Journal of the Korean Society of Food Culture (한국식생활문화학회지)

Korean Society of Food Culture (한국식생활문화학회)
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Comparative Analysis of Antioxidant Activity of Korean Seaweeds Extracts

국내 자생 해조류 추출물의 항산화능 비교분석 연구

  • Kyong Kim (Department of Food and Nutrition, Eulji University) ;
  • Kyung Ha Lee (Department of Food and Nutrition, Eulji University) ;
  • Hye Won Yang (Department of Food and Nutrition, Eulji University) ;
  • Chae Hyeon Woo (Department of Food and Nutrition, Eulji University) ;
  • Woo-Hyuk Jung (Marine Biotechnology Research Center, Jeonnam Bioindustry Foundation) ;
  • Eun-Young Park (Department of Pharmacy, Mokpo National University) ;
  • Yoon Sin Oh (Department of Food and Nutrition, Eulji University)
  • 김경 (을지대학교 식품영양학과) ;
  • 이경하 (을지대학교 식품영양학과) ;
  • 양혜원 (을지대학교 식품영양학과) ;
  • 우채현 (을지대학교 식품영양학과) ;
  • 정우혁 (전남바이오산업진흥원 해양바이오연구센터) ;
  • 박은영 (목포대학교 약학대학) ;
  • 오윤신 (을지대학교 식품영양학과)
  • Received : 2023.01.17
  • Accepted : 2023.04.03
  • Published : 2023.04.30
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Abstract

Seaweed-derived foods have long been popular in Korea because of their high content of nutrients that are beneficial to the human body. Recently, Korean seaweeds have been used as raw materials to produce new natural products with health benefits. Herein, we compared the antioxidant activity of 16 Korean seaweed extracts to explore their potential utility as health foods. The total phenolic content (TPC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of seaweed extracts were determined. We also investigated their ability to protect human diploid fibroblast (HDF) cells against hydrogen peroxide. The results showed that seaweed extracts at a concentration of 100 ㎍/mL did not cause any cell toxicity. Sargassum thunbergii (Jichung-i) had the highest TPC and radical scavenging effects, followed by Porphyra tenera (Gim), Silvetia siliquosa (Tteumbugi), and Sargassum fusiforme (Tot). Hydrogen peroxide increased the production of intracellular reactive oxygen species, while P. tenera (Gim), Saccharina japonica (Dasima), and S. thunbergii (Jichung-i) extracts significantly decreased it. The effect was highest in the S. thunbergii (Jichung-i)-treated HDF cells. These findings indicate that S. thunbergii (Jichung-i) shows promise as a potential antioxidant raw material.

