The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
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Molecular Detection of Phytoplasmas of the 16SrI and 16SrXXXII Groups in Elaeocarpus sylvestris Trees with Decline Disease in Jeju Island, South Korea

  • Geon-Woo, Lee (Department of Forest Environment Science, College of Agriculture and Life Science, Jeonbuk National University) ;
  • Sang-Sub, Han (Department of Forest Environment Science, College of Agriculture and Life Science, Jeonbuk National University)
  • 투고 : 2022.07.11
  • 심사 : 2023.01.11
  • 발행 : 2023.02.01
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초록

Phytoplasmas were discovered in diseased Elaeocarpus sylvestris trees growing on Jeju Island that showed symptoms of yellowing and darkening in the leaves. Leaf samples from 14 symptomatic plants in Jeju-si and Seogwipo-si were collected and phytoplasma 16S rRNA was successfully amplified by nested polymerase chain reaction using universal primers. The sequence analysis detected two phytoplasmas, which showed 99.5% identity to 'Candidatus Phytoplasma asteris' and 'Ca. P. malaysianum' affiliated to 16SrI and 16SrXXXII groups, respectively. Through polymerase chain reaction-restriction fragment length polymorphism (RFLP) analyses using the AfaI (RsaI) restriction enzyme, the presence of two phytoplasmas strains as well as cases of mixed infection of these strains was detected. In a virtual RFLP analysis with 17 restriction enzymes, the 16S rRNA sequence of the 'Ca. P. asteris' strain was found to match the pattern of the 16SrI-B subgroup. In addition, the phytoplasmas in the mixed-infection cases could be distinguished using specific primer sets. In conclusion, this study confirmed mixed infection of two phytoplasmas in one E. sylvestris plant, and also the presence of two phytoplasmas (of the 16SrI and 16SrXXXII groups) in Jeju Island (Republic of Korea).

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과제정보

This paper was supported by a project, "Investigation of Diagnosis and Characteristics of Yellow Disease on Elaeocarpus sylvestris trees (Project No. FE0100-2018-10-2022)" from the National Institute of Forest Science, Republic of Korea.

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