한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제51권1호
  • /
  • Pages.59-68
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  • 2023
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  • 1598-642X(pISSN)
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  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Assessment and Optimization of Xylanase Production Using Mono-Culture and Co-Cultures of Bacillus subtilis and Bacillus pumilus

  • Chitranshu Pandey (Faculty of Biosciences, Institute of Biosciences and Technology, Shri Ramswaroop Memorial University) ;
  • Neeraj Gupta (Faculty of Biosciences, Institute of Biosciences and Technology, Shri Ramswaroop Memorial University)
  • 투고 : 2023.01.02
  • 심사 : 2023.01.16
  • 발행 : 2023.03.28
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초록

Xylanase is an industrially relevant enzyme used for the production of xylobiose and xylose. Various methods are used to enhance the microbial yield of xylanase. In the present study, co-culturing of Bacillus subtilis and Bacillus pumilus were investigated using submerged fermentation for xylanase production, which was markedly increased when sal, sagwan, newspaper, wheat bran, and xylan were used as single carbon sources. Maximum xylanase production was reported after 5 days of incubation in optimized media at pH 7.0 and 37℃, resulting in 2.69 ± 0.25 µmol/min by coculture. The 1:1 ratio of sal and sagwan in optimized production media was shown to be suitable for xylanase synthesis in submerged fermentation (SMF). In comparison to mono-culture using B. pumilus and B. subtilis, co-culturing resulted in an overall 3.8-fold and 2.15-fold increase in xylanase production, respectively.

키워드

과제정보

Mr. Manoj Verma, Director, MRD LifeSciences Pvt. Ltd., Lucknow, deserves my heartfelt gratitude. I am grateful to Dr. Pallavi Sharma (Research Scientist MRDLS, Lucknow) for her invaluable assistance during the review process, and I am also grateful to the almighty, without whose blessing nothing would have been possible.

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