Journal of Plant Biotechnology

  • Volume 50
  • /
  • Pages.115-126
  • /
  • 2023
  • /
  • 1229-2818(pISSN)
  • /
  • 2384-1397(eISSN)
The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Biomass partitioning and physiological responses of four Moroccan barley varieties subjected to salt stress in a hydroponic system

  • Said Bouhraoua (Natural Resources and Environment Laboratory, Multidisciplinary Faculty of Taza, Sidi Mohammed Ben Abdellah University) ;
  • Mohamed Ferioun (Natural Resources and Environment Laboratory, Multidisciplinary Faculty of Taza, Sidi Mohammed Ben Abdellah University) ;
  • Srhiouar Nassira (Natural Resources and Environment Laboratory, Multidisciplinary Faculty of Taza, Sidi Mohammed Ben Abdellah University) ;
  • Abdelali Boussakouran (Natural Resources and Environment Laboratory, Multidisciplinary Faculty of Taza, Sidi Mohammed Ben Abdellah University) ;
  • Mohamed Akhazzane (Sidi Mohammed Ben Abdellah University) ;
  • Douae Belahcen (Natural Resources and Environment Laboratory, Multidisciplinary Faculty of Taza, Sidi Mohammed Ben Abdellah University) ;
  • Khalil Hammani (Natural Resources and Environment Laboratory, Multidisciplinary Faculty of Taza, Sidi Mohammed Ben Abdellah University) ;
  • Said Louahlia (Natural Resources and Environment Laboratory, Multidisciplinary Faculty of Taza, Sidi Mohammed Ben Abdellah University)
  • Received : 2023.03.27
  • Accepted : 2023.04.20
  • Published : 2023.06.13
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Abstract

A hydroponics experiment was performed to study the physiological and biochemical changes in Moroccan barley (Hordeum vulgare L.) varieties cultivated under salt stress conditions. Four barley varieties were grown under exposure to three salt concentrations, including 0, 200, and 300 mM NaCl. The ANOVA for both salt stress-sensitive and resistant varieties indicated that salt treatment represented the main source of variability in all studied traits. Salt treatment significantly reduced root and shoot dry weight (RDW and SDW), relative water content (RWC), and chlorophyll content (Chl a, Chl b, and Chl T). However, increases in electrolyte leakage (EL) along with proline and total soluble sugar (TSS) contents were recorded. In addition, large variations in all measured traits were found between varieties. The 'Massine' and 'Laanaceur' varieties displayed relatively higher RDW and SDW values. The 'Amira' and 'Adrar' varieties showed lower RWC values and Chl contents than those of the controls indicating their relative sensitivity to salt stress. Principal component analysis revealed that most of the variation was captured by PC1 (72% of the total variance) which grouped samples into three categories according to salt treatment. Correlation analyses highlighted significant associations between most parameters. Positive relationships were found between RDW, SDW, RWC, Chl content, and soluble proteins contents, while all of these parameters were negatively associated with EL intensity, proline content, and TSS content. The results from this study showed that the 'Massine' and 'Laanaceur' varieties were relatively salt-tolerant. These two salt-tolerant varieties present a good genetic background for breeding of barley varieties showing high salt tolerance.

Keywords

Acknowledgement

We would like to thank Dr. A. JILAL from the National Institute of Agricultural Research (INRA) in Morocco for providing us the seeds of barley investigated in this work. We also thank all members of the plant research station at the NRE Lab. for their helping with plant harvest and managing plants culture in the greenhouse.

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