Journal of Applied Biological Chemistry

  • Volume 66
  • /
  • Pages.492-502
  • /
  • 2023
  • /
  • 1976-0442(pISSN)
  • /
  • 2234-7941(eISSN)
The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Anti-oxidant and immune enhancement effects of Artemisia argyi H. fermented with lactic acid bacteria

  • Ji Yun Lee (Department of Food Science and Nutrition, Pusan National University) ;
  • Ji Hyun Kim (Department of Food Science and Nutrition, Gyeongsang National University) ;
  • Ji Myung Choi (Department of Food and Nutrition, Kyungsung University) ;
  • Hyemee Kim (Department of Food Science and Nutrition, Pusan National University) ;
  • Weon Taek Seo (Department of Food Science and Technology, Gyeongsang National University) ;
  • Eun Ju Cho (Department of Food Science and Nutrition, Pusan National University) ;
  • Hyun Young Kim (Department of Food Science and Nutrition, Gyeongsang National University)
  • Received : 2023.11.22
  • Accepted : 2023.12.08
  • Published : 2023.12.31
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Abstract

This study investigated the antioxidant and immune enhancement activities of Artemisia argyi H. fermented by Lactobacillus plantarum. The fermented A. argyi H. ethanol extract increased scavenging activities of 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+), hydroxyl (·OH), and superoxide (O2-) radicals. Particularly, the ethanol extract of fermented A. argyi H. exhibited higher ·OH and O2- radical scavenging activities, compared with DPPH and ABTS+ radical scavenging activities. To evaluate the immune enhancement effects of the fermented A. argyi H., mice were fed a normal diet supplemented the fermented A. argyi H. at concentrations of 1%, 2%, and 5%, respectively. The supplementation of fermented A. argyi H. dose-dependently increased splenocyte proliferation. In addition, mice fed with 5% fermented A. argyi H. showed enhanced proliferation of T-cells and B-cells, along with increased levels of interferon-γ, interleukin-10, and tumor necrosis factor-α, compared to the normal group. Furthermore, mice fed with fermented A. argyi H. exhibited an increase in prominent probiotics such as Akkermansia muciniphila and Lactobacillus in gut microbiota, compared to the normal group. This study suggests that fermented A. argyi H. with Lactobacillus plantarum could be used as a dietary antioxidant and immune enhancement agent.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea(NRF) grant funded by the Korea government(MSIT) (No. 2021R1F1A1061542).

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