Journal of Plant Biotechnology

  • Volume 50
  • /
  • Pages.207-214
  • /
  • 2023
  • /
  • 1229-2818(pISSN)
  • /
  • 2384-1397(eISSN)
The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Analysis of the genetic diversity and population structure of Lindera obtusiloba (Lauraceae), a dioecious tree in Korea

  • Ho Bang Kim (Life Sciences Research Institute, Biomedic Co., Ltd.) ;
  • Hye-Young Lee (Life Sciences Research Institute, Biomedic Co., Ltd.) ;
  • Mi Sun Lee (Department of Industrial Plant Science and Technology, Chungbuk National University) ;
  • Yi Lee (Department of Industrial Plant Science and Technology, Chungbuk National University) ;
  • Youngtae Choi (Division of Forest Diversity, Korea National Arboretum) ;
  • Sung-Yeol Kim (Enfield Co., Chungnam National University) ;
  • Jaeyong Choi (Department of Environment and Forest Resources, Chungnam National University)
  • Received : 2023.09.08
  • Accepted : 2023.10.30
  • Published : 2023.11.13
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Abstract

Lindera obtusiloba (Lauraceae) is a dioecious tree that is widely distributed in the low-altitude montane forests of East Asia, including Korea. Despite its various pharmacological properties and ornamental value, the genetic diversity and population structure of this species in Korea have not been explored. In this study, we selected 6 nuclear and 6 chloroplast microsatellite markers with polymorphism or clean cross-amplification and used these markers to perform genetic diversity and population structure analyses of L. obtusiloba samples collected from 20 geographical regions. Using these 12 markers, we identified a total of 44 alleles, ranging from 1 to 8 per locus, and the average observed and expected heterozygosity values were 0.11 and 0.44, respectively. The average polymorphism information content was 0.39. Genetic relationship and population structure analyses revealed that the natural L. obtusiloba population in Korea is composed of 2 clusters, possibly due to two different plastid genotypes. The same clustering patterns have also been observed in Lindera species in mainland China and Japan.

Keywords

Acknowledgement

This study was carried out with the support of R&D Program for Forest Science Technology (Project No. 2022462A00-2324-0201) provided by Korea Forest Service (Korea Forestry Promotion Institute). The authors would like to thank Dr. Jinsu Gil for his helpful advice on the population structure analysis.

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