Journal of Society of Preventive Korean Medicine (대한예방한의학회지)

Society of Preventive Korean Medicine (대한예방한의학회)
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Relieving effect for respiratory inflammation of Gumiganghwal-tang

구미강활탕(九味羌活湯)의 호흡기 염증 완화효과

  • Bo-In Kwon (Research Institute of Korean Medicine, Sangji University) ;
  • Joo-Hee Kim (Research Institute of Korean Medicine, Sangji University)
  • 권보인 (상지대학교 한의학연구소) ;
  • 김주희 (상지대학교 한의학연구소)
  • Received : 2023.12.03
  • Accepted : 2023.12.17
  • Published : 2023.12.31
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Abstract

Objectives : Gumiganghwal-tang and its main components have been used for treatment of cough, headache, joint pain and fever. Using a respiratory inflammatory model, we intend to demonstrate the its anti-inflammatory effect and immune mechanism of Gumiganghwal-tang. Methods : We induced the respiratory inflammation mouse model by papain treatment. Female BALB/C mice (8 weeks old) were divided into three groups as follows: saline control group, papain treatment group (vehicle), papain and Gumiganghwal-tang (200 mg/kg) treatment group (n=4). To verify the anti-inflammatory effect of Gumiganghwal-tang extracts, we measured the infiltration of inflammatory cells in bronchoalveolar lavage fluid (BALF) and nasal lavage fluid (NALF). Additionally, the efficacy of Gumiganghwal-tang extracts on Th2 cell population and alveolar macrophage in lung were analyzed by using flow cytometry. Results : Gumiganghwal-tang extracts administration decreased inflammatory cell infiltration in BALF and NALF, especially of eosinophils. Furthermore, interleukin-5 level was reduced in lung by drug administration. Interestingly, Gumiganghwal-tang extracts treatment also decreased the Th2 cell (CD4+GATA3+) population and increased the alveolar macrophage (CD11b+CD11c+) population in lung. Conclusions : Our findings indicate that Gumiganghwal-tang extracts have anti-inflammatory effects by mediating Th2 cell and alveolar macrophage cell activation.

Keywords

Acknowledgement

본 결과물은 2023년도 교육부의 재원으로 한국연구재단의 지원을 받아 수행된 지자체-대학 협력 기반 지역혁신 사업의 결과입니다(2022RIS-005).

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