대한본초학회지 (The Korea Journal of Herbology)

  • 제38권3호
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  • Pages.19-26
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  • 2023
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  • 1229-1765(pISSN)
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  • 2288-7199(eISSN)
대한본초학회 (The Korea Association of Herbology)
권호 표지
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용아초(龍芽草) 유기용매 분획물의 항산화 및 피부 장벽 개선 효과

Anti-oxidant Activity and Skin Barrier Function Measured in Different Solvent Fractions Obtained from Agrimoniae Herba

  • 한제근 ((주)원일바이오) ;
  • 김민주 (대구한의대학교 간질환한약융복합활용연구센터) ;
  • 박해진 (대구한의대학교 바이오융복합시험센터) ;
  • 이경하 (부산대학교 분자생물학과) ;
  • 노성수 (대구한의대학교 한의학과 본초약리학교실)
  • Jegeun Han (WONIL BIO) ;
  • Min Ju Kim (Research Center for Herbal Convergence on Liver Disease, Daegu Haany University) ;
  • Hae-Jin Park (DHU Bio Convergence Testing Center) ;
  • Kyung-Ha Lee (Department of Molecular biology, Pusan National University) ;
  • Seong-Soo Roh (Department of Herbology, College of Korean Medicine, Daegu Haany University)
  • 투고 : 2023.04.17
  • 심사 : 2023.05.25
  • 발행 : 2023.05.30
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초록

Objectives : Agrimoniae Herba is a herbal medicine widely distributed in Asia and contains flavonoids including catechin, quercitrin, rutin, hyperoside, and quercetin. This study aimed to investigate the anti-oxidant activity and skin barrier function of different solvent fractions (Hexane; methylene chloride, MC; ethyl acetate, EA; n-butanol, Bu; Water) obtained from Agrimoniae Herba. Methods : Anti-oxidant activity of different solvent fractions obtained from Agrimoniae Herba was investigated through total polyphenol contents, total flavonoid contents, 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity measurements. Then, filament aggregating protein (Filaggrin), Type I collagen, ceramide synthase (CERS) 3, and CERS4 were analyzed to evaluate the skin barrier strengthening effect of different solvent fractions obtained from Agrimoniae Herba on UVB-stimulated HaCaT cells. Results : As a result of measuring total polyphenol contents, total flavonoid contents, DPPH free radical scavenging activity, and ABTS radical scavenging activity, antioxidant activity was found to be excellent in the order of EA > Bu > MC > Hexane > Water. As a result of measuring mRNA gene expression of Type I collagen, Filaggrin, CERS3, and CERS4 after UVB-stimulated was applied to HaCaT cells treated with different solvent fractions obtained from Agrimoniae Herba, it was found to increase significantly in the Bu-treated group. Conclusion : Our findings show that the Bu sample obtained from Agrimoniae Herba has excellent anti-oxidant ability, which increases Type I collagen, Filaggrin, and ceramide synthetase in UVB-stimulated HaCaT cells to control the skin barrier improvement effect.

키워드

과제정보

이 연구는 2023년도 정부 (과학기술정보통신부)의 재원으로 한국연구재단의 지원을 받아 수행되었습니다 (No. 2018R1A5A2025272).

