식물병연구 (Research in Plant Disease)

한국식물병리학회 (The Korean Society of Plant Pathology)
권호 표지
DOI QR코드

DOI QR Code

Septobasidium sp.에 의한 구실잣밤나무 고약병의 분자학적 다양성 분석

Analysis of Molecular Diversity in Castanopsis sieboldii with Felt Disease Caused by Septobasidium sp.

  • 이건우 (전북대학교 산림환경과학과) ;
  • 서상태 (국립산림과학원 산림병해충연구과) ;
  • 차병진 (충북대학교 식물의학과) ;
  • 한상섭 (전북대학교 산림환경과학과)
  • Geon-Woo Lee (Department of Forest Environment Science, Jeonbuk National University) ;
  • Sang-Tae Seo (Forest Entomology and Pathology Division, National Institute of Forest Science) ;
  • Byeongjin Cha (Department of Plant Medicine, Chungbuk National University) ;
  • Sang-Sub Han (Department of Forest Environment Science, Jeonbuk National University)
  • 투고 : 2023.12.04
  • 심사 : 2023.12.12
  • 발행 : 2023.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

2020년 제주도 동백동산 내 구실잣밤나무 수피에 고약병과 관련된 Septobasidium sp.가 발견되었다. 분리한 균주는 습실 처리하여 새로 생성된 균사에 대한 genomic DNA를 추출한 뒤 internal transcribed spacer 및 small subunit rDNA 유전자에 대해 염기서열을 밝혔으며 polymerase chain reaction-based restriction fragment length polymorphism 분석을 통해 균주들간의 분자학적 특성이 조사되었다. 이 새로운 Septobasidium sp.은 기존에 알려진 고약병들과 형태학적 및 계통학적으로 다르게 나타나 새로운 종으로 보고한다. 또한 이 고약병은 관찰 당시 주변의 다른 수목들에서는 발생하지 않고 오직 구실잣밤 나무에서만 나타나는 특성으로 기주특이성이 매우 높다는 것이 확인되었다.

In 2020, within the Dongbaekdongsan area in Jeju Island, a Septobasidium sp. associated with a felt disease in Castanopsis sieboldii (Makino) Hatus. ex T. Yamaz. & Mashiba was identified. The symptom included the presence of brown, thin, and silk-like mycelial mats attached to the tree's bark, displaying variations in size from large to small. To induce hyphal growth, the samples collected were incubated in a moist chamber, and the newly formed hyphae were subjected to genomic DNA extractions. The nucleotide sequences of the internal transcribed spacer and small subunit rDNA genes were determined, and molecular characteristics among the isolates were investigated through polymerase chain reaction-based restriction fragment length polymorphism analysis. This Septobasidium sp. exhibited distinct morphological and phylogenetic features compared to those that were previously reported in South Korea. Consequently, this strain is taxonomically classified as a provisionally novel species of Septobasidium. Furthermore, the observed felt disease exhibited a high degree of host specificity, as it was exclusively identified in C. sieboldii without occurrence in other tree species at the time of observation.

키워드

과제정보

This work was supported by the research grant of the Chungbuk National University.

참고문헌

  1. Choi, I.-Y., Lee, W.-H., Lee, J.-J., Park, M.-J., Ko, J.-A., Choi, J.-R. et al. 2016. Characterization of a Septobasidium sp. associated with felt disease of Schisandra chinensis. Mycobiology 44: 58-62. https://doi.org/10.5941/MYCO.2016.44.1.58
  2. Couch, J. N. 1938. The genus Septobasidium. University of North Carolina Press, Chapel Hill, NC, USA. 480
  3. Gomez, L. D. and Henk, D. A. 2004. Validation of the species of Septobasidium (Basidiomycetes) described by John N. Couch. Lankesteriana 4: 75-96. https://doi.org/10.15517/lank.v4i1.22985
  4. Ha, J.-S., Song, I.-K., Kim, S.-H. and Kim, J.-W. 2021. Brown felt on persimmon (Diospyros kaki) caused by Septobasidium sp. in Korea. Res. Plant Dis. 27: 66-69. https://doi.org/10.5423/RPD.2021.27.2.66
  5. Henk, D. A. 2005. New species of Septobasidium from southern Costa Rica and the southeastern United States. Mycologia 97: 908-913. https://doi.org/10.3852/mycologia.97.4.908
  6. Henk, D. A. and Vilgalys, R. 2007. Molecular phylogeny suggests a single origin of insect symbiosis in the Pucciniomycetes with support for some relationships within the genus Septobasidium. Am. J. Bot. 94: 1515-1526. https://doi.org/10.3732/ajb.94.9.1515
  7. Korean Society of Plant Pathology. 2022. List of Plant Diseases in Korea. Korean Society of Plant Pathology, Seoul, Korea. 630 pp. (In Korean)
  8. Kumar, T. K. A., Celio, G. J., Matheny, P. B., McLaughlin, D. J., Hibbett, D. S. and Manimohan, P. 2007. Phylogenetic relationships of Auriculoscypha based on ultrastructural and molecular studies. Mycol. Res. 111: 268-274. https://doi.org/10.1016/j.mycres.2006.12.003
  9. Lee, J.-C., Lee, B.-D., Lee, H.-Y., Chung, D.-O. and Eun, J.-B. 2009. Nutritional compositions in edible portion of Castanopsis cuspidata seeds. J. Korean Soc. Food Sci. Nutr. 38: 649-652. (In Korean) https://doi.org/10.3746/jkfn.2009.38.5.649
  10. Lee, Y. N. 1998. Flora of Korea. 3th ed. Kyohak Publishing Co., Ltd., Seoul, Korea. p. 72. (In Korean)
  11. Lu, C. and Guo, L. 2009. Septobasidium annulatum sp. nov. Septobasidiaceae and S. kameii new to China. Mycotaxon 110: 239-245. https://doi.org/10.5248/110.239
  12. Patouillard, N. T. 1892. Septobasidium, nouveau genre d'Hymenomycetes heterobasidies. J. Bot. 6: 61-64.
  13. Xing, X., Jacquemyn, H., Gai, X., Gao, Y., Liu, Q., Zhao, Z. et al. 2019. The impact of life form on the architecture of orchid mycorrhizal networks in tropical forest. Oikos 128: 1254-1264. https://doi.org/10.1111/oik.06363
  14. White, T. J., Bruns, T., Lee, S. and Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: A Guide to Methods and Applications, eds. by M. A. Innis, D. H. Gelfand, J. J. Sninsky and T. J. White, pp. 315-322. Academic Press, New York, NY, USA.