DOI QR코드

DOI QR Code

Acetic-acid로 대장염을 유발한 생쥐에 투여한 만년환의 치료효과

Effects of Mannyeon-hwan on Acetic Acid-induced Colitis in Rats

  • 공원호 (동의대학교 한의과대학 비계내과학교실) ;
  • 김범회 (동의대학교 한의과대학 해부학교실) ;
  • 김원일 (동의대학교 한의과대학 비계내과학교실)
  • Won-ho Kong (Dept. of Korean Internal Medicine, College of Korean Medicine, Dong-Eui University) ;
  • Bum-hoi Kim (Dept. of Anatomy, College of Korean Medicine, Dong-Eui University) ;
  • Won-ill Kim (Dept. of Korean Internal Medicine, College of Korean Medicine, Dong-Eui University)
  • 투고 : 2023.11.03
  • 심사 : 2023.12.29
  • 발행 : 2023.12.30

초록

Objectives: This study is designed to verify the effects of Mannyeon-hwan (MNH) on acetic acid-induced colitis in rats. Methods: Colitis was induced in male Sprague-Dawley rats weighing approximately 250 g by injecting acetic acid through the anus. The rats were classified into four groups: normal group, acetic acid (AA) group, AA+MNH (L) (low concentration) group, and AA+MNH (H) (high concentration) group. The body weight, visual evaluation of the colonic mucosa, anatomical histological changes, and changes in the expression of cytokines in the colon tissue were compared and analyzed. Results: Compared with the normal group, weight loss was observed in mice induced with colitis. Compared with the AA group, weight loss recovery occurred in the AA+MNH (L) and AA+MNH (H) groups, and significant changes were observed after the sixth day. In the visual evaluation of the colonic mucosa, a significant decrease in damage indicators was observed in the AA+MNH (L) and AA+MNH (H) groups compared with the AA group. In terms of anatomical histological changes and changes in the expression of tumor necrosis factor-α and interleukin-6 in colon tissue, a significant decrease was observed in the AA+MNH (L) and AA+MNH (H) groups compared with the AA group. A more pronounced decrease was observed in the AA+MNH (H) group compared with the AA+MNH (L) group. Conclusion: The effects of MNH on colitis were confirmed through research. MNH can be used as a first-line treatment for patients complaining of colitis who visit oriental medicine clinics.

키워드

과제정보

이 논문은 2023학년도 동의대학교 교내연구비에 의해 연구되었음(202300640001).

