Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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The preventive effect of Mori Ramulus on oxidative stress-induced cellular damage in skeletal L6 myoblasts through Nrf2-mediated activation of HO-1

  • Cheol, Park (Division of Basic Sciences, College of Liberal Studies, Dong-eui University) ;
  • Hyesook, Lee (Anti-Aging Research Center, Dong-eui University) ;
  • Sung Ok, Kim (Department of Food and Nutrition, College of Life and Health, Kyungsung University) ;
  • Eun‑Woo, Lee (Biopharmaceutical Engineering Major, Division of Applied Bioengineering, College of Engineering, Dong-eui University) ;
  • Hyun‑Tai, Lee (Biopharmaceutical Engineering Major, Division of Applied Bioengineering, College of Engineering, Dong-eui University) ;
  • Hyun Ju, Kwon (Biopharmaceutical Engineering Major, Division of Applied Bioengineering, College of Engineering, Dong-eui University) ;
  • Byung Woo, Kim (Biopharmaceutical Engineering Major, Division of Applied Bioengineering, College of Engineering, Dong-eui University) ;
  • Gi‑Young, Kim (Department of Marine Life Science, Jeju National University) ;
  • Mi Ryeo, Kim (Department of Pharmacology, College of Korean Medicine, Daegu Haany University) ;
  • Yung Hyun, Choi (Anti-Aging Research Center, Dong-eui University)
  • Received : 2022.03.17
  • Accepted : 2022.06.20
  • Published : 2023.01.15
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Abstract

The aim of the present study is to investigate the preventive effect of water extract of Mori Ramulus (MRWE) on oxidative stress-mediated cellular damages in rat skeletal L6 myoblasts. Our results demonstrated that MRWE pretreatment markedly improved cell survival and suppressed cell cycle arrest at the G2/M phase and apoptosis in hydrogen peroxide (H2O2)-treated L6 cells. H2O2-triggered DNA damage was also notably reduced by MRWE, which since it was correlated with protection of reactive oxygen species (ROS) production. Additionally, H2O2 stimulated cytosolic release of cytochrome c and up-regulation of Bax/Bcl-2 ratio, whereas MRWE suppressed these changes following by H2O2. Moreover, MRWE inhibited the cleavage of poly(ADP-ribose) polymerase as well as the activity of caspase-3 by H2O2. Furthermore, MRWE enhanced H2O2-mediated expression of nuclear factor erythroid 2-associated factor 2 (Nrf2) and its representative downstream enzyme, heme oxygenase-1 (HO-1). However, the protective effects of MRWE on H2O2-induced ROS production, cell cycle arrest and apoptosis were significantly attenuated by HO-1 inhibitor. In conclusion, our present results suggests that MRWE could protect L6 myoblasts from H2O2-induced cellular injury by inhibiting ROS generation along with Nrf2-mediated activation of HO-1, indicating this finding may expand the scope of application of Mori Ramulus in medicine.

Keywords

Acknowledgement

This research was funded by the National Research Foundation of Korea Grant (NRF-2021R1A2C201471711 and 2021R1A2C200954911) and Korea Basic Science Institute Grant funded (2020R1A6C101A201)

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