Journal of Veterinary Science

The Korean Society of Veterinary Science (대한수의학회)
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HPAI-resistant Ri chickens exhibit elevated antiviral immune-related gene expression

  • Thi Hao Vu (Department of Animal Science and Technology, Chung-Ang University) ;
  • Jubi Heo (Department of Animal Science and Technology, Chung-Ang University) ;
  • Yeojin Hong (Department of Animal Science and Technology, Chung-Ang University) ;
  • Suyeon Kang (Department of Animal Science and Technology, Chung-Ang University) ;
  • Ha Thi Thanh Tran (Department of Biochemistry and Immunology, National Institute of Veterinary Research) ;
  • Hoang Vu Dang (Department of Biochemistry and Immunology, National Institute of Veterinary Research) ;
  • Anh Duc Truong (Department of Biochemistry and Immunology, National Institute of Veterinary Research) ;
  • Yeong Ho Hong (Department of Animal Science and Technology, Chung-Ang University)
  • Received : 2022.09.16
  • Accepted : 2022.12.05
  • Published : 2023.01.31
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Abstract

Background: Highly pathogenic avian influenza viruses (HPAIVs) is an extremely contagious and high mortality rates in chickens resulting in substantial economic impact on the poultry sector. Therefore, it is necessary to elucidate the pathogenic mechanism of HPAIV for infection control. Objective: Gene set enrichment analysis (GSEA) can effectively avoid the limitations of subjective screening for differential gene expression. Therefore, we performed GSEA to compare HPAI-infected resistant and susceptible Ri chicken lines. Methods: The Ri chickens Mx(A)/BF2(B21) were chosen as resistant, and the chickens Mx(G)/BF2(B13) were selected as susceptible by genotyping the Mx and BF2 genes. The tracheal tissues of HPAIV H5N1 infected chickens were collected for RNA sequencing followed by GSEA analysis to define gene subsets to elucidate the sequencing results. Results: We identified four differentially expressed pathways, which were immune-related pathways with a total of 78 genes. The expression levels of cytokines (IL-1β, IL-6, IL-12), chemokines (CCL4 and CCL5), type interferons and their receptors (IFN-β, IFNAR1, IFNAR2, and IFNGR1), Jak-STAT signaling pathway genes (STAT1, STAT2, and JAK1), MHC class I and II and their co-stimulatory molecules (CD80, CD86, CD40, DMB2, BLB2, and B2M), and interferon stimulated genes (EIF2AK2 and EIF2AK1) in resistant chickens were higher than those in susceptible chickens. Conclusions: Resistant Ri chickens exhibit a stronger antiviral response to HPAIV H5N1 compared with susceptible chickens. Our findings provide insights into the immune responses of genetically disparate chickens against HPAIV.

Keywords

Acknowledgement

This research was supported by the "Cooperative Research Program for Agriculture Science and Technology Development" (Project No. PJ015612), Rural Development Administration, and Chung-Ang University Graduate Research Scholarship in 2021, Republic of Korea.

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