Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Detection of Xanthomonas hortorum pv. carotae in Jeju Island Soils after Carrot Harvest

수확 후 제주 당근 재배 토양에서 Xanthomonas hortorum pv. carotae 분리

  • Mi-Jin Kim (College of Applied Life Sciences, Major of Plant Resources & Environment, Jeju National University) ;
  • Hyun Su Kang (College of Applied Life Sciences, Major of Plant Resources & Environment, Jeju National University) ;
  • Yong Ho Shin (College of Applied Life Sciences, Major of Plant Resources & Environment, Jeju National University) ;
  • Yong Chull Jeun (College of Applied Life Sciences, Major of Plant Resources & Environment, Jeju National University)
  • 김미진 (제주대학교 생명자원과학대학 친환경연구소) ;
  • 강현수 (제주대학교 생명자원과학대학 친환경연구소) ;
  • 신용호 (제주대학교 생명자원과학대학 친환경연구소) ;
  • 전용철 (제주대학교 생명자원과학대학 친환경연구소)
  • Received : 2023.09.13
  • Accepted : 2023.11.08
  • Published : 2023.12.31
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Abstract

Bacterial leaf blight in carrot is one of the most important diseases in the worldwide. In the past decade, its introduction into Korea is causing great concern due to the potential damage to carrot crops domestically. This bacterial disease is caused by Xanthomonas hortorum pv. carotae (Xhc). This study aimed to isolate and identify bacterial strains from the soil of carrot farms in Jeju Island. The bacterial isolates showing characteristics similar with those of Xhc were selected when cultured on artificial media. Through DNA sequencing and analysis based on NCBI data, some of the selected bacterial strains were identified as Xhc. Furthermore, the bacterial strains caused the typical symptom of bacterial leaf blight after inoculation on carrot leaves. The results of this study showed the potential establishment of Xhc in the soil of Jeju Island and it may be valuable data for establish a strategy preventing the domestic spread of carrot bacterial leaf blight in the future.

당근 세균잎마름병은 세계적으로 가장 중요한 병에 속한다. 지난 10년 전 국내에서 처음 발견됨으로써 당근 생산에 큰 위협이 되는 병으로 간주되었다. 원인균으로 Xathomonas hortorum pv. carotae (Xhc)이며 본 연구는 제주도 당근재배지 토양에서 이 병원균을 분리 동정하고자 수행하였다. 분리한 세균 중 인공배지에서 균총의 형태가 당근 세균잎마름병균과 유사한 세균균총을 분리하여 선발하였다. 또한 이들 선발된 세균들을 대상으로 DNA 염기 서열과 NCBI 자료를 통해 몇몇 분리 세균이 Xhc으로 동정되었다. 게다가 이들 세균을 당근 잎에 접종하였더니 당근 세균잎마름병의 전형적인 병징이 나타났다. 본 연구 결과는 제주도 토양내 Xhc의 존재 가능성을 시사하고 앞으로의 당근 세균잎마름병 방제 수단을 강구하는 데 매우 필요한 자료로서 가치가 있다고 본다.

Keywords

Acknowledgement

This work was carried out with the support of "Cooperative Research Program for Agriculture Science & Technology Development (PJ0162412021)" Rural Development Administration, Republic of Korea.

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