참고문헌

  1. YAZAKI Y0. Asakura Internal medicine. Seoul: Woori Medical Books; 2022, p. 96-114.
  2. Kasper D, Fauci A, Hauser S, Longo D, Jameson J, et al. Harrison's principles of internal medicine 19e. New York: MIP; 2015.
  3. Choi CH, Moon W, Kim YS, Kim ES, Lee BI, Jung YH, et al. Second Korean Guideline for the Management of Ulcerative Colitis. Korean J Gastoenterol 2017;69(1):1-28. https://doi.org/10.4166/kjg.2017.69.1.1
  4. Park JJ, Yang SK, Ye BD, Kim JW, Park DI, Yoon H, et al. Second Korean Guidelines for the Management of Crohn's Disease. Korean J Gastoenterol 2017;69(1):29-59. https://doi.org/10.4166/kjg.2017.69.1.29
  5. Department of gastrointestinal medicine Korean medicine. Gastrointestinal internal medicine. Seoul: Gunja; 2008, p. 366-96.
  6. Seo CH, Lee HH, Choi KS, Lim DS, Ryu HC, Lee YS, et al. Two Cases of Ulcerative Colitis Diagnosed as Damp-Heat Dysentery Treated with Jakyaktang-gamibang. J Int Korean Med 2006;27(4):984-90.
  7. Kim SH, Kim HJ, Kim JE, Lee SH, Hong SH, Kim WI. Clinical Case of Symptoms Remaining after Western Medical Therapy in Ulcerative Colitis, with Herbal Medicine Dansamboheol-tang gagam, Acupuncture, and Moxibustion Treatment. J Int Korean Med 2007;28(4):911-8.
  8. Hwang JW, Choi HJ, Baik YS, Jeong SH, Shin GC, Lee WC. A Case of Pyungwijiyutang-gamibang Diagnosed as Constipation due to Stagnation of Eum with Ulcerative Colitis. J Int Korean Med 2007;28(4):972-7.
  9. Choi HJ, Kim YH, Baik YS, Ma MJ, Choi IY. A Case of Ulcerative Colitis Diagnosed as Dual deficiency of the spleen-kidney Treated with Chojunggunbiwhan-gamibang. J Int Korean Med 2008;29(1):251-6.
  10. Kim EY, Seo JW, Yang EJ, Woo YJ, Song BY, Ok JY, et al. A Case of Ischemic Colitis Treated with Boolwhangumjeonggi-san. J Int Korean Med 2011;fal:465-72.
  11. Ko HN, Kim LH, Rhee YJ, Lee SH, Sun YC, Kim KH, et al. A Case of Ulcerative Colitis Diagnosed as 'Damp-Heat' Treated with Hwangryunhaedoktang Extract Granule. J Int Korean Med 2012;fal:273-9.
  12. Choi SW, Oh JS, Kim YS. A Case of Ulcerative Colitis Treated with Goihwasan and Gami-Jeonssibackchulsan. J Int Korean Med 2013;spr:157-63.
  13. Park SG, Kim GY, Shin SM, Ko H. Constitution Therapy of Adolescent Ulcerative Colitis: A Case Report. J Int Korean Med 2017;38(4):531-40. https://doi.org/10.22246/jikm.2017.38.4.531
  14. Heo G, Jang MW, Lim SW. The Effects of Coicis Semen Extract (CSE) on Dextran Sulfate Sodium - Induced Colitis in Mice. J Int Korean Med 2012;33(4):520-32.
  15. Kim SY, Park JW, Ryu BH. Effects of Auklandia Lappa on Dextran Sulfate Sodium-Induced Murine Colitis. J Int Korean Med 2013;34(2):134-46.
  16. Kim ES, Shin MK, Kim TR, Shin MH, Lee YS. Effect of Ohmae-hwan and Mume Fructus on DSS-Induced Inflammatory Bowel Disease in a Mice Model System. J Int Korean Med 2015;36(3):284-96.
  17. Song YG, Ryu BH, Yoon SW. The Effects of Soyumjungjang-tang on DSS-Induced Ulcerative Colitis in Mouse. J Int Korean Med 2008;29(2):385-400.
  18. Lee JA, Kong KH. Effects of Doche-tang on Colonic Mucosal Ulcer Induced by DSS in Mice. J Int Korean Med 2008;29(3):752-64.
  19. Bae KH, Kong KH. Effects of Goihwa-san on Ulcerative Colitis Induced by Dextran Sulfate Sodium in mice. J Int Korean Med 2010;31(3):513-25.
  20. Choi JY, Ahn SH, Shih YH, Sa BS, Kim KB. Mitigating Effect of Jageum-jung on Dextran Sulfate Sodium-induced Ulcerative Colitis through Anti-inflammatory Regulation. J Int Korean Med 2017;38(6):944-54. https://doi.org/10.22246/jikm.2017.38.6.944
  21. Kang A, Lim SW. Therapeutic Effects of Gaejigayonggolmoryo-tang on Dextran Sodium Sulfate-induced Ulcerative Colitis in Mice. J Int Korean Med 2017;38(6):1021-34. https://doi.org/10.22246/jikm.2017.38.6.1021
  22. Kim YK, Moon YH. A Study of the Effectiveness of Hwanggeum-tang and Gamchosasim-tang in the Mice Model of Inflammatory Bowel Disease. J Int Korean Med 2021;42(3):351-74. https://doi.org/10.22246/jikm.2021.42.3.351
  23. Bae WS. Hanbangimsanghak. Seoul: Namsandang; 1976, p. 72.
  24. Axelrad JE, Lichtiger S, Yajnik V. Inflammatory bowel disease and cancer: The role of inflammation, immunosuppression, and cancer treatment. World J Gastroenterol 2016;22(20):4794-801. https://doi.org/10.3748/wjg.v22.i20.4794
  25. Owusu G, Obiri DD, Ainooson GK, Osafo N, Antwi AO, Duduyemi BM, et al. Acetic Acid-Induced Ulcerative Colitis in Sprague Dawley Rats is Suppressed by Hydroethanolic Extract of Cordia vignei Leaves through Reduced Serum Levels of TNF-α and IL-6. Int J Chronic Dis 2020;2020:8785497.
  26. Hunschede S, Kubant R, Akilen R, Thomas S, Anderson GH. Decreased Appetite after High-Intensity Exercise Correlates with Increased Plasma Interleukin-6 in Normal-Weight and Overweight/Obese Boys. Curr Dev Nutr 2017;1(3):e000398.
  27. Choi EY, Cho KK, Choi IS. Inflammatory Bowel Disease and Cytokine. Journal of Life Science 2013;23(3):448-61. https://doi.org/10.5352/JLS.2013.23.3.448
  28. Strober W, Fuss IJ. Proinflammatory cytokines in the pathogenesis of inflammatory bowel diseases. Gastroenterology 2011;140(6):1756-67. https://doi.org/10.1053/j.gastro.2011.02.016
  29. An editorial department of Jungdam. Steps to Internal Medicine Vol. 7. Imunology/Allergy. Seoul: Jungdam; 2013, p. 3-26.
  30. Jang BI. Animal Models of Inflammatory Bowel Disease. Intestinal research (Intest Res) 2008;6(1):8-18.
  31. Chen Y, Si JM, Liu WL, Cai JT, Du Q, Wang LJ, et al. Induction of experimental acute ulcerative colitis in rats by administration of dextran sulfate sodium at low concentration followed by intracolonic administration of 30% ethanol. J Zhejiang Univ Sci B 2007;8(9):632-7. https://doi.org/10.1631/jzus.2007.B0632