Journal of Animal Reproduction and Biotechnology (한국동물생명공학회지)

The Korean Society of Animal Reproduction and Biotechnology (한국동물생명공학회)
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Expression of TASK-1 channel in mouse Leydig cells

  • Min Seok Woo (Department of Physiology, College of Medicine and Institute of Medical Sciences, Gyeongsang National University) ;
  • Eun-Jin Kim (Department of Physiology, College of Medicine and Institute of Medical Sciences, Gyeongsang National University) ;
  • Anjas Happy Prayoga (Department of Physiology, College of Medicine and Institute of Medical Sciences, Gyeongsang National University) ;
  • Yangmi Kim (Department of Physiology, Chungbuk National University College of Medicine) ;
  • Dawon Kang (Department of Physiology, College of Medicine and Institute of Medical Sciences, Gyeongsang National University)
  • Received : 2023.11.30
  • Accepted : 2023.12.12
  • Published : 2023.12.31
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Abstract

Background: Leydig cells, crucial for testosterone production, express ion channels like ANO1 that influence hormone secretion. This study investigates the expression and role of the Tandem of P domains in a weak inward rectifying K+ channel-related Acid-Sensitive K+-1 (TASK-1) channel in these cells, exploring its impact on testicular function and steroidogenesis. Methods: TASK-1 expression in Leydig cells was confirmed using immunostaining, while RT-PCR and Western Blot (WB) validated its expression in the TM3 Leydig cell line. The effect of a TASK-1 channel blocker on cell viability was assessed through live/dead staining and MTT assays. Additionally, the blocker's effect on testosterone secretion was evaluated by measuring testosterone levels. Results: Immunohistochemical analysis revealed a predominant presence of TASK-1, along with c-Kit and ANO-1, in Leydig cells adjacent to seminiferous tubules and also in Sertoli and spermatogenic cells. Expression levels of TASK-1 mRNA and protein were significantly higher in TM3 Leydig cells compared to TM4 Sertoli cells. In addition, blocking TASK-1 in TM3 cells with ML365 induced cell death but did not affect LH-induced testosterone secretion. Conclusions: These findings suggest that TASK-1 in Leydig cells is crucial for their viability and proliferation, highlighting its potential importance in testicular physiology.

Keywords

Acknowledgement

This work was supported by the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education (2021R1I1A3044128).

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