Journal of Applied Biological Chemistry

  • 제66권
  • /
  • Pages.1-6
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  • 2023
  • /
  • 1976-0442(pISSN)
  • /
  • 2234-7941(eISSN)
한국응용생명화학회 (The Korean Society for Applied Biological Chemistry)
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Bacterial neuraminidase inhibitory linarin from Dendranthema zawadskii

  • Ju Yeon Kim (Department of Pharmaceutical Engineering, IALS, Gyeongsang National University) ;
  • Jae Yeon Park (Department of Pharmaceutical Engineering, IALS, Gyeongsang National University) ;
  • Yun Gon Son (Department of Pharmaceutical Engineering, IALS, Gyeongsang National University) ;
  • Kyu Lim Kim (Department of Pharmaceutical Engineering, IALS, Gyeongsang National University) ;
  • Jeong Yoon Kim (Department of Pharmaceutical Engineering, IALS, Gyeongsang National University)
  • 투고 : 2022.12.14
  • 심사 : 2023.01.06
  • 발행 : 2023.12.31
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초록

Dendranthema zawadskii is a one of the popular plants as native in South Korea. In this study, linarin was isolated and purified using silica-gel, Diaion, and Sephadex LH-20 from the aerial parts of D. zawadskii. The chemical structure was completely identified through spectroscopic data including 1D, 2D nucleic magnetic resonance, and HRFABMS. Furthermore, linarin inhibited the bacterial neuraminidase (BNA) activity with 13.5 μM of IC50 dose-dependently. Through the enzyme kinetic experiments, linarin as BNA inhibitor exhibited a typical noncompetitive inhibition mode which Km was contestant and Vmax decreased as the concentration of the inhibitor increased. It was further identified that the inhibition constant was 16.0 μM. Linarin was the most abundance metabolite in the aerial part of D. zawadskii extract by UHPLC-TOF/MS analysis. Therefore, D. zawadskii and its main component are expected that it can be effectively used for the infection and inflammation caused by bacteria.

키워드

과제정보

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT), 2021R1A5A8029490 and 2022R1F1A1063786.

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