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Copy Paper as a Platform for Low-cost Sensitive Glucose Sensing

  • Ye Lin Kim (Industry 4.0 Convergence Bionics Engineering, Pukyong National University) ;
  • Young-Mog Kim (Department of Food Science and Technology, Pukyong National University) ;
  • Junghwan Oh (Industry 4.0 Convergence Bionics Engineering, Pukyong National University) ;
  • Joong Ho Shin (Industry 4.0 Convergence Bionics Engineering, Pukyong National University)
  • Received : 2023.01.16
  • Accepted : 2023.01.29
  • Published : 2023.01.31

Abstract

This study reports the potential of using commercial copy papers as substrates for simple sensitive glucose detection. Typical paper-based devices use filter papers as porous substrates that can contain reagents; however, this is the first study to report the use of copy papers for the purpose of enhancing enzymatic colorimetric detection. Glucose detection using glucose oxidase, horseradish peroxidase and potassium iodide was performed on a copy paper, cellulose-based filter paper, and polyethylene film. The results indicated that the copy paper exhibited a stronger coloration than the other substrates. Reagents required for detection were dried on the copy paper, and a 3D-printed holder was designed to provide an environment for consistent imaging, making it a convenient cost-effective option for point-of-care testing using a mobile phone camera. The simple paper-based glucose sensor exhibited a linear range of 0.1-20 mM, limit of quantification of 0.477 mM, and limit of detection of 0.143 mM.

Keywords

Acknowledgement

This study was supported by the Pukyong National University Development Project Research Fund, 2022, and the National Research Foundation of Korea (NRF) funded by the Korean government (MSIT) (Grant Nos. 2020R1C1C1003567 and 2022R1A5A8023404).

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