Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Comprehensive analysis of miRNAs, lncRNAs and mRNAs profiles in backfat tissue between Daweizi and Yorkshire pigs

  • Chen Chen (Hunan Institute of Animal and Veterinary Science) ;
  • Yitong Chang (College of Animal Science and Technology, Hunan Agricultural University) ;
  • Yuan Deng (Hunan Institute of Animal and Veterinary Science) ;
  • Qingming Cui (Hunan Institute of Animal and Veterinary Science) ;
  • Yingying Liu (Hunan Institute of Animal and Veterinary Science) ;
  • Huali Li (Hunan Institute of Animal and Veterinary Science) ;
  • Huibo Ren (Hunan Institute of Animal and Veterinary Science) ;
  • Ji Zhu (Hunan Institute of Animal and Veterinary Science) ;
  • Qi Liu (Hunan Tianfu Ecological Agricultural Limited Company) ;
  • Yinglin Peng (Hunan Institute of Animal and Veterinary Science)
  • Received : 2022.04.24
  • Accepted : 2022.09.11
  • Published : 2023.03.01
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Abstract

Objective: Daweizi (DWZ) is a famous indigenous pig breed in China and characterized by tender meat and high fat percentage. However, the expression profiles and functions of transcripts in DWZ pigs is still in infancy. The object of this study was to depict the transcript profiles in DWZ pigs and screen the potential pathway influence adipogenesis and fat deposition, Methods: Histological analysis of backfat tissue was firstly performed between DWZ and lean-type Yorkshire pigs, and then RNA sequencing technology was utilized to explore miRNAs, lncRNAs and mRNAs profiles in backfat tissue. 18 differentially expressed (DE) transcripts were randomly selected for quantitative real-time polymerase chain reaction (QPCR) to validate the reliability of the sequencing results. Finally, gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis were conducted to investigate the potential pathways influence adipocyte differentiation, adipogenesis and lipid metabolism, and a schematic model was further proposed. Results: A total of 1,625 differentially expressed transcripts were identified in DWZ pigs, including 27 upregulated and 45 downregulated miRNAs, 64 upregulated and 119 down-regulated lncRNA, 814 upregulated and 556 downregulated mRNAs. QPCR analysis exhibited strong consistency with the sequencing data. GO and KEGG analysis elucidated that the differentially expressed transcripts were mainly associated with cell growth and death, signal transduction, peroxisome proliferator-activated receptors (PPAR), AMP-activated protein kinase (AMPK), PI3K-Akt, adipocytokine and foxo signaling pathways, all of which are strongly involved in cell development, lipid metabolism and adipogenesis. Further analysis indicated that the BGIR9823_87926/miR-194a-5p/AQP7 network may be effective in the process of adipocyte differentiation or adipogenesis. Conclusion: Our study provides comprehensive insights into the regulatory network of backfat deposition and lipid metabolism in pigs from the point of view of miRNAs, lncRNAs and mRNAs.

Keywords

Acknowledgement

This work was supported by the Natural Science Foundation of Hunan Province (2021JJ30386), Innovation Platform and Talent Plan Program of Hunan Province (2021NK1009), Key Research and Development Program of Hunan Province (2020NK2024), Open Research Program of Hunan Provincial Key Laboratory (2017TP1030), Changsha·China Longping Seed Industry Silicon Valley Program (2020) and Modern Swine Industry Technology System of Hunan Province.

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