Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effects of β-glucan with vitamin E supplementation on the growth performance, blood profiles, immune response, fecal microbiota, fecal score, and nutrient digestibility in weaning pigs

  • Tae Wook Goh (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University) ;
  • Hong Jun Kim (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University) ;
  • Kunyong Moon (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University) ;
  • Cheon Soo Kim (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University) ;
  • Yoo Yong Kim (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University)
  • Received : 2022.08.12
  • Accepted : 2022.10.25
  • Published : 2023.04.01
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Abstract

Objective: This study was conducted to evaluate effects of β-glucan with vitamin E supplementation on the growth performance, blood profiles, immune response, fecal microbiota, fecal score, and nutrient digestibility in weaning pigs. Methods: A total of 200 weaning pigs with an average body weight (BW) of 7.64±0.741 kg were allotted to five treatment groups and were divided based on sex and initial BW in four replicates with ten pigs per pen in a randomized complete block design. The experimental diets included a corn-soybean meal-based basal diet with or without 0.1% or 0.2% β-glucan and 0.02% vitamin E. The pigs were fed the diets for 6 weeks. A total of 15 barrows were used to evaluate the nutrient digestibility by the total collection method. The BW and feed intake were measured at the end of each phase. Blood samples were collected at the end of each phase, and fecal samples were collected at the end of the experiment. Results: The addition of β-glucan with vitamin E to weaning pig feed increased BW, average daily gain, and average daily feed intake. A significant decrease in yeast and mold and Proteobacteria and a tendency for Lactobacillus to increase compared to the control was shown when 0.1% β-glucan and 0.02% vitamin E were added. The fecal score in weaning pigs was lower in the treatments supplemented with 0.1% or 0.2% β-glucan and 0.02% vitamin E compared to the control. In addition, vitamin E was better supplied to weaning pigs by increasing the concentration of α-tocopherol in the blood of weaning pigs when 0.02% vitamin E was supplemented. However, there was no significant difference in either the immune response or nutrient digestibility. Conclusion: Inclusion of 0.1% β-Glucan with 0.02% vitamin E in a weaning pig's diet were beneficial to the growth performance of weaning pigs by improving intestinal microbiota and reducing the incidence of diarrhea.

Keywords

Acknowledgement

This research was supported by E&T Co., Ltd. (Daejeon, Korea)

