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Anti-fatigue effect of a beverage mixture containing red ginseng and Prunus mume fruit vinegar on high-intensity exercised rats

홍삼과 매실식초 혼합 음료의 고강도 운동을 실시한 흰쥐에서 항피로 효과

  • Wool-Lim Park (Department of Food Biotechnology, Dong-A University) ;
  • Jeong-Ho Kim (Suncheon Research Center for Bio Health Care) ;
  • Kwon-Il Seo (Department of Food Biotechnology, Dong-A University)
  • 박울림 (동아대학교 식품생명공학과) ;
  • 김정호 (순천바이오헬스케어연구센터) ;
  • 서권일 (동아대학교 식품생명공학과)
  • Received : 2023.03.27
  • Accepted : 2023.05.12
  • Published : 2023.06.30

Abstract

New types of vinegar drinks are being processed and developed using various raw materials. In this study, a beverage containing a mixture of 0.25% red ginseng extract and 5% Prunus mume fruit vinegar (RPV) was evaluated for its anti-fatigue effect on high-intensity exercised rats. RPV administration markedly enhanced running endurance and significantly decreased fatigue-related serum biomarkers, such as inorganic phosphate, ammonia, and L-lactate, compared to the other groups. In addition, RPV administration increased glycogen contents in the liver and muscles and decreased creatine kinase activity in the serum and muscles. RPV administration also remarkedly increased the activity of lactate in the muscles. Furthermore, HPLC analysis revealed that main organic acids in RPV were acetic acid, malic acid, and citric acids. Overall, the results indicate that RPV improved fatigue recovery in exhausted rats, thus proving a promising material of functional food to attenuate fatigue.

본 연구는 매실식초 및 홍삼농축액 혼합 음료의 증진된 피로회복 효과를 확인하고자 유기산 함량과 고강도 운동 쥐모델에서 항피로 관련 인자를 측정하였다. 홍삼농축액 및 매실식초 혼합 음료 내 유기산 함량은 acetic acid가 1,654.17 ppm으로 가장 높은 함량을 보였고, malic acid와 citric acid가 각각 617.28, 600.12 ppm으로 확인되었다. 항피로 관련 인자 중 지구력을 측정한 결과, 홍삼매실식초 혼합음료(RPV)를 섭취한 군에서 138.83 min으로 가장 높은 지구력을 보였으며, 혈중 무기인산과 암모니아 및 젖산 함량은 0.67 mM, 24.28 mmol/L 및 1.75 mM으로 유의적으로 가장 낮은 수치를 보였다. 또한, 간 및 근육 내 글리코겐 함량에서 RPV는 각각 288.4, 22.84 ㎍/g으로 가장 높은 함량을 나타냈다. RPV의 혈청 및 근육조직 내 creatine kinase 함량은 465.42와 33.05 U/L로 유의적으로 가장 낮은 값으로 확인되었으며, 근육 내 lactate dehydrogenase 함량은 RPV에서 가장 높은 값인 140.13 U/L로 나타났다. 본 연구결과를 통해 풍부한 유기산을 가진 매실식초 및 홍삼농축액 혼합 음료의 섭취는 매실식초 또는 홍삼농축액의 단일 섭취에 비해 증진된 항피로 효과를 나타낸다는 것을 확인하였으며, 이는 피로회복 기능성식품 소재로 이용할 수 있을 것으로 기대한다.

Keywords

Acknowledgement

This work was supported by the grant from The Korean Society of Ginseng (2020).

