Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Evaluation of radical scavenging and diasestive enzyme inhibitory capacities of peach twigs fraction extract (Prunus persica L. Bastch)

복숭아 나뭇가지 분획추출물의 라디칼 소거 및 소화효소 저해활성 평가

  • Youjeoung Lee (Department of Food Science and Biotechnology, Daegu University) ;
  • Gyeong Han Jeong (Research Division for Biotechnology, Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI)) ;
  • Ju Yeon Hong (Department of Food Nutrition, Daegu Haany University) ;
  • Tae Hoon Kim (Department of Food Science and Biotechnology, Daegu University)
  • 이유정 (대구대학교 식품공학과) ;
  • 정경한 (한국원자력연구원 첨단방사선연구소 방사선바이오연구부) ;
  • 홍주연 (대구한의대학교 식품영양학과) ;
  • 김태훈 (대구대학교 식품공학과)
  • Received : 2022.11.30
  • Accepted : 2022.12.23
  • Published : 2023.02.28
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Abstract

We investigated the free radical scavenging and digestive enzyme inhibitory activities of the hot water extract of peach twig (Prunus persica L. Bastch). This extract of the peach twigs was further split up into n-hexane, ethyl acetate (EtOAc), and n-butyl alcohol(n-BuOH), which resulted in three solvent-soluble fractions. Free radical scavenging activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+) assay systems, while hypoglycemic effect of the peach twig extract and the solvent-soluble fractions were tested using α-glucosidase and α-amylase inhibition assays. Accordingly, the EtOAc layer showed a greater free radical scavenging activity compared to other solvent-soluble fractions. Furthermore, based on the α-glucosidase and α-amylase assays, the IC50 values were determined to be 38.2±1.6 and 69.6±6.1 ㎍/mL for the EtOAc-soluble fractions, respectively. Taken together, these results suggest that the fractions obtained from the peach twig extract can be considered as a potential source of natural antioxidant and hypoglycaemic constituents.

본 연구에서는 복숭아 나뭇가지를 이용하여 열수 추출하고 얻어진 추출물에 대해 유기용매를 이용하여 극성별 분획을 실시하였으며, 열수추출물 및 분획물의 항산화 활성과 관련된 총페놀 함량, DPPH 및 ABTS+ 라디칼 소거능, α-glucosidase 및 α-amylase 저해능을 측정하였다. DPPH 라디칼 소거능 측정에서는 페놀성 물질의 함량을 가장 많이 나타낸 EtOAc 가용부의 IC50값이 3.82±1.6 ㎍/mL로 가장 우수한 활성을 나타내었고, ABTS+ 라디칼 소거활성 평가에서도 EtOAc 분획물에서 IC50값이 16.5±0.7 ㎍/mL로 강한 라디칼 소거활성을 나타내었다. 항당뇨 활성과 관련된 효소인 α-glucosidase 및 α-amylase 저해능 평가에서도 우수한 라디칼소거능을 나타낸 EtOAc 분획물에서 IC50값이 각 38.2±1.6 및 69.6±6.1 ㎍/mL로 다른 유기용매 분획물에 비해 우수한 저해 활성임을 확인하였다. 이러한 결과는 복숭아 나뭇가지 추출물이 항산화 및 항당뇨 효능을 가지며, 새로운 천연물 유래의 기능성 신소재 발굴을 위한 기초자료로 이용 가능할 것으로 사료된다.

Keywords

Acknowledgement

This research was supported (in part) by the Daegu University, 2021.

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