The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
권호 표지
DOI QR코드

DOI QR Code

Validation of Asiaticoside as Marker Compound of Centella asiatica Juice and Extract, and Its Antioxidant Activity

병풀(Centella asiatica) 착즙액과 추출물의 Asiaticoside 분석법 검증 및 항산화 활성

  • Yeon Suk Kim (Major in Food and Nutrition, Korea National University of Transportation) ;
  • Hyun Young Shin (Transdisciplinary Major in Learning Health System, Dept. of Integrated Biomedical & Life Science, Korea University) ;
  • Eun Ji Ha (Major in Food and Nutrition, Korea National University of Transportation) ;
  • Ja Pyeong Koo (Major in Food and Nutrition, Korea National University of Transportation) ;
  • Se Bin Jeong (Major in Food and Nutrition, Korea National University of Transportation) ;
  • Gaeuleh Kim (Major in Food and Nutrition, Korea National University of Transportation) ;
  • Mi Yeun Joung (Corporation ChamSunJin Green Juice) ;
  • Kwang-Won Yu (Major in Food and Nutrition, Korea National University of Transportation)
  • 김연숙 (한국교통대학교 식품영양학전공) ;
  • 신현영 (고려대학교 대학원 의생명융합과학과 러닝헬스시스템 융합전공) ;
  • 하은지 (한국교통대학교 식품영양학전공) ;
  • 구자평 (한국교통대학교 식품영양학전공) ;
  • 정세빈 (한국교통대학교 식품영양학전공) ;
  • 김가을에 (한국교통대학교 식품영양학전공) ;
  • 정미연 ((주)참선진녹즙) ;
  • 유광원 (한국교통대학교 식품영양학전공)
  • Received : 2023.01.13
  • Accepted : 2023.02.24
  • Published : 2023.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

Centella asiatica (C. asiatica) has been widely used in food, cosmetics, and pharmaceutical industry as a functional material. In a previous study, we have investigated not only pharmacological effects such as antioxidative and anti-inflammatory effects, but also analyzed various functional ingredients. In this study, triterpenoids were analyzed using HPLC-DAD to determine marker compounds among functional ingredients. When triterpenoids were analyzed, asiaticoside from C. asiatica was determined as an optimal marker compound. Next, specificity, linearity, limited of detection (LOD), limited of quantification (LOQ), precision, accuracy, and range were evaluated using HPLC-DAD to determine asiaticoside contents in C. asiatica juice and extracts. The specificity was elucidated by chromatogram and retention time using an established analytical method. The coefficient of correlation obtained was 0.9996. LOD was 4.99 ㎍/mL and LOQ was 15.12 ㎍/mL. Intra- and inter-day precision of asiaticoside were determined to be 0.48~1.68% and 0.08~1.09%, respectively. Furthermore, the recovery rate of asiaticoside was 98.88% and the analytical range of Field-70E was determined to be 0.625~10 mg/mL. As a results of evaluating ABTS, DPPH, and FRAP antioxidative effect, Field-70E showed potent antioxidant activities. Results of this study could be used as basic data for quality standardization of C. astiatica juice and extracts.

Keywords

Acknowledgement

본 논문은 2022년도 교육부의 재원으로 한국연구재단의 지원을 받아 수행된 지자체-대학 협력기반 지역혁신 사업의 결과입니다(2021RIS-001; 핵심기술개발사업).

