Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
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The effects of berberine on ischemia-reperfusion injuries in an experimental model of ovarian torsion

  • Filiz Yilmaz (Department of Histology and Embryology, Hitit University, Erol Olcok Research and Training Hospital, IVF Center) ;
  • Orkun Ilgen (Department of Obstetrics and Gynecology, Erzurum Research and Training Hospital) ;
  • Alper Mankan (Department of Obstetrics and Gynecology, Dokuz Eylul University, Faculty of Medicine) ;
  • Bayram Yilmaz (Pathology Department, Training and Research Hospital, Hitit University) ;
  • Sefa Kurt (Department of Obstetrics and Gynecology, Dokuz Eylul University, Faculty of Medicine)
  • Received : 2023.07.18
  • Accepted : 2023.08.31
  • Published : 2023.12.31
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Abstract

Objective: Ovarian torsion is a gynecological disorder that causes ischemia-reperfusion injuries in the ovary. Our study investigated berberine's short- and long-term effects on ovarian ischemia-reperfusion injuries. Methods: This study included 28 Wistar albino female rats weighing 180 to 220 g, which were divided into four groups: sham (S), torsion/detorsion (T/D), torsion/ detorsion+single dose berberine (T/D+Bb), and torsion/detorsion+15 days berberine (T/D+15Bb). The torsion and detorsion model was applied in all non-sham groups. In the T/D+Bb group, a single dose of berberine was administered, while in the T/D+15Bb group, berberine was administered over a period of 15 days. After the rats were euthanized, their ovaries were excised. The left ovaries were used for histopathologic evaluation, which included ovarian injury scoring and follicle count, while the right ovaries were used for biochemical analyses (tissue transforming growth factor-β [TGF-β] and alpha-smooth muscle actin [α-SMA] levels). Results: The histopathologic evaluation scores for the ovaries were significantly lower in the T/D+B group (p<0.05) and the T/D+15B group (p<0.005) than in the T/D group. The follicle counts in the T/D group were lower than those in both the sham and treated groups (p<0.005). The TGF-β levels were significantly lower in the T/D+15B group (p<0.005), whereas the α-SMA levels did not show a significant difference. Conclusion: Both short- and long-term berberine use could potentially have therapeutic effects on ovarian torsion. Long-term berberine use exhibited anti-inflammatory effects by reducing TGF-β levels, thereby preventing ischemia-reperfusion injuries. Therefore, we suggest that long-term berberine use could be beneficial for ovarian torsion.

Keywords

References

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