Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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Validation of Synovial Fluid Clinical Samples for Molecular Detection of Pathogens Causing Prosthetic Joint Infection Using GAPDH Housekeeping Gene as Internal Control

  • Jiyoung Lee (Department of Research & Development, DreamDX) ;
  • Eunyoung Baek (Department of Research & Development, DreamDX) ;
  • Hyesun Ahn (Joint & Arthritis Research, Himchan Hospital) ;
  • Youngnam Park (Department of Dental Hygiene, Gimcheon University) ;
  • Geehyuk Kim (Division of Risk Assessment, Bureau of Public Health Emergency Preparedness, Korea Disease Control and Prevention Agency) ;
  • Sua Lim (Department of Research & Development, DreamDX) ;
  • Suchan Lee (Joint & Arthritis Research, Himchan Hospital) ;
  • Sunghyun Kim (Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan)
  • Received : 2023.10.25
  • Accepted : 2023.11.15
  • Published : 2023.12.31
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Abstract

Identification of the pathogens causing infection is important in terms of patient's health management and infection control. Synovial fluids could be used as clinical samples to detect causative pathogens of prosthetic joint infections (PJIs) using molecular diagnostic assays, therefore, normalization and validation of clinical samples are necessary. Microbial culture is considered the gold standard for all infections, including PJIs. Recently, molecular diagnostic methods have been developed to overcome the limitation of microbial culture. Therefore, guideline for validating clinical samples to provide reliable results of molecular diagnostic assays for infectious diseases is required in clinical field. The present study aimed to develop an accurate validating method of synovial fluid clinical samples using GAPDH gene as an internal control to perform the quantitative PCR TaqMan probe assay to detect pathogens causing PJIs.

Keywords

Acknowledgement

This work was supported by Busan Techno Park by the Korea government (Busan).

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