O)보다 100배인 1 µM Na2WO4·2H2O를 사용한 실험군에서, 균주 성장과 C2 대사산물의 총생산량에는 큰 차이가 없지만, 에탄올 생산 증가하고 아세트산 생산은 감소하여 에탄올/아세트산 생산비가 0.56로 대조군의 0.24에서 크게 증가하였다.

Keywords

Acknowledgement

이 논문은 교육부의 재원으로 한국연구재단-이공학개인기초연구지원사업(NRF-2018R1D1A1B07043323)의 지원을 받아 수행한 연구입니다.

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