References

  1. Bae DK, Choi HJ, Son JH, Park MH, Bae JH, An BJ, Bae MJ, Choi C. The study of developing and stability of function beverage from Korean persimmon (Diospyros kaki L. folium ) leaf. Korean J Food Sci Technol, 32, 860-866 (2000)
  2. Bal JS, Jawanoda JS, Singh SN. Development physiology of ber (Zizyphus mauritina ) var. urman. IV. Change in amino acids and sugar at different stages of fruit ripening. India FD Pckr, 33, 3335-3337 (1979)
  3. Banfi G, Lombardi G, Colombini A, Melegati G. Whole-body cryotherapy in athletes. Sports Med, 40, 509-517 (2010)
  4. Cho HD, Kim JH, Lee JH, Hong SM, Yee ST, Seo KI. Anti-fatigue effect of a cucumber vinegar beverage on rats after high-intensity exercise. Korean J Food Sci Technol, 49, 209-214 (2017) https://doi.org/10.9721/KJFST.2017.49.2.209
  5. Cho HD, Lee JH, Jeong JH, Kim JY, Yee ST, Park SK, Lee MK, Seo KI. Production of novel vinegar having antioxidant and anti-fatigue activities from Salicornia herbacea L. J Sci Food Agric, 96, 1085-1092 (2016) https://doi.org/10.1002/jsfa.7180
  6. Chu GX, Chen X. Anti-lipid peroxidation and protection of ginsenosides against cerebral ischemia-reperfusion injuries in rats. Acta Pharmacol Sin, 11, 119-123 (1990)
  7. Furukawa S, Udea R. Studies on non-volatile organic acid in vinegar, contents of non-volatile organic acid in commercial vinegars. J Fement Technol, 41, 14-19 (1963)
  8. Fushimi T, Sato Y. Effect of acetic acid feeding on the circadian changes in glycogen and metabolites of glucose and lipid in liver and skeletal muscle of rats. Br J Nutr, 94, 714-719 (2005) https://doi.org/10.1079/BJN20051545
  9. Fushimi T, Suruga K, Oshima Y, Fukiharu M, Tsukamoto Y, Goda T. Dietary acetic acid reduces serum cholesterol and triacylglycerols in rats fed a cholesterol-rich diet. Br J Nut, 95, 916-924 (2006) https://doi.org/10.1079/BJN20061740
  10. Fushimi T, Tayama K, Fukaya M, Kitakoshi K, Nakai N, Tsukamoto Y, Sato Y. Acetic acid feeding enhances glycogen repletion in liver and skeletal muscle of rats. J Nutr, 131, 1973-1977 (2001) https://doi.org/10.1093/jn/131.7.1973
  11. Guan T, Li S, Guan Q, Shi JS, Lu ZM, Xu ZH, Geng Y. Spore powder of Paecilomyces hepiali shapes gut microbiota to relieve exercise-induced fatigue in mice. Nutrients, 14, 2973 (2022)
  12. Ha TY, Lee JH, Kim SH. Effect of Panax ginseng on the graft-versus-host reaction, production of leucocyte migration inhibitory factor and expulsion of adult Trichinella spiralis in mice. J Korea Soc Microbiol, 21, 133-144 (1986)
  13. Hsiao CY, Hsu YJ, Tung YT, Lee MC, Huang CC, Hsieh CC. Effects of Antrodia camphorata and Panax ginseng supplementation on anti-fatigue properties in mice. J Vet Med Sci, 80, 284-291 (2018) https://doi.org/10.1292/jvms.17-0572
  14. Hwang HJ, Kim JS, Jang JW, Lim KW, Joung SS, Choi SK. The effect of thiamine and endurance training of 4 weeks for PDH activity in skeletal muscle. Korean J Phys Educ, 55, 649-658 (2016)
  15. Jeong YJ, Lee MH, Seo KI, Kim JN, Lee YS. The quality comparison of grape vinegar by two stages fermentation with traditional grape vinegar. J East Asian of Dietary Life, 8, 462-468 (1998)
  16. Jeong YJ, Seo JH, Jung SH, Shin SR, Kim KS. The quality comparison of uncleaned rice vinegar by two stages fermentation with commercial uncleaned rice vinegar. Korean J Postharvest Sci Technol, 5, 374-379 (1998)