References

  1. Unno T, Kim J, Guevarra RB, Nguyen SG. Effects of antibiotic growth promoter and characterization of ecological succession in swine gut microbiota. J Microbiol Biotechnol 2015;25:431-8. https://doi.org/10.4014/jmb.1408.08063 
  2. Eicher SD, McKee CA, Carroll JA, Pajor EA. Supplemental vitamin C and yeast cell wall beta-glucan as growth enhancers in newborn pigs and as immunomodulators after an endotoxin challenge after weaning. J Anim Sci 2006;84:2352-60. https://doi.org/10.2527/jas.2005-770 
  3. Vannucci L, Krizan J, Sima P, et al. Immunostimulatory properties and antitumor activities of glucans (Review). Int J Oncol 2013;43:357-64. https://doi.org/10.3892/ijo.2013.1974 
  4. Hahn TW, Lohakare JD, Lee SL, Moon WK, Chae BJ. Effects of supplementation of β-glucans on growth performance, nutrient digestibility, and immunity in weanling pigs. J Anim Sci 2006;84:1422-8. https://doi.org/10.2527/2006.8461422x 
  5. Stokes CR, Miller BG, Bailey M, Wilson AD, Bourne FJ. The immune response to dietary antigens and its influence on disease susceptibility in farm animals. Vet Immunol Immunopathol 1987;17:413-23. https://doi.org/10.1016/0165-2427(87)90158-9 
  6. Mahan DC. Assessment of the influence of dietary vitamin E on sows and offspring in three parities: reproductive performance, tissue tocopherol, and effects on progeny. J Anim Sci 1991;69:2904-17. https://doi.org/10.2527/1991.6972904x 
  7. Tengerdy RP, Mathias MM, Nockels CF. Effect of vitamin E on immunity and disease resistance. In: Prasad KN, editor. Vitamins, nutrition, and cancer. Basel, Switzerland: Karger; 1984. pp. 123-33. https://doi.org/10.1159/000409541 
  8. Bjorneboe A, Bjorneboe GEA, Drevon CA. Absorption, transport and distribution of vitamin E. J Nutr 1990;120:233-42. https://doi.org/10.1093/jn/120.3.233 
  9. Kim BG, Lindemann MD. A spreadsheet method for the experimental animal allotment. J Anim Sci 2007;85(Suppl 2):112. 
  10. NRC. Nutrient requirements of swine. 11th Ed. Washington, DC, USA: National Academy Press; 2012. 
  11. Yen HC, Lai WK, Lin CS, Chiang SH. Medium-chain triglyceride as an alternative of in-feed colistin sulfate to improve growth performance and intestinal microbial environment in newly weaned pigs. Anim Sci J 2015;86:99-104. https://doi.org/10.1111/asj.12248 
  12. McCarthy JF, Aherne FX, Okai DB. Use of HCL insoluble ash as an index material for determining apparent digestibility with pigs. Can J Anim Sci 1974;54:107-9. https://doi.org/10.4141/cjas74-016 
  13. Kim BG, Lee JW, Stein HH. Energy concentration and phosphorus digestibility in whey powder, whey permeate, and low-ash whey permeate fed to weanling pigs. J Anim Sci 2012;90:289-95. https://doi.org/10.2527/jas.2011-4145 
  14. AOAC. Official methods of analysis. 16th Edition. Washington, DC, USA: Association of Official Analytical Chemists; 1995. 
  15. Park JH, Lee SI, Kim IH. Effect of dietary β-glucan supplementation on growth performance, nutrient digestibility, and characteristics of feces in weaned pigs. J Appl Anim Res 2018;46:1193-7. https://doi.org/10.1080/09712119.2018.1481855 
  16. Luo J, Liu S, Yu B, et al. Beta-glucan from Agrobacterium sp. ZX09 improves growth performance and intestinal function in weaned piglets. J Anim Physiol Anim Nutr 2019;103:1818-27. https://doi.org/10.1111/jpn.13163 
  17. Li J, Li DF, Xing JJ, Cheng ZB, Lai CH. Effects of β-glucan extracted from Saccharomyces cerevisiae on growth performance, and immunological and somatotropic responses of pigs challenged with Escherichia coli lipopolysaccharide. J Anim Sci 2006;84;2374-81. https://doi.org/10.2527/jas.2004-541 
  18. Lee SI, Kim JK, Hancock JD, Kim IH. β-Glucan from mulberry leaves and curcuma can improve growth performance and nutrient digestibility in early weaned pigs. J Appl Anim Res 2017;45:209-14. https://doi.org/10.1080/09712119.2016.1141775 
  19. Zhou TX, Jung JH, Zhang ZF, Kim IH. Effect of dietary β-glucan on growth performance, fecal microbial shedding and immunological responses after lipopolysaccharide challenge in weaned pigs. Anim Feed Sci Technol 2013;179:85-92. https://doi.org/10.1016/j.anifeedsci.2012.10.008 
  20. Vetvicka V, Vannucci L, Sima P. The Effects of β-glucan on pig growth and immunity. Open Biochem J 2014;8:89-93. https://doi.org/10.2174/1874091X01408010089 
  21. Rey AI, Lopez-Bote CJ, Litta G. Effects of dietary vitamin E (DL-α-tocopheryl acetate) and vitamin C combination on piglets oxidative status and immune response at weaning. J Anim Feed Sci 2017;26:226-35. https://doi.org/10.22358/jafs/76595/2017 
  22. Moreira I, Mahan DC. Effect of dietary levels of vitamin E (all-rac-tocopheryl acetate) with or without added fat on weanling pig performance and tissue α-tocopherol concentration. J Anim Sci 2002;80:663-9. https//jas.fass.org/content/80/3/663  https://doi.org/10.2527/2002.803663x
  23. Hoppe PP. Comparison of plasma alpha- and gamma-tocopherols after oral and intramuscular administration of RRR-alpha-tocopherol or RRR-gamma-tocopherol to weanling pigs. Int J Vitam Nutr 1991;61:114-9. 
  24. Metzler-Zebeli BU, Zijlstra RT, Mosenthin R, Ganzle MG. Dietary calcium phosphate content and oat β-glucan influence gastrointestinal microbiota, butyrate-producing bacteria and butyrate fermentation in weaned pigs. FEMS Microbiol Ecol 2011;75:402-13. https://doi.org/10.1111/j.1574-6941.2010.01017.x 
  25. Metzler-Zebeli BU, Hooda S, Pieper R, et al. Nonstarch polysaccharides modulate bacterial micro biota, pathways for butyrate production, and abundance of pathogenic Escherichia coli in the pig gastrointestinal tract. Appl Env Microbiol 2010;76:3692-701. https://doi.org/10.1128/AEM.00257-10 
  26. Stuyven E, Cox E, Vancaeneghem S, Arnouts S, Deprez P, Goddeeris BM. Effect of β-glucans on an ETEC infection in piglets. Vet Immunol Immunopathol 2009;128:60-6. https://doi.org/10.1016/j.vetimm.2008.10.311 
  27. Kim K, Ehrlich A, Perng V, et al. Algae-derived β-glucan enhanced gut health and immune responses of weaned pigs experimentally infected with a pathogenic E. coli. Anim Feed Sci Technol 2019;248:114-25. https://doi.org/10.1016/j.anifeedsci.2018.12.004 
  28. Ko TG, Kim JD, Han YK, Han IK. Study for the development of antibiotics-free diet for growing pigs. Kor J Anim Sci 2000;42:45-54. 
  29. Brennan CS, Cleary LJ. The potential use of cereal (1→ 3, 1→ 4)-β-d-glucans as functional food ingredients. J Cereal Sci 2005;42:1-13. https://doi.org/10.1016/j.jcs.2005.01.002