Keywords

References

  1. Bayr H. 2005. Reactive oxygen species. Critical Care Medicine, 33
  2. Blois MS. 1958. Antioxidant Determinations by the Use of a Stable Free Radical. Nature, 181: 1199-1200 https://doi.org/10.1038/1811199a0
  3. Chiba K, Kawakami K, Sone T, Onoue M. 2003. Characteristics of skin wrinkling and dermal changes induced by repeated application of squalene monohydroperoxide to hairless mouse skin. Skin Pharmacol. Appl. Skin Physiol., 16: 242-51 https://doi.org/10.1159/000070847
  4. Cho KA, Ryu SJ, Oh YS, Park JH, Lee JW, Kim HP, Kim KT, Jang IS, Park SC. 2004. Morphological adjustment of senecent cells by modulating caveolin-1 status. J. Bio. Chem., 279(40):42270-42280 https://doi.org/10.1074/jbc.M402352200
  5. Choi HY, Choi NY, Son MS, Kim DS, Lee HJ. 2020. Assessment of possibility as comsmetic materials by brown algae from Jeju island using supercritical fluid system. J. Korea Acad.-Ind. Coop. Soc., 21(1): 698-704
  6. Dang TT, Bowyer MC, Van Altena IA, Scarlett CJ. 2018. Comparision of chemical profile and antioxidant properties of the brown algae. Int. J. Food Sci., 53(1): 174-181 https://doi.org/10.1111/ijfs.13571
  7. Devi GK, Manivannan K. Thirumaran G, Rajathi FAA, Anantharaman P. 2011. In vitro antioxidant activities of selected seaweeds from Southeast coast of India. Asian Pac. J. Trop. Med., 4: 205-211 https://doi.org/10.1016/S1995-7645(11)60070-9
  8. Duh P-D, Tu Y-Y, Yen G-C. 1999. Antioxidant Activity of Water Extract of Harng Jyur (Chrysanthemum morifolium Ramat). LWT - Food Sci. Tech., 32: 269-277 https://doi.org/10.1006/fstl.1999.0548
  9. El-Beltagi HS, Mohamed AA, Mohamed HI, Ramadan KMA, Barqawi AA, Mansour AT. 2022. Phytochemical and Potential Properties of Seaweeds and Their Recent Applications: A Review. Mar. Drugs, 20: 342
  10. Folin O, Denis W. 1912. On Phosphotungstic-phosphomolybdic Compounds as Color Reagents. J. Bio. Chem., 12: 239-243 https://doi.org/10.1016/S0021-9258(18)88697-5
  11. Generalic Mekinic I, Skroza D, Simat V, Hamed I, Cagalj M, Popovic Perkovic Z. 2019. Phenolic content of brown algae species: extraction, identification, and quantification. Biomol., 9(6): 244-269 https://doi.org/10.3390/biom9060244
  12. Halliday GM. 2005. Inflammation, gene mutation and photo-immunosuppression in response to UVR-induced oxidative damage contributes to photocarcinogenesis. Mutat. Res., 571: 107-20 https://doi.org/10.1016/j.mrfmmm.2004.09.013
  13. Heo SJ, Park EJ, Lee KW, Jeon YJ. 2005. Antioxidant activities of enzymatic extracts from brown seaweeds. Bioresour. Technol., 96: 1613-1623 https://doi.org/10.1016/j.biortech.2004.07.013
  14. Huang HL, Wang BG. 2004. Antioxidant capacity and lipophilic content of seaweeds collected from the Qingdao coastline. J. Agric. Food Chem., 52: 4993-7 https://doi.org/10.1021/jf049575w
  15. Jimenez-Escrig A, Goni Cambrodon I. 1999. Nutritional evaluation and physiological effects of edible seaweeds. Arch. Latinoam. Nutr., 49: 114-20
  16. Kang JY, Khan MN, Park NH, Cho JY, Lee MC, Fujii H, Hong YK. 2008. Antipyretic, analgesic, and anti-inflammatory activities of the seaweed Sargassum fulvellum and Sargassum thunbergii in mice. J. Ethnopharmacol., 116: 187-90 https://doi.org/10.1016/j.jep.2007.10.032
  17. Kumari P, Redd RCK, Jha B. 2011. Comparative evaluation and selection of a method for lipid and fatty acid extraction from macroalgae. Anal. Biochem., 415(2): 134-144 https://doi.org/10.1016/j.ab.2011.04.010
  18. Larson RA. 1988. The antioxidants of higher plants. Phytochem., 27: 969-978 https://doi.org/10.1016/0031-9422(88)80254-1
  19. Lee NY. 2013. Antioxidant Effect and Tyrosinase Inhibition Activity of Seaweeds Ethanol Extracts. J. Korean Soc. Food. Sci. Nutr., 42: 1893-1898 https://doi.org/10.3746/jkfn.2013.42.12.1893
  20. Lim SN, Cheung PC, Ooi VE, Ang PO. 2002. Evaluation of antioxidative activity of extracts from a brown seaweed, Sargassum siliquastrum. J. Agric. Food Chem., 50: 3862-6 https://doi.org/10.1021/jf020096b
  21. Liu Z, Ma LP, Zhou B, Yang L, Liu ZL. 2000. Antioxidative effects of green tea polyphenols on free radical initiated and photosensitized peroxidation of human low density lipoprotein. Chem. Phys. Lipids, 106: 53-63 https://doi.org/10.1016/S0009-3084(00)00133-X
  22. Meenakshi S, Umayaparvathi S, Arumugam M, Balasubramanian T. 2012. In vitro antioxidant properties and FTIR analysis of two seaweeds of Gulf of Mannar. Asian Pac. J. Trop. Med., S66-S70
  23. Osborn-Barnes HT, Akoh CC. 2003. Effects of alpha-tocopherol, beta-carotene, and soy isoflavones on lipid oxidation of structured lipid-based emulsions. J. Agric. Food Chem., 51: 6856-60 https://doi.org/10.1021/jf026212s
  24. Park KE, Kim YA, Jung HA, Lee HJ, Ahn JW, Lee BJ, Seo YW. 2004. Three Norisoprenoids from the Brown Alga Sargassum thunbergii. J. Korean Chem. Soc., 48
  25. Park PJ, Heo SJ, Park EJ, Kim SK, Byun HG, Jeon BT, Jeon YJ. 2005. Reactive Oxygen Scavenging Effect of Enzymatic Extracts from Sargassum thunbergii. J. Agric. Food Chem., 53: 6666-6672 https://doi.org/10.1021/jf050582+
  26. Priya RR, Khora SS. 2023. Antioxidant potentials of polysaccharides derived from marine brown algae. Mar. Antioxidants, 33:433-448 https://doi.org/10.1016/B978-0-323-95086-2.00012-6
  27. Rath B. 2012. Commercial and industrial applications of micro algae - A review. J. Algal. Biomass. Utln., 3: 89-100
  28. Rosenkranz AR, Schmaldienst S, Stuhlmeier KM, Chen W, Knapp W, Zlabinger GJ. 1992. A microplate assay for the detection of oxidative products using 2',7'-dichlorofluorescin-diacetate. J. Immunol. Methods, 156: 39-45 https://doi.org/10.1016/0022-1759(92)90008-H
  29. Shin SH, Kang SM. 2021. The Antioxidation Effect of Brown Algae Extract. J. Korean Soc. Cosmetol., 27: 851-858 https://doi.org/10.52660/JKSC.2021.27.4.851
  30. Tedesco I, Russo M, Russo P, Iacomino G, Russo GL, Carraturo A, Faruolo C, Moio L, Palumbo R. 2000. Antioxidant effect of red wine polyphenols on red blood cells. J. Nutr. Biochem., 11: 114-9 https://doi.org/10.1016/S0955-2863(99)00080-7
  31. Vistica VT, Skehan P, Scudiero D, Monks A, Pittman A, Boyd MR. 1991. Tetrazolium-based assays for cellular viability: a critical examination of selected parameters affecting formazan production. Cancer Res., 51: 2515-20
  32. Yan X, Nagata T, Fan X. 1998. Antioxidative activities in some common seaweeds. Plant Foods Hum. Nutr., 52: 253-62 https://doi.org/10.1023/A:1008007014659
  33. Yen GC, Hsieh CL. 1998. Antioxidant activity of extracts from du-zhong (Eucommia ulmoides) toward various lipid peroxidation models in vitro. J. Agric. Food Chem., 46: 3952-3957 https://doi.org/10.1021/jf9800458
  34. Yi Z, Yin-shan C, Hai-sheng L. 2001. Screening for antibacterial and antifungal activities in some marine algae from the Fujian coast of China with three different solvents. Chin. J. Oceanol. Limnol., 19: 327-331 https://doi.org/10.1007/BF02850736