  17. Jeong YJ, Seo KI, Kim KS. Physicochemical properties of marketing and intensive persimmon vinegars. J East Asian Soc Diet Life, 6, 355-363 (1996)
  18. Jo GS, Chai HY, Ji HJ, Kang MH, Kang SJ, Ji JG, Kim DJ, Lee BJ. Enhancement of exercise capaticy by black ginseng extract in rats. Lab Anim Res, 26, 279-286 (2010) https://doi.org/10.5625/lar.2010.26.3.279
  19. Johnston CS, White AM, Kent SM. A preliminary evaluation of the safety and tolerance of medicinally ingested vinegar in individuals with type 2 diabetes. J Med Food, 11, 179-183 (2008) https://doi.org/10.1089/jmf.2007.574
  20. Juel C, Klarskov C, Nielsen JJ, Krustrup P, Mohr M, Bangsbo J. Effect of high-intensity intermittent training on lactate and H+ release from human skeletal muscle. Am J Physiol Endocrinol Metab, 286, 245-251 (2004) https://doi.org/10.1152/ajpendo.00303.2003
  21. Kang MJ, Hwang CR, Lee SJ, Shin JH. Antifatigue effect of eel and plant mix extracts during aerobic running training in Sprague Dawley rats. J Life Sci, 24, 728-736 (2014) https://doi.org/10.5352/JLS.2014.24.7.728
  22. Kim CH, Kim MB, Lee SH, Kim YJ, Hwang JK. Ashitaba and red ginseng complex stimulates exercise capacity by increasing mitochondrial biogenesis. Korean J Food Sci Technol, 49, 685-692 (2017)
  23. Kim DH, Kim KT, Lee KK. The effect of drinking the plum extract on the college soccer player' cardiovascular system and blood lactated. Korea J Sport Sci, 24, 1381-1391 (2015)
  24. Kim JH, Cho HD, Won YS, Hong SM, Moon KD, Seo KI. Anti-fatigue effect of Prunus mume vinegar in high-intensity exercised rats. Nutrients, 12, 1205 (2020)
  25. Kim KJ, Bae YS, Lee SC, Lee WJ, Lee IK, Yoon YK, Yoo JS, Park HK, Ha WH. Influence of vinegar-drink with persimmon on oxygen transport function and recovery capacity in exercise. Korean J Phys Education, 36, 102-113 (1997)
  26. Kim MJ, Choi JH, Kwon SH, Kim HD, Bang MH, Yang SA. Characteristics of fermented dropwort extract and vinegar using fermented dropwort extract and its protective effects on oxidative damage in rat glioma C6 cells. Korean J Food Sci Technol, 45, 350-355 (2013) https://doi.org/10.9721/KJFST.2013.45.3.350
  27. Kim SN, Ha YW, Shin H, Son SH, Wu SJ, Kim YS. Simultaneous quantification of 14 ginsenosieds in Panax ginseng C.A. Meyer (Korean red ginseng) by HPLC-ELSD and its application to quality control. J Pharm Biomed Anal, 45, 164-170 (2007) https://doi.org/10.1016/j.jpba.2007.05.001
  28. Ko YJ, Jeong DY, Lee JO, Park MH, Kim EJ, Kim JW, Kim YS, Ryu CH. The establishment of optimum fermentation conditions for Prunus mume vinegar and its quality evaluation. J Korean Soc Food Sci Nutr, 36, 361-365 (2007) https://doi.org/10.3746/jkfn.2007.36.3.361
  29. Kumar CT, Reddy VK, Thyagarajuk Prasady M, Reddanna PP. Dietary supplementation of vitamin E products heart tissue exercise induced oxidant stress. Mol CELL Biochem, 111, 109-115 (1992) https://doi.org/10.1007/BF00229581
  30. Lee DS, Ryu IH, Lee KS, Shin YS, Chun SH. Optimization in the preparation of Aloe vinegar by Acetobacter sp. And inhibitory effect against lipase activity. J Korean Soc Agric Chem Biotechnol, 42, 105-110 (1999)
  31. Lee JW, Ko HR, Shim KH. Structural characteristics of the water soluble browning reaction products isolated from Korean red ginseng. Korean J Food Nutr, 11, 499-505 (1998)
