Journal of Korean Medicine for Obesity Research (한방비만학회지)

The Society of Korean Medicine for obesity Research (한방비만학회)
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A Comparative Study on the Efficacy and Mechanism of Improving Glucose Uptake of Cannabis Root and Stem Extracts

대마 뿌리 및 줄기 추출물의 포도당 흡수 개선 효과 및 기전에 대한 비교 연구

  • Hye-Lin Jin (Department of Diagnostics, College of Korean Medicine, Dongguk University) ;
  • Ga-Ram Yu (Department of Diagnostics, College of Korean Medicine, Dongguk University) ;
  • Hyuck Kim (Department of Diagnostics, College of Korean Medicine, Dongguk University) ;
  • Kiu-Hyung Cho (Gyeongbuk Institute for Bio Industry) ;
  • Ki-Hyun Kim (Gyeongbuk Institute for Bio Industry) ;
  • Dong-Woo Lim (Department of Diagnostics, College of Korean Medicine, Dongguk University)
  • 진혜린 (동국대학교 한의과대학 진단학교실) ;
  • 유가람 (동국대학교 한의과대학 진단학교실) ;
  • 김혁 (동국대학교 한의과대학 진단학교실) ;
  • 조규형 (경북바이오산업연구원 대마산업총괄지원단) ;
  • 김기현 (경북바이오산업연구원 대마산업총괄지원단) ;
  • 임동우 (동국대학교 한의과대학 진단학교실)
  • Received : 2023.07.22
  • Accepted : 2023.08.29
  • Published : 2023.12.31
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Abstract

Objectives: Despite the pharmacological potential of the roots and stems of hemp based on literatures, active research has not been conducted for a long time. Comparative experiments were conducted on antioxidant and anti-inflammatory effects and improvement of glucose uptake using Cannabis root and stem extracts. Methods: Antioxidant contents in Cannabis root and stem extracts were examined with total phenolic, tannin, flavonoid assay. Anti-inflammatory properties were tested in lipopolysaccharides-treated RAW264.7 cells. Efficacy of Cannabis root and stem extracts on glucose uptake was investigated using fluorescent glucose analog (2-NBDG) in palmitate-treated HepG2 cells. The mechanism of action on metabolism was examined by western blot. Results: Antioxidant and anti-inflammatory efficacy were greater in stem extracts, but improvements in glucose uptake performed under various conditions were found to be greater in root extracts. It is assumed that Cannabis root extracts exhibited an improvement in glucose uptake through mechanisms such as AMP-activated protein kinase activation, not depending on general antioxidant and anti-inflammatory effects. Conclusions: Further research is needed on the mechanisms and substances that exhibit the anti-diabetic effects of Cannabis roots and stems.

Keywords

Acknowledgement

본 연구는 한국보건산업진흥원을 통해 보건복지부 '한의약 혁신기술개발사업'의 재정 지원을 받아 수행된 연구 임(과제고유번호: HF23C0112).

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