Journal of Applied Biological Chemistry

  • Volume 66
  • /
  • Pages.250-257
  • /
  • 2023
  • /
  • 1976-0442(pISSN)
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  • 2234-7941(eISSN)
The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Neuroprotective effects of Salacca wallichiana extract against glutamate-induced oxidative stress in mouse Hippocampal HT22 cells

쥐 해마 HT22 세포에서 글루타메이트 유도 산화 스트레스에 대한 Salacca wallichiana 추출물의 신경 보호 효과

  • Ji Hun Byeon (Faculty of Biotechnology, College of Applied Life Science, Jeju National University) ;
  • Ye Yeong Hong (Faculty of Biotechnology, College of Applied Life Science, Jeju National University) ;
  • Jungwhoi Lee (Subtropical/tropical Organism Gene Bank, Jeju National University) ;
  • Thet Thet Mar Win (Department of Botany, Yangon University) ;
  • Su Su Hlaing (Dawei University) ;
  • Song-I Han (Subtropical/tropical Organism Gene Bank, Jeju National University) ;
  • Jae Hoon Kim (Faculty of Biotechnology, College of Applied Life Science, Jeju National University)
  • Received : 2023.04.27
  • Accepted : 2023.06.02
  • Published : 2023.12.31
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Abstract

Glutamate is an excitatory neurotransmitter distributed in the central nervous system of mammals. However, high concentrations of glutamate are known to cause neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, and stroke by causing nerve cell death. In this study, the antioxidant activity and neuroprotective effect of subtropical natural products were analyzed. Among 11 subtropical plant extracts mainly tested, Sallacca wallichiana extract (SE) showed the greatest free radical scavenging activity. Then, we confirmed through WST-1 assay that SE protected HT22 cells against glutamate-induced cell death in a concentration-dependent manner. The protective effects of SE against glutamate-induced apoptosis in HT22 cells were also confirmed by flow cytometry analysis using Annexin V/PI double staining. We also confirmed using H2DCF-DA single staining that SE inhibits glutamate-induced intracellular reactive oxygen species. And we were confirmed through that SE inhibited glutamate-induced phosphorylation of Mitogen-activated Protein kinases. Consequently, our results propose that SE may contribute to the development of therapeutics to prevent neurodegenerative diseases.

Glutamate는 포유류의 중추신경계에 분포하는 흥분성 신경전달물질로, 기억, 인지, 그리고 학습 등에 있어서 중요한 역할을 한다. 하지만 고농도의 Glutamate는 신경세포에 독성을 유발하여 신경세포사멸을 유도함으로써 알츠하이머병, 파킨슨병, 뇌졸중 등의 신경퇴행성질환을 일으키는 것으로 알려져 있다. 본 연구에서 아열대 천연물의 항산화 활성과 신경보호 효과를 분석하였다. 11종의 아열대 추출물 중에서 Salacca wallichiana 추출물 (SE)의 라디칼 소거활성이 뛰어난 것으로 나타났다. 그리고 SE의 신경보호 효과를 조사한 결과 glutamate로 유도되는 cell death로부터 신경세포를 보호하였다. 또한 glutamate로 유도되는 apoptosis로부터 HT22 세포를 보호하는 효과는 Annexin V와 PI로 염색한 후 flow cytometry를 통해 분석되었다. 추가적으로 H2DCFDA 염색을 이용하여 SE가 glutamate로 유도되는 세포 내 활성 산소 종 (ROS)을 억제한다는 것을 확인했다. SE의 신경보호 효과는 oxidative stress로 유발되는 Mitogen-activated protein kinase (MAPK) signaling pathway를 억제함으로써 신경세포를 보호하는 것으로 나타났다. 결과적으로 SE가 신경퇴행성질환을 예방하기 위한 치료제 개발에 기여할 수 있음을 나타낸다.

Keywords

Acknowledgement

본 연구는 2016년도 정부(교육부)의 재원으로 한국연구재단의 지원을 받아 수행된 기초연구사업입니다(2016R1A6A1A03012862). HT22 세포를 배양해주신 이재란 연구원님께 감사의 말을 드립니다.

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