The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Anti-Inflammatory Active Polysaccharide from Postbiotics of Cordyceps militaris Mycelium-Liquid Culture

동충하초(Cordyceps militaris) 균사체 액체발효 포스트바이오틱스로부터 항염증 활성다당 분리

  • Yeon Suk, Kim (Major in Food and Nutrition, Korea National University of Transportation) ;
  • Hyun Young, Shin (Transdisciplinary Major in Learning Health System, Dept. of Integrated Biomedical & Life Science, Korea University) ;
  • Hoon, Kim (College of Biotechnology & Natural Resources, Chung-Ang University) ;
  • Eun-Jin, Jeong (Transdisciplinary Major in Learning Health System, Dept. of Integrated Biomedical & Life Science, Korea University) ;
  • Hyun-Gyeong, Kim ( Dept. of Food & Nutrition, Sookmyung Women's University) ;
  • Min Geun, Suh (Transdisciplinary Major in Learning Health System, Dept. of Integrated Biomedical & Life Science, Korea University) ;
  • Hyung Joo, Suh (Transdisciplinary Major in Learning Health System, Dept. of Integrated Biomedical & Life Science, Korea University) ;
  • Kwang-Won, Yu (Major in Food & Nutrition, Korea National University of Transportation)
  • 김연숙 (한국교통대학교 식품영양학전공) ;
  • 신현영 (고려대학교 대학원 의생명융합과학과 러닝헬스시스템융합전공 ) ;
  • 김훈 (중앙대학교 생명공학대학 ) ;
  • 정은진 (고려대학교 대학원 의생명융합과학과 러닝헬스시스템융합전공 ) ;
  • 김현경 (숙명여자대학교 식품영양학과 ) ;
  • 서민근 (고려대학교 대학원 의생명융합과학과 러닝헬스시스템융합전공 ) ;
  • 서형주 (고려대학교 대학원 의생명융합과학과 러닝헬스시스템융합전공) ;
  • 유광원 (한국교통대학교 식품영양학전공)
  • Received : 2022.11.18
  • Accepted : 2023.01.06
  • Published : 2023.02.28
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Abstract

To investigate the anti-inflammatory activity of submerged culture using Cordyceps militaris mycelium, culture-including mycelia was extracted and lyophilized into postbiotics (hot-water extract; CM-HW). HW was fractionated into crude polysaccharide (CM-CP) by ethanol precipitation, and CM-CP was further dialyzed into CM-DCP by dialysis with running water using 12~14 kDa dialysis tube. When the cytotoxicity of subfractions against cells was assessed, no subfraction had a cytotoxic impact that was substantially different from the control groups. In an inflammatory model using LPS-stimulated RAW 264.7 cells, CM-DCP significantly decreased IL-6 and MCP-1 production levels compared to the LPS-control group. CM-DCP also inhibited IL-6 and IL-8 secretion in HaCaT keratinocytes stimulated with TNF-α and IFN-γ. In the meanwhile, the neutral sugar content and mannose ratio of anti-inflammatory CM-DCP were higher than the other fractions, and CM-DCP contained β-1,3/1,6-glucan of 216.1 mg/g. High pressure size exclusion chromatography revealed that CM-DCP contained molecules with a molecular weight range of 5.6 to 144.0 kDa. In conclusion, postbiotics of C. militaris mycelium significantly promoted anti-inflammatory activity, suggesting that neutral polysaccharides including Glc and Man contribute to the anti-inflammation in RAW 264.7 or HaCaT cells.

Keywords

Acknowledgement

본 논문은 2020년 전라북도 선도기업 육성사업(선도기업 기술개발 역량강화 지원사업; 과제번호 2020-나-6)으로 수행한 연구로 전북테크노파크와 (주)네오크레마에 감사드립니다.

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