Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
권호 표지
DOI QR코드

DOI QR Code

Partial Lipectomy of the Epididymal Fat Alters Expression of the Steroidogenic Enzymes in the Mouse Testis at Different Postnatal Ages

  • Yong-Seung Lee (Department of Biological Science, Sungkyunkwan University) ;
  • Ki-Ho Lee (Department of Biochemistry and Molecular Biology, College of Medicine, Eulji University)
  • Received : 2023.09.15
  • Accepted : 2023.11.30
  • Published : 2023.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

The epididymal fat is a type of gonadal adipose tissue, which is localized closely to the testis. Even though it has been suggested that the epididymal fat is necessary for maintenance of spermatogenesis in the testis, the influence of epididymal fat on expression of testicular steroidogenic enzymes has not been examined. In the present research, expressional changes of steroidogenic enzymes in the mouse testis after 2 weeks of the surgical partial lipectomy of epididymal fat at different postnatal ages were determined by real-time polymerase chain reaction analysis. The transcript levels of all molecules at 2 months of postnatal age were significantly increased by the lipectomy of epididymal fat. However, the lipectomy at 5 months of postnatal age resulted in decreases of expression levels of all molecules examined in the testis. Except a reduced transcript level of hydroxysteroid 17-beta dehydrogenase 3, there were no significant changes of expression levels of other steroidogenic enzymes by the lipectomy at 8 months of postnatal age. At 12 months of postnatal age, the lipectomy caused a significant increase of transcript level of steroidogenic acute regulatory protein and a significant decrease of transcript level of hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1, without any expressional change of cytochrome P450 side chain cleavage, hydroxysteroid 17-beta dehydrogenase 3, and hydroxysteroid 17-beta dehydrogenase 3 in the testis. These findings suggest that the substances derived from epididymal fat could differentially influence on expression of steroidogenic enzymes in the testis during postnatal period.

Keywords

Acknowledgement

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2017R1D1A 1B03030209 and 2022R1F1A1076317).

References

  1. Altintas MM, Rossetti MA, Nayer B, Puig A, Zagallo P, Ortega LM, Johnson KB, McNamara G, Reiser J, Mendez AJ, Nayer A (2011) Apoptosis, mastocytosis, and diminished adipocytokine gene expression accompany reduced epididymal fat mass in long-standing diet-induced obese mice. Lipids Health Dis 10:198.
  2. Bjorntorp P, Karlsson M, Gustafsson L, Smith U, Sjostrom L, Cigolini M, Storck G, Pettersson P (1979) Quantitation of different cells in the epididymal fat pad of the rat. J Lipid Res 20:97-106. https://doi.org/10.1016/S0022-2275(20)40655-8
  3. Chu Y, Huddleston GG, Clancy AN, Harris RBS, Bartness TJ (2010) Epididymal fat is necessary for spermatogenesis, but not testosterone production or copulatory behavior. Endocrinology 151:5669-5679. https://doi.org/10.1210/en.2010-0772
  4. Cleary Margot P, Greenwood MRC, Brasel JA (1977) A multifactor analysis of growth in the rat epididymal fat pad. J Nutr 107:1969-1974. https://doi.org/10.1093/jn/107.11.1969
  5. Han J, Lee JE, Jin J, Lim JS, Oh N, Kim K, Chang SI, Shibuya M, Kim H, Koh GY (2011) The spatiotemporal development of adipose tissue. Development 138:5027-5037. https://doi.org/10.1242/dev.067686
  6. Kershaw EE, Flier JS (2004) Adipose tissue as an endocrine organ. J Clin Endocrinol Metab 89:2548-2556. https://doi.org/10.1210/jc.2004-0395
  7. Lee KH, Kim NH (2018) Expressional patterns of adipocyte-associated molecules in the rat epididymal fat during postnatal development period. Dev Reprod 22:351-360. https://doi.org/10.12717/DR.2018.22.4.351
  8. Lee KH, Kim NH (2019) Differential expression of adipocyte-related molecules in the distal epididymal fat of mouse during postnatal period. Dev Reprod 23:213-221. https://doi.org/10.12717/DR.2019.23.3.213
  9. Lee KH (2019) Postnatal expressional patterns of adipose-associated molecules in the mouse proximal epididymal fat. Dev Reprod 23:313-322. https://doi.org/10.12717/DR.2019.23.4.313
  10. Li L, Ma P, Huang C, Liu Y, Zhang Y, Gao C, Xiao T, Ren PG, Zabel BA, Zhang JV (2014) Expression of chemerin and its receptors in rat testes and its action on testosterone secretion. J Endocrinol 220:155-163.
  11. Nazian SJ (2013) Leptin secretion from the epididymal fat pad is increased by the sexual maturation of the male rat. J Androl 22:491-496. https://doi.org/10.1002/j.1939-4640.2001.tb02206.x
  12. Niemala S, Miettinen S, Sarkanen JR, Ashammakhi N (2008) Adipose tissue and adipocyte differentiation: Molecular and cellular aspects and tissue engineering applications. In: Ashammakhi N, Reis R, Chiellini F (eds), Topics in Tissue Engineering. Vol. 4. Biomaterials and Tissue Engineering Group, Porto, Portugal, pp 1-26.
  13. Nogueiras R, Luz Barreiro M, Caminos JE, Gaytán F, Suominen JS, Navarro VM, Casanueva FF, Aguilar E, Toppari J, Diéguez C, Tena-Sempere M. (2004) Novel expression of resistin in rat testis: Functional role and regulation by nutritional status and hormonal factors. J Cell Sci 117:3247-3257.
  14. Ocon-Grove OM, Krzysik-Walker SM, Maddineni SR, Hendricks III GL, Ramachandran R (2008) Adiponectin and its receptors are expressed in the chicken testis: Influence of sexual maturation on testicular ADIPOR1 and ADIPOR2 mRNA abundance. Reproduction 136:627-638. https://doi.org/10.1530/REP-07-0446
  15. Rak A, Mellouk N, Froment P, Dupont J (2017) Adiponectin and resistin: Potential metabolic signals affecting hypothalamo-pituitary gonadal axis in females and males of different species. Reproduction 153:R215-R226. https://doi.org/10.1530/REP-17-0002
  16. Tena-Sempere M, Barreiro ML (2002) Leptin in male reproduction: The testis paradigm. Mol Cell Endocrinol 188:9-13.
  17. Tirard J, Gout J, Lefrancois-Martinez AM, Martinez A, Begeot M, Naville D (2007) A novel inhibitory protein in adipose tissue, the aldo-keto reductase AKR1B7: Its role in adipogenesis. Endocrinology 148:1996-2005. https://doi.org/10.1210/en.2006-1707
  18. Ye H, Zhang HJ, Xu A, Hoo RLC (2013) Resistin production from adipose tissue is decreased in db/db obese mice, and is reversed by rosiglitazone. PLoS One 8:e65543.