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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Preventive effects of nano-graphene oxide against Parkinson's disease via reactive oxygen species scavenging and anti-inflammation

  • Hee-Yeong Kim (Adult Stem Cell Research Center and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University) ;
  • Hyung Ho Yoon (Department of Neurological Surgery, Asan Medical Center, University of Ulsan College of Medicine) ;
  • Hanyu Seong (Department of Neurosurgery, Seoul Bumin Hospital) ;
  • Dong Kwang Seo (Department of Neurological Surgery, Asan Medical Center, University of Ulsan College of Medicine) ;
  • Soon Won Choi (Adult Stem Cell Research Center and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University) ;
  • Jaechul Ryu (Institute of Bio & Nano Convergence, Biogo Co., Ltd.) ;
  • Kyung-Sun Kang (Adult Stem Cell Research Center and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University) ;
  • Sang Ryong Jeon (Department of Neurological Surgery, Asan Medical Center, University of Ulsan College of Medicine)
  • Received : 2022.09.08
  • Accepted : 2022.11.23
  • Published : 2023.03.31
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Abstract

We investigated the neuroprotective effects of deca nano-graphene oxide (daNGO) against reactive oxygen species (ROS) and inflammation in the human neuroblastoma cell line SH-SY5Y and in the 6-hydroxydopamine (6-OHDA) induced Parkinsonian rat model. An MTT assay was performed to measure cell viability in vitro in the presence of 6-OHDA and/or daNGO. The intracellular ROS level was quantified using 2',7'-dichlorofluorescein diacetate. daNGO showed neuroprotective effects against 6-OHDA-induced toxicity and also displayed ROS scavenging properties. We then tested the protective effects of daNGO against 6-OHDA induced toxicity in a rat model. Stepping tests showed that the akinesia symptoms were improved in the daNGO group compared to the control group. Moreover, in an apomorphine-induced rotation test, the number of net contralateral rotations was decreased in the daNGO group compared to the control group. By immunofluorescent staining, the animals in the daNGO group had more tyrosine hydroxylase-positive cells than the controls. By anti-Iba1 staining, 6-OHDA induced microglial activation showed a significantly decrease in the daNGO group, indicating that the neuroprotective effects of graphene resulted from anti-inflammation. In conclusion, nano-graphene oxide has neuroprotective effects against the neurotoxin induced by 6-OHDA on dopaminergic neurons.

Keywords

Acknowledgement

This study was supported by an Asan Institute for Life Sciences Grant (2022IP0076, 2022IL0035) from Asan Medical Center, Seoul, Republic of Korea, and by the Basic Science Research Program through a National Research Foundation of Korea (NRF) grant of the Korean government (MSIT) (No. 2020R1A4A4078907).

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