Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Bacterial community comparison revealed by metagenomic analysis and physicochemical properties of eastern little tuna (Euthynnus affinis) with storage temperature differences

  • Asadatun Abdullah (Department of Aquatic Product Technology, Faculty of Fisheries and Marine Sciences, IPB University) ;
  • Rahadian Pratama (Molecular Science Research Group, Advanced Research Laboratory, IPB University) ;
  • Tati Nurhayati (Department of Aquatic Product Technology, Faculty of Fisheries and Marine Sciences, IPB University) ;
  • Windy Sibuea (Department of Aquatic Product Technology, Faculty of Fisheries and Marine Sciences, IPB University) ;
  • Sabila Diana Ahmad Sauqi (Department of Aquatic Product Technology, Faculty of Fisheries and Marine Sciences, IPB University)
  • Received : 2023.05.19
  • Accepted : 2023.08.16
  • Published : 2023.10.31
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Abstract

Post-harvest handling and hygienic level of aquatic products significantly affect the quality and level of safety. Cold chain control is one of the determining factors for the quality of fish and the bacterial community that grows on the fish. Identification of spoilage bacteria and pathogens in aquatic products must be made because it will determine the physical and chemical quality. A molecular identification method with high sensitivity is the solution. This study aims to identify the quality of fish and bacterial communities that grow. The research procedures included sample collection, pH measurement, drip loss measurement, transportation and cold storage treatment, DNA extraction, DNA sequencing, sequence analysis, and bioinformatics analysis. The conclusion obtained from this study is that the simulation of the cold chain system applied to eastern little tuna does not significantly affect changes in the water activity value, pH, and drip loss. The insignificant change indicates that the eastern little tuna samples are still in good quality. The bioinformatics analysis showed the highest diversity and abundance of the bacterial community came from the Gammaproteobacterial class.

Keywords

Acknowledgement

The authors would like to thank Molecular Laboratory of Advance Research Facility, IPB University for infrastructure. The opinions, findings and conclusions or recommendations expressed in this publication are that of the authors alone.

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