Applied Chemistry for Engineering (공업화학)

The Korean Society of Industrial and Engineering Chemistry (한국공업화학회)
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Influence of pH on Chelation of BaCl2 and EDTA Using Isothermal Titration Calorimetry

등온적정열량계를 이용한 BaCl2와 EDTA 킬레이션 결합 반응의 pH 영향

  • Ga Eun Yuk (Department of Chemical Engineering, Kumoh National Institute of Technology) ;
  • Ji Woong Chang (Department of Chemical Engineering, Kumoh National Institute of Technology)
  • 육가은 (금오공과대학교 화학공학과) ;
  • 장지웅 (금오공과대학교 화학공학과)
  • Received : 2023.04.16
  • Accepted : 2023.04.26
  • Published : 2023.06.10
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Abstract

Isothermal titration calorimetry (ITC) is a useful technique to obtain thermodynamic binding properties such as enthalpy, Gibbs free energy, entropy, and stoichiometry of the chelation reaction. A single independent binding site model was used to evaluate the thermodynamic binding properties in BaCl2 and ethylenediaminetetraacetic acid (EDTA) in Trince and HEPES buffers. ITC enables us to elucidate the binding mechanism and find an optimal chelation condition for BaCl2 and EDTA in the pH range of 7~11. Chelation of BaCl2 and EDTA is a spontaneous endothermic reaction. As pH increased, entropic contributions dominated. The optimal pH range is narrow around pH 9.0, where 1:1 binding between BaCl2 and EDTA occurs.

등온 적정 열량계는 리간드-수용체 사이의 킬레이션 결합 반응의 엔탈피, 깁스에너지, 엔트로피, 화학양론 등 포함한 모든 열역학적 정보를 측정하는데 유용한 기술이다. 단일독립결합모델을 이용하여 Tricine과 HEPES 완충용엑에서의 BaCl2 와 ethylenediaminetetraacetic acid (EDTA)의 킬레이션 결합에서의 열역학적 정보를 획득하였다. 등온 적정 열량계를 이용하여 pH 7~11 영역에서의 킬레이션 결합의 메커니즘과 최적의 결합 조건을 확인하였다. BaCl2와 EDTA의 결합은 자발적인 발열반응이며 pH가 증가할수록 엔트로피적 영향이 높아진다. 1:1로 결합하는 pH 영역은 pH 9.0 근처에서 매우 좁은 영역에서 나타난다.

Keywords

Acknowledgement

이 연구는 금오공과대학교 학술연구비로 지원되었음(202002340001)

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