Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Effect of culture-promoting ingredients (CPI-107) on the culture of Mycobacterium tuberculosis

결핵균 배양에 대한 배양촉진물질(CPI-107)의 효과

  • Seung Cheol Kim (Institute for Immunology & Immunological Disease, Yonsei University College of Medicine) ;
  • Sezim Monoldorova (Department of Biomedical Laboratory Science, College of Software and Digital Healthcare Convergence, Yonsei University) ;
  • Bo-Young Jeon (Department of Biomedical Laboratory Science, College of Software and Digital Healthcare Convergence, Yonsei University)
  • 김승철 (연세대학교 의과대학 면역질환연구소) ;
  • 모놀도로바 세짐 (연세대학교 소프트웨어 디지털헬스케어융합대학 임상병리학과) ;
  • 전보영 ( 연세대학교 소프트웨어 디지털헬스케어융합대학 임상병리학과)
  • Received : 2022.12.06
  • Accepted : 2023.01.03
  • Published : 2023.03.30
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Abstract

Mycobacterium tuberculosis complex (M. tuberculosis complex) is a causative agent of contagious chronic disease in a wide range of mammalian hosts, mainly cattle, goat, pigs, wildlife, and humans. The definite diagnosis of tuberculosis is made based on culture of M. tuberculosis, but it takes a long time. In the present study, we analyzed whether the detection time of M. tuberculosis could be reduced when cultured in the medium containing the culture-promoting ingredients-107 (CPI-107) using the BacT/Alert 3D system, an automatic culture system. The time to detection (TTD) tended to decrease as the added concentration of CPI-107 increase. In the case of low numbers of M. tuberculosis, it decreased by 21.0% at 1.2 mg/mL of CPI-107 and by 15.9% in the case of high numbers of M. tuberculosis. In the culture using clinically isolated M. tuberculosis strains, the shortening of the culture time by CPI was more evident. In conclusion, the detection time of M. tuberculosis was shortened in the medium added with CPI-107, and this could be used for isolation, culture and drug susceptibility test of M. tuberculosis.

Keywords

Acknowledgement

이 논문은 한구연구재단 기초연구사업(NRF- 2013R1A1A2059687, NRF-2016R1A6A3A11933077)과 농림축산식품부의 재원으로 농림식품기술기획평가원의 가축질병대응기술고도화지원사업의 지원을 받아 연구되었음(과제번호: 122011-2).

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