한국식품저장유통학회지 (Food Science and Preservation)

  • 제30권4호
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  • Pages.716-728
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  • 2023
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  • 3022-5477(pISSN)
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  • 3022-5485(eISSN)
한국식품저장유통학회 (The Korean Society of Food Preservation)
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감초 주정추출물의 3T3-L1 지방세포 분화 억제 및 고지방 식이로 유도된 C57BL/6J 마우스에 대한 항비만 효과

Anti-obesity effects of Glycyrrhiza uralensis ethanol extract on the inhibition of 3T3-L1 adipocyte differentiation in high-fat diet-induced C57BL/6J mice

  • 박선경 (한국식품연구원 맞춤형식이연구단) ;
  • 이장호 (한국식품연구원 맞춤형식이연구단) ;
  • 박수현 (한국식품연구원 맞춤형식이연구단) ;
  • 이유건 (한국식품연구원 맞춤형식이연구단)
  • Seon Kyeong Park (Personalized Diet Research Group, Korea Food Research Institute (KFRI)) ;
  • Jangho Lee (Personalized Diet Research Group, Korea Food Research Institute (KFRI)) ;
  • Soo Hyun Park (Personalized Diet Research Group, Korea Food Research Institute (KFRI)) ;
  • Yu Geon Lee (Personalized Diet Research Group, Korea Food Research Institute (KFRI))
  • 투고 : 2023.07.03
  • 심사 : 2023.08.07
  • 발행 : 2023.08.30
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초록

감초의 항비만 소재로서 가능성을 확인하기 위해 고지방 식이로 유도된 마우스 모델과 3T3-L1 지방 전구세포의 지방세포 분화과정에 GUE의 효과를 평가하였다. GUE의 섭취는 고지방 식이로 인해 증가한 마우스 체중을 유의하게 감소시켰다. 또한, GUE 처리로 인해 3T3-L1 지방 전구세포의 지방세포 분화과정에 의해 증가한 지방구 형성이 효과적으로 감소하는 것을 확인하였다. 이런 억제 효과는 GUE가 지방세포 분화과정에서 증가한 PPARγ 및 C/EBPα의 발현을 효과적으로 억제하기 때문이며, 이는 지방 대사 조절 인자들의 발현억제에도 영향을 주었다. 또한, LC-Q-TOF-MS 분석을 통해 GUE 주요 생리활성 물질을 분석한 결과, liquiritigenin 배당체 및 licorice saponins를 포함한 12종의 주요 생리활성 물질들이 동정되었다. 이러한 결과들을 종합하여 볼 때, GUE의 항비만 효과는 우수한 생리활성을 가지는 phytochemical들에 의한 지방 대사 조절 인자들의 발현조절과 관련이 깊다고 판단되며, 감초의 비만을 포함한 대사성질환 예방 소재로서 활용 가치를 높이는데 유용할 것으로 생각된다.

The anti-adipogenic activity of Glycyrrhiza uralensis was investigated by examining the effects of its ethanol extract (GUE) on a mouse model with a high-fat diet (HFD) and 3T3-L1 preadipocytes during adipocyte differentiation. GUE administration for eight weeks significantly reduced weight gain in mice fed an HFD. GUE effectively inhibited 3T3-L1 preadipocyte differentiation and lipid droplet accumulation. This inhibitory effect is associated with the downregulation of key adipogenic regulators, including PPARγ and C/EBPα, and the modulation of adipose metabolism regulators, such as Fasn and Fabp4. LC-Q-TOF-MS analysis identified twelve phenolic and flavonoid compounds, including liquiritigenin and licorice saponin, in the GUE. These findings demonstrate that the anti-obesity effect of the GUE is attributed to the biological activity of its phenolic and flavonoid compounds. Therefore, the GUE has potential anti-obesity activity. Moreover, further studies on the isolation of bioactive components from the GUE and the investigation of the underlying molecular mechanisms of the GUE are required to establish its efficacy in metabolic disorders, including obesity.

키워드

과제정보

본 연구는 한국식품연구원 주요연구개발사업(E0210601)의 지원을 받아 수행한 연구 결과로 이에 감사드립니다.

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