참고문헌

  1. Choi JH, Hwang SJ, Jeong SN, Lee YK, Jin MH, Park SG, Lee CK. Anti-aging Effect on Skin with 9 Repetitive Steaming and Fermenting Process Herbal Composition Extract. J. Herbology 2009;24(4):101-6.
  2. Kim HS. Effect of Nepeta cataria extract on the skin barrier function. Korean J. Food Preserv. 2020;27(2):242-6. doi:org/10.11002/kjfp.2020.27.2.242.
  3. Chaquour B, Seite S, Coutant K, Fourtanier A, Borel JP, Bellon G. Chronic UVB- and all-trans retinoic-acid-induced qualitative and quantitative changes in hairless mouse skin. J Photochem Photobiol B. 1995;28(2):125-35. doi:10.1016/1011-1344(94)07080-8.
  4. Jeon HY, Kim JK, Kim WG, Lee SJ. Beneficial Effect of Curcumin on Epidermal Permeability Barrier Function in Hairless Rat. KOREAN J. FOOD SCI. TECHNOL. 2008;40(6):686-90.
  5. Park CS. Skin barrier and beauty foods. Food Sci. 2008;40:19-26. doi:org/10.23093/FSI.2007.40.4.19.
  6. Choi YS, Kim YS. Neuroprotective Effects of Agrimoniae Herba against Intrastriatal Hemorrhage in Rats. Kor. J. Herbology 2010;25(4):31-7.
  7. Xu X, Qi X, Wang W, Chen G. Separation and determination of flavonoids in Agrimonia pilosa Ledeb. by capillary electrophoresis with electrochemical detection. J Sep Sci. 2005;28(7):647-52. doi:10.1002/jssc.200400095.
  8. Pan Y, Liu HX, Zhuang YL, Ding LQ, Chen LX, Qiu F. [Studies on isolation and identification of flavonoids in herbs of Agrimonia pilosa]. Zhongguo Zhong Yao Za Zhi. 2008;33(24):2925-8.
  9. Kumar A, Sehgal N, Kumar P, Padi SS, Naidu PS. Protective effect of quercetin against ICV colchicine-induced cognitive dysfunctions and oxidative damage in rats. Phytother Res. 2008;22(12):1563-9. doi: 10.1002/ptr.2454.
  10. Rezai-Zadeh K, Ehrhart J, Bai Y, Sanberg PR, Bickford P, Tan J, Shytle RD. Apigenin and luteolin modulate microglial activation via inhibition of STAT1-induced CD40 expression. J Neuroinflammation. 2008;5:41. doi:10.1186/1742-2094-5-41.
  11. Yoon JY, Lee SY, Jun HJ, Lee JY. Anti-aging Effects of Solvent Fraction from Agrimonia pilosa L. Extracts [Internet]. Vol. 55, Journal of Applied Biological Chemistry. J Appl Biol Chem 2012;55(1):35-9. doi:org/10.3839/jabc.2011.056.
  12. Kim HJ, Lim HW, Kim BH, Choi SW, Yoon CS. Studies on the Anti-acne Effect of Agrimonia pilosa Ledeb. J Soc Cosmet Scientists Korea 2006;32(1):53-8.
  13. Kang SC, Lee CM, Koo HJ, Ahn DH, Choi H, Lee JH, Bak JP, Lee MH, Choung ES, Kawk JH. Hepatoprotective effects of aqueous extract from aerial part of Agrimmony. Kor J Pharmacogn. 2006;37(1):28-32.
  14. Hua CL, Lee JK, Cho KH, Kang DG, Kwon TO, Kwon JW, Kim JS, Sohn EJ, Lee HS. Mechanism
  15. for the vascular relaxation induced by butanol extract of Agrimonia pilosa. Kor J Pharmacogn. 2006;37(2):67-73.
  16. Choi SJ, Baik JW, Park JH, Jun CY, Choi YK, Ko SG. Mechanism of apoptosis & tumor growth inhibition of Agrimonia pilosa Ladebour (APL) in vitro and in vivo. Korean J Orient Int Med. 2009;30(2):399-409.
  17. Kim JJ, Jiang J, Shim DW, Kwon SC, Kim TJ, Ye SK, Kim MK, Shin YK, Koppula S, Kang TB, Choi DK, Lee KH. Anti-inflammatory and anti-allergic effects of Agrimonia pilosa Ledeb extract on murine cell lines and OVA-induced airway inflammation. J Ethnopharmacol. 2012;140(2):213-21. doi:10.1016/j.jep.2011.12.035.
  18. Saeed N, Khan MR, Shabbir M. Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L. BMC Complement Altern Med. 2012;12:221. doi:10.1186/1472-6882-12-221.
  19. Malla MY, Sharma M, Saxena RC, Mir MI, Mir AH, Bhat SH. Phytochemical screening and spectroscopic determination of total phenolic and flavonoid contents of Eclipta Alba Linn. J. Nat. Prod. Plant Resour. 2013;3(2):86-91.
  20. Blosis MS. Antioxidant determinations by the use of a stable free radical. Nature. 1958;26:1199-200. doi:org/10.1038/1811199a0.
  21. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999;26(9-10): 1231-7. doi:10.1016/s0891-5849(98)00315-3.
  22. Ha CW, Sohn EH, Kim SH, Jang S, Park MR, Kim YK, Bae IY. Fermented Angelicae tenussimae with Aspergillus oryzae Improves Skin Barrier Properties, Moisturizing, and Anti-Inflammatory Responses. Int J Mol Sci. 2022;23(20):12072. doi:10.3390/ijms232012072.
  23. Park NJ, Jo BG, Bong SK, Park SA, Lee S, Kim YK, Yang MH, Kim SN. Lobelia chinensis Extract and Its Active Compound, Diosmetin, Improve Atopic Dermatitis by Reinforcing Skin Barrier Function through SPINK5/LEKTI Regulation. Int J Mol Sci. 2022;23(15):8687. doi: 10.3390/ijms23158687.
  24. Koo JS. A Study on the Skin Improvement Effect of Castanea crenata Inner Shell with Microneedle Therapy System. Kor. J. Herbol. 2022;37(2):23-31.
  25. Jing C, Guo J, Li Z, Xu X, Wang J, Zhai L, Liu J, Sun G, Wang F, Xu Y, Li Z, Zhao D, Jiang R, Sun L. Screening and Research on Skin Barrier Damage Protective Efficacy of Different Mannosylerythritol Lipids. Molecules. 2022;27(14):4648. doi:10.3390/molecules27144648.
  26. Baek J, Lee MG. Oxidative stress and antioxidant strategies in dermatology. Redox Rep. 2016;21(4):164-9. doi:10.1179/1351000215Y.0000000015.
  27. Yoon YM, Yoon JY, Lim KM, Hahn HJ, Kim YR, Ahn KJ, An SK. Effects of the Complex Containing Centella asiatica - and Folic Acid-Ferment Extracts, Acetyl Glutamine, and Nicotinic Acid Adenine Dinucleotide Phosphate on the Inhibition of Senescence and Melanogenesis, Promotion of Collagen Expression, Cellular Regeneration, and Keratinocyte Differentiation, and Anti-inflammation. Kor. J. Aesthet. Cosmetol. 2013;11(4): 675-84.
  28. Sandilands A, Sutherland C, Irvine AD, McLean WH. Filaggrin in the frontline: role in skin barrier function and disease. J Cell Sci. 2009;122(Pt 9):1285-94. doi:10.1242/jcs.033969.
  29. Coderch L, Lopez O, de la Maza A, Parra JL. Ceramides and skin function. Am J Clin Dermatol. 2003;4(2):107-29. doi:10.2165/00128071-200304020-00004.
  30. Schurer NY, Plewig G, Elias PM. Stratum corneum lipid function. Dermatologica. 1991;183(2):77-94. doi: 10.1159/000247644.
  31. Jungersted JM, Hellgren LI, Jemec GB, Agner T. Lipids and skin barrier function--a clinical perspective. Contact Dermatitis. 2008;58(5):255-62. doi: 10.1111/j.1600-0536.2008.01320.x.