  32. Lim EJ, Cha GH. Study on manufacturing of vinegar through literatures of the Joseon dynasty. Korean J Food Culture, 25, 680-707 (2010)
  33. Ortenblad N, Westerblad H, Nielsen J. Muscle glycogen stores and fatigue. J Physiol, 591, 4405-4413 (2013) https://doi.org/10.1113/jphysiol.2013.251629
  34. Paik IY, Chang WR, Kwak YS, Cho SY, Jin HE. The effect of Prunus mume supplementation on energy substrate levels and fatigue induction factors. J Life Sci, 20, 49-54 (2010) https://doi.org/10.5352/JLS.2010.20.1.049
  35. Park SG. Effect of Maesil extracts on the blood components of women bad minton players. J Korean Sports Med, 8, 40-43 (1990)
  36. Park SK, Kyung KH. Stimulatory effect of oxygen on the browning of a soy sauce meodel system. Korean J Food Sci Technol, 23, 523-525 (1991)
  37. Park SY. The effect of sport drink on heart rate and lactate after exercise. Korean J Phys Educ, 34, 182-191 (1995)
  38. Robergs RA, Ghiasvand F, Parker D. Biochemistry of exercised-induced metabolic acidosis. Am J Physiol Regul Integr Comp Physiol, 287, 502-516 (2004) https://doi.org/10.1152/ajpregu.00114.2004
  39. Ryu S, Kwon TD. Effects of brown-rice vinegar ingestion on energy metabolism during moderate intensity exercise. Korean J Exer Nutr, 13, 217-224 (2009)
  40. Sheo HJ, Lee MY, Chung DL. Effect of Prunus mume extract on gastric secretion in rats and carbon tetrachloride induced liver damage of rabbits. J Korean Soc Food Sci Nutr, 19, 21-26 (1990)
  41. Shin JH, Choi DJ, Lee SJ, Cha JY, Sung NJ. Antioxidant activity of black garlic (Allium sativum L.). J Korean Soc Food Sci Nutr, 37, 965-971 (2008) https://doi.org/10.3746/jkfn.2008.37.8.965
  42. Sung NH, Jeong YJ. Consumer awareness survey on functional properties of vinegar beverage. Food Ind Nutr, 18, 37-42 (2013)
  43. Uemura T, Yamamoto A, Miura R, Yamano T. Microsomal methoxymelanin formation from 6-hydroxymelatonin. FEBS Lett, 122, 237-240 (1980) https://doi.org/10.1016/0014-5793(80)80447-9
  44. Xu C, Lv J, Lo YM, Cui SW, Hu X, Fan M. Effects of oat β-glucan on endurance exercise and its anti-fatigue properties in trained rats. Carbohydr Polym, 92, 1159-1165 (2013)
  45. Yang JO, Yoo CJ, Kim JO, Che ME. Utilization fermented tea-fungus beverage for the sports drink. Kor J Phys Edu, 38, 277-293 (1999)
  46. Yang Y, Wang H, Zhang M, Shi M, Yang C, Ni Q, Wang Q, Li J, Wang X, Zhang C, Li Z. Safety and antifatigue effect of Korean red ginseng capsule: A randomized, double-blind and placebo-controlled clinical trial. J Ginseng Red, 46, 543-549 (2022) https://doi.org/10.1016/j.jgr.2021.09.001
  47. Yokozawa T, Suzuki N, Zheng PD, Oura H, Nishioka I. Effect of orally administered rhubarb extract in rats with chronic renal failure. Chem Pharm Bull, 32, 4506-4513 (1984) https://doi.org/10.1248/cpb.32.4506
  48. Yoon SJ, Park HC, Lee JS, Choi YJ, Ahn SK, Kim NM, Choi YG. Effects of red ginseng and mixed red ginseng supplementation on aerobic.anaerobic performance and fatigue recovery rate. J Sport Leis Stud, 47, 855-866 (2012)
  49. Zheng Y, Zhang WC, Wu ZY, Fu CX, Hui AL, Gao H, Chen PP, Du B, Zhang HW. Two macamide extracts relieve physical fatigue by attenuating muscle damage in mice. J Sci Food Agric, 99, 1405-1412 (2018) https://doi.org/10.1002/jsfa.9318