Journal of Plant Biotechnology

  • Volume 50
  • /
  • Pages.1-10
  • /
  • 2023
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Comparative proteome profiling in the storage root of sweet potato during curing-mediated wound healing

큐어링 후 저장에 따른 고구마 저장뿌리 단백질체의 비교분석

  • Ho Yong Shin (Department of Plant Medicine, College of Agriculture and Life Sciences, IALS, Gyeongsang National University) ;
  • Chang Yoon Ji (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Ho Soo Kim (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Jung-Sung Chung (Department of Agronomy, College of Agriculture and Life Sciences, Gyeongsang National University, IALS) ;
  • Sung Hwan Choi (Division of Horticulture Science, College of Agriculture and Life Sciences, Gyeongsang National University) ;
  • Sang-Soo Kwak (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Yun-Hee Kim (Department of Biology Education, College of Education, IALS, Gyeongsang National University) ;
  • Jeung Joo Lee (Department of Plant Medicine, College of Agriculture and Life Sciences, IALS, Gyeongsang National University)
  • 신호용 (경상국립대학교 농업생명과학대학 식물의학과(농업생명과학연구원)) ;
  • 지창윤 (한국생명공학연구원 식물시스템공학연구센터) ;
  • 김호수 (한국생명공학연구원 식물시스템공학연구센터) ;
  • 정정성 (경상국립대학교 농업생명과학대학 농학과(농업생명과학연구원)) ;
  • 최성환 (경상국립대학교 농업생명과학대학 원예과학부(농업생명과학연구원)) ;
  • 곽상수 (한국생명공학연구원 식물시스템공학연구센터) ;
  • 김윤희 (경상국립대학교 사범대학 생물교육과(농업생명과학연구원)) ;
  • 이증주 (경상국립대학교 농업생명과학대학 식물의학과(농업생명과학연구원))
  • Received : 2023.02.07
  • Accepted : 2023.02.25
  • Published : 2023.03.16
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Abstract

Sweet potato (Ipomoea batatas L. Lam) is an economically important root crop and a valuable source of nutrients, processed foods, animal feeds, and pigment materials. However, during post-harvest storage, storage roots of sweet potatoes are susceptible to decay caused by various microorganisms and diseases. Post-harvest curing is the most effective means of healing wounds and preventing spoilage by microorganisms during storage. In this study, we aimed to identify proteins involved in the molecular mechanisms related to curing and study proteomic changes during the post-curing storage period. For this purpose, changes in protein spots were analyzed through 2D-electrophoresis after treatment at 33℃ (curing) and 15℃ (control) for three days, followed by a storage period of eight weeks. As a result, we observed 31 differentially expressed protein spots between curing and control groups, among which 15 were identified. Among the identified proteins, the expression level of 'alpha-amylase (spot 1)' increased only after the curing treatment, whereas the expression levels of 'probable aldo-keto reductase 2-like (spot 3)' and 'hypothetical protein CHGG_01724 (spot 4)' increased in both the curing and control groups. However, the expression level of 'sporamin A (spot 10)' decreased in both the curing and control treatments. In the control treatment, the expression level of 'enolase (spot 14)' increased, but the expression levels of 'chain A of actinidin-E-64 complex+ (spot 19)', 'ascorbate peroxidase (spot 22)', and several 'sporamin proteins (spot 20, 21, 23, 24, 27, 29, 30, and 31)' decreased. These results are expected to help identify proteins related to the curing process in sweet potato storage roots, understand the mechanisms related to disease resistance during post-harvest storage, and derive candidate genes to develop new varieties with improved low-temperature storage capabilities in the future.

고구마(Ipomoea batatas L. Lam)는 영양소, 가공 식품, 동물 사료 및 색소 재료의 유용한 공급원으로 이용 가능한 경제적으로 중요한 대표적인 뿌리 작물이다. 일반적으로 고구마의 저장 뿌리는 수확 후 저장 기간 동안 다양한 미생물과 질병에 의한 부패에 노출되기 쉽다. 수확 후 큐어링은 저장기간 동안 상처를 치유하고 미생물에 의한 부패를 방지하기 위한 가장 적합한 수단으로 알려져 있다. 본 연구에서는 큐어링과 연관된 분자적 기작에 관여하는 단백질들을 확인하기 위해, 큐어링 처리 후 저장기간 동안 단백질체의 변화를 분석하였다. 33℃ (큐어링) 및 15℃ (대조군)에서 3일 동안 처리하고 8주의 저장 기간이 지난 후 2D 전기영동 분석을 통해 단백질 spot의 변화를 확인한 결과, 31개 단백질 spot의 발현량이 차이 나는 것을 확인하였으며, 이들 중 15개의 단백질 spot을 동정하여 그 특성을 분석하였다. 동정된 단백질 중 alphaamylase (spot 1)는 큐어링 처리구에서만 발현량이 증가하였으며, probable aldo-keto reductase 2-like (spot 3) 및 hypothetical protein CHGG_01724 (spot 4)는 큐어링 및 대조구에서 동시에 발현량이 증가하였으나, sporamin A (spot 10)는 큐어링 및 대조구에서 발현량이 감소하였다. 한편, 대조구에서 enolase (spot 14)는 발현량이 증가하였으나, chain A of actinidin-E-64 complex+ (spot 19), ascorbate peroxidase (spot 22) 및 여러 sporamin 단백질들(spot 20, 21, 23, 24, 27, 29, 30 및 31)은 발현량이 감소하였다. 본 연구의 결과는 고구마 저장 뿌리에서 큐어링 처리와 관련된 단백질의 동정 및 수확 후 저장 기간동안 병 저항성과 관련된 기작에 대한 이해를 높이며, 향후 저온 저장 능력이 향상된 신품종 개발을 위한 후보 유전자의 도출에도 기여할 수 있을 것이다.

Keywords

Acknowledgement

본 연구는 한국연구재단 중견연구자지원사업(2021R1A2C400188711) 및 경상국립대학교 첨단소재분석지원센터의 지원으로 수행되었다.

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