Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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MS-5, a Naphthalene Derivative, Induces Apoptosis in Human Pancreatic Cancer BxPC-3 Cells by Modulating Reactive Oxygen Species

  • Suman, Giri (Department of Pharmacy, Daegu Catholic University) ;
  • Gyu Hwan, Park (College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University) ;
  • Joon-Seok, Choi (Department of Pharmacy, Daegu Catholic University) ;
  • Eunsook, Ma (Department of Pharmacy, Daegu Catholic University) ;
  • Kyung-Soo, Chun (College of Pharmacy, Keimyung University) ;
  • Sang Hoon, Joo (Department of Pharmacy, Daegu Catholic University)
  • Received : 2022.09.30
  • Accepted : 2022.10.17
  • Published : 2023.01.01
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Abstract

Pancreatic cancer is one of the most fatal cancers with a poor prognosis. Standard chemotherapies have proven largely ineffective because of their toxicity and the development of resistance. Therefore, there is an urgent need to develop novel therapies. In this study, we investigated the antitumor activity of MS-5, a naphthalene derivative, on BxPC-3, a human pancreatic cancer cell line. We observed that MS-5 was cytotoxic to BxPC-3 cells, as well as inhibited the growth of cells in a concentration- and time- dependent manner. Flow cytometry analysis revealed that the percentage of annexin V-positive cells increased after MS-5 treatment. We also observed cleavage of caspases and poly (ADP-ribose) polymerase, and downregulation of Bcl-xL protein. Flow cytometry analysis of intracellular levels of reactive oxygen species (ROS) and mitochondrial superoxide suggested that MS-5 induced the generation of mitochondrial superoxide while lowering the overall intracellular ROS levels. Thus, MS-5 may be potential candidate for pancreatic cancer treatment.

Keywords

Acknowledgement

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education (2017R1D1A1A02018536; 2021R1I1A3058531 to SJ and 2020R1l1A3066367 to KC).

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