References

  1. Benzie IFF, Strain JJ. 1996. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": The FRAP assay. Anal Biochem 239:70-76 https://doi.org/10.1006/abio.1996.0292
  2. Bian D, Liu M, Li Y, Xia Y, Gong Z, Dai Y. 2012. Madecassoside, a triterpenoid saponin isolated from Centella asiatica herbs, protects endothelial cells against oxidative stress. J Biochem Mol Toxicol 26:399-406 https://doi.org/10.1002/jbt.21434
  3. Bylka W, Znajdek-Awizen P, Studzinska-Sroka E, Brzezinska M. 2013. Centella asiatica in cosmetology. Postepy Dermatol Alergol 30:46-49 https://doi.org/10.5114/pdia.2013.33378
  4. Choi EY, Heo SI, Kwon YS, Kim MJ. 2016. Anti-oxidant activity and anti-inflammatory effects of Spiraea fritschiana Schneid extract. Korean J Med Crop Sci 24:31-37 https://doi.org/10.7783/KJMCS.2016.24.1.31
  5. Choi HM, Kim SG, Kim HY, Woo SO, Kim S, Moon HJ, Han SM. 2022. Determination of spermidine in freeze-dried royal jelly using ultra performance liquid chromatography. J Apic 37:51-57 https://doi.org/10.17519/apiculture.2022.04.37.1.51
  6. Choi JN, Lee HJ, Lee YJ, Jeong JT, Lee JH, Chang JK, Park CG. 2020. Growth characteristics and asiaticoside content, and antioxidant activities in Centella asiatica by cultivation and irrigation methods. Korean J Med Crop Sci 28:254-259 https://doi.org/10.7783/KJMCS.2020.28.4.254
  7. Choi JN, Oh MW, Lee HJ, Lee JH, Jeong JT, Lee YJ, Chang JK, Park CG. 2021a. Comparison of growth characterisitics, asiaticoside content and antioxidant activities of Centella asiatica (L.) Urb. Korean J Plant Resour 34:44-51
  8. Choi SI, Han X, Men X, Lee SJ, Park MH, Lee OH, Yang JM, Choi YE, Cho JH. 2021b. Development and validation of an analytical method for 2-furoic acid in Benincasa hispida extracts (HR1901-W). J Agric Life Environ Sci 33:311-320
  9. Eom HJ, Shin HY, Ji YM, Kwon NR, Yoon HS, Kim IJ, Song Y, Yu KW. 2021. Nutritional components and physiological activity of Centella asiatica cultured in Chungju by drying method. Korean J Food Nutr 34:165-173
  10. Eom HJ, Shin HY, Park HJ, Kim KH, Kim JH, Yu KW. 2022. Functional components and physiological activity in different parts of Centella asiatica. Korean J Food Preserv 29:749-761 https://doi.org/10.11002/kjfp.2022.29.5.749
  11. Ha JH, Kwon MC, Kim Y, Jeong SS, Jeong MH, Hwang B, Lee HY. 2009. Enhancement of immuno-modulatory of Centella asiatica L. Urban with edible polymer through nano-encapsulation process. Korean J Med Crop Sci 17:257-265
  12. Han X, Choi SI, Men X, Lee S, Oh G, Jin H, Oh HJ, Kim E, Kim J, Lee BY, Lee OH. 2022. Modification and validation of an analytical method for dieckol in Ecklonia stolonifera extract. J Food Hyg Saf 37:143-148 https://doi.org/10.13103/JFHS.2022.37.3.143
  13. Hong JH, Lee MJ, Jo YI, Moon SM, Lee SA, Kim CS. 2021. Analytical method validation of cynaroside in domestic Anthriscus sylvestris (L.) Hoffm. leaves extract for standardization as a functional ingredient using RP-HPLC. J Korean Soc Food Sci Nutr 50:395-402 https://doi.org/10.3746/jkfn.2021.50.4.395
  14. Kil YS, Sin SM, Lee DY, Min JW, Yang K, Lee SW, Kim YH, Goo YM. 2018. Analysis of triterpene glycoside levels and antioxidant activity in the different shoot tissues of Centella asiatica (L.) Urban. J Life Sci 28:917-922
  15. Kwon JG, Jung YW, Choi YH, Lee JE, Jeong W, Lee JA, Choi CW, Ahn EK, Choi Y, Hong SS. 2022. Development and validation of an analytical method for the detection of resveratrol and trans-scirpusin A as functional ingredients Smilax sieboldii extract. J Korean Soc Food Sci Nutr 51:1171-1177 https://doi.org/10.3746/jkfn.2022.51.11.1171
  16. Kwon KJ, Choi SJ, Yoon Y. 2012. Improving effects of the cleanser containing Centella asiatica extracts and charcoal and pearl powder on the facial skin. Korean J Aesthet Cosmetol 10:581-586
  17. Lee JH, Jeong KO, Im SY, Lee SJ. 2021. Analytical method validation of ellagic acid as an antioxidative marker compound of the Rubus occidentalis extract. Korean J Food Preserv 28:663-673 https://doi.org/10.11002/kjfp.2021.28.5.663
  18. Li H, Gong X, Zhang L, Zhang Z, Luo F, Zhou Q, Chen J, Wan J. 2009. Madecassoside attenuates inflammatory response on collagen-induced arthritis in DBA/1 mice. Phytomedicine16:538-546
  19. Liu M, Dai Y, Li Y, Luo Y, Huang F, Gong Z, Meng Q. 2008. Madecassoside isolated from Centella asiatica herbs facilitates burn wound healing in mice. Planta Med 74:809-815 https://doi.org/10.1055/s-2008-1074533
  20. National Institute of Food and Drugs Safety Evaluation [NIFDS]. 2021. Guide to preparation of submission materials for recognition of functional ingredients for health functional food. pp.42-49. National Institute of Food and Drugs Safety Evaluation. Report No. 0769-04
  21. Oh S, Park S, Lee S, Park Y, Jang KI, Yu KW, Kim D, Shin H. 2021. Comparison of growth characteristics and physiological activity of two Centella asiatica cultivars in green-house soil culture. J Bio-Environ Control 30:351-358 https://doi.org/10.12791/KSBEC.2021.30.4.351
  22. Park DW, Jeon H, So R, Kang SC. 2020. Centella asiatica extract prevents visual impairment by promoting the production of rhodopsin in the retina. Nutr Res Pract 14:203-217 https://doi.org/10.4162/nrp.2020.14.3.203
  23. Shin HY, Kim H, Jeong EJ, Kim JE, Lee KH, Bae YJ, Yu KW. 2020. Bioactive compounds, anti-oxidant activities and anti-inflammatory activities of solvent extracts from Centella asiatica cultured in Chungju. Korean J Food Nutr 33:692-701
  24. Shin HY, Kim H, Jung S, Jeong EJ, Lee KH, Bae YJ, Suh HJ, Jang KI, Yu KW. 2021. Interrelationship between secondary metabolites and antioxidant capacities of Centella asiatica using bivariate and multivariate correlation analyses. J Appl Biol Chem 64:82