한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제51권1호
  • /
  • Pages.18-25
  • /
  • 2023
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  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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The Mutant Lactobacillus plantarum GNS300 Showed Improved Exopolysaccharide Production and Antioxidant Activity

  • Jae-Youn Jung (Department of Biohealth-Machinery Convergence Engineering, Kangwon National University) ;
  • Deok-Ho Kwon (Department of Biohealth-Machinery Convergence Engineering, Kangwon National University) ;
  • Yoo Jin Lee (Department of Bioengineering and Technology, Kangwon National University) ;
  • Young Keun Song (The Garden of Naturalsolution Co., Ltd) ;
  • Moon Sik Chang (The Garden of Naturalsolution Co., Ltd) ;
  • Suk-Jin Ha (Department of Biohealth-Machinery Convergence Engineering, Kangwon National University)
  • 투고 : 2022.11.28
  • 심사 : 2023.02.22
  • 발행 : 2023.03.28
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초록

After random mutagenesis, the mutant Lactobacillus plantarum GNS300 showed improved exopolysaccharide production as determined by the quantification of total sugar. The mutant L. plantarum GNS300 produced 2.82 g/l of exopolysaccharide which showed 79.62% improved exopolysaccharide production compared with the parental strain. When exopolysaccharide of L. plantarum GNS300 was analyzed, the exopolysaccharide is composed of galactose (93.35%) and glucose (6.65%). Through the optimization of fermentation conditions using a bioreactor, 2.93 g/l of exopolysaccharide was produced from 20 g/l of glucose at 35℃, 500 rpm, and 0.1 vvm for 12 h. The mutant L. plantarum GNS300 exhibited 69.18% higher antioxidant activity than that from the parental strain, which might be caused by higher exopolysaccharide production. The concentrated supernatant of the mutant L. plantarum GNS300 inhibited the growth of gram-positive bacteria (Bacillus cereus and Staphylococcus aureus) and gram-negative bacteria (Escherichia coli, Vibrio parahaemolyticus, and Salmonella typhimurium).

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과제정보

This research was supported by research program funded by The Garden of Naturalsolution Co., Ltd (C1016186-01-01).

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