Korean Journal of Ecology and Environment (생태와환경)

The Korean Society of Limnology (한국수생태학회)
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Bioaccumulation and Expressions of Stress Response Genes in Benthic Oligochaete Worm Tubifex tubifex to Exposure of Cadmium-spiked Sediment

카드뮴 (Cd) 노출 퇴적물에 따른 실지렁이 Tubifex tubifex의 체내 축적과 스트레스 반응 유전자 발현

  • Ji-Hoon Kim (Department of Ocean Integrated Science, Chonnam National University) ;
  • Won-Seok Kim (Department of Ocean Integrated Science, Chonnam National University) ;
  • Kiyun Park (Fisheries Science Institute, Chonnam National University) ;
  • Ihn-Sil Kwak (Department of Ocean Integrated Science, Chonnam National University)
  • 김지훈 (전남대학교 해양융합과학과) ;
  • 김원석 (전남대학교 해양융합과학과) ;
  • 박기연 (전남대학교 수산과학연구소) ;
  • 곽인실 (전남대학교 해양융합과학과)
  • Received : 2023.08.08
  • Accepted : 2023.12.03
  • Published : 2023.12.31
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Abstract

Cadmium (Cd), a heavy metal found in the aquatic environment, accumulates in organisms through the food chain. In the study, we investigated the survival rates, measurement of body Cd levels, and expression analysis of the stress response genes (Heat shock protein 70: HSP70 and Heat shock protein 60: HSP60) and antioxidant enzyme Glutathione S-Transferases (GST) on benthic oligochaete worm Tubifex tubifex exposed three concentrations of Cd, to analyze the bioaccumulation and changes of stress gene expressions to exposure toxicity of the Cd-spiked sediment. Survival rates of T. tubifex exposed to the Cdspiked sediment were 93% at 0.4 mg kg-1 Cd, 96% at 1.87 mg kg-1 Cd, and 93% at 6.09 mg kg-1 Cd for 10 days. Cd concentration in the body of T. tubifex was higher than that in the sediment. After Cd exposures for 10 days, the body Cd levels were 18.4 mg kg-1, 13.06 mg kg-1, and 79.11 mg kg-1 at exposed three concentrations of Cd, respectively. Upregulation of HSP70 gene expression was observed at all concentrations of exposed Cd as a time-dependent manner, whereas transcriptional expression of the HSP60 gene increased as a timedependent manner in T. tubifex exposed to the relative high concentration (6.09 mg kg-1) of Cd. However, GST gene expression increased on day 1 at all concentrations after Cd exposures, and then downregulated until 10 days. These results indicate to ecotoxicological and molecular effects in benthic oligochaete worm T. tubifex to Cd-spiked sediment and provide the basic information for the utilization of environmental toxicity assessment using the T. tubifex as a aquatic pollution indicator species.

환경에 존재하는 중금속인 Cd은 생물에게 유입되며 먹이사슬을 통해 생물에게 축적된다. 본 연구에서는 카드뮴 노출에 따른 오염지표생물인 실지렁이(Tubifex tubifex)의 체내 농축 반응 및 스트레스 유전자 발현 패턴을 분석하기 위해, 퇴적물 내 카드뮴 노출 후 생존율 변화, 체내 농축 농도 측정, 그리고 스트레스 반응 유전자인 열 충격 단백질(HSP60 and HSP70)과 항산화효소인 GST의 발현 패턴을 관찰하였다, Cd 노출 퇴적물에 노출된 T. tubifex의 생존율은 노출 10일에 0.4 mg kg-1 Cd에서 93%, 1.87 mg kg-1 Cd에서 96%, 6.09 mg kg-1 Cd에서 93%로 관찰되었다. T. tubifex의 체내 Cd 농도는 퇴적물 내 Cd 농도에 비해 높게 나타났으며, 0.4 mg kg-1 Cd에서는 실험 10일에 18.4 mg kg-1 Cd, 1.87 mg kg-1 Cd에서 실험 1일에 13.06 mg kg-1 Cd, 6.09 mg kg-1 Cd에서 실험 10일에 79.11 mg kg-1 Cd로 가장 높게 나타났다. HSP60 유전자 발현은 6.09 mg kg-1 Cd 노출 퇴적물에서 시간 의존적으로 발현이 대조군에 비해 증가였으며, HSP70은 모든 농도에서 대조군에 비해 시간 의존적인 발현의 증가를 보였다. 또한, GST는 모든 농도에서 실험 1일에 대조군에 비해 발현이 증가한 후, 실험 10일까지 발현이 감소하였다. 이러한 연구결과는 퇴적물 내에 존재하는 Cd에 대한 실지렁이의 생태독성학적 및 분자유전학적 독성영향을 확인한 결과로, 환경 내 오염지표종으로 실지렁이를 이용한 환경독성평가의 기초자료로 활용될 수 있을 것이다.

Keywords

Acknowledgement

이 논문은 한국연구재단의 지원 (NRF-2018-R1A6A1A-03024314, NRF-2020-R1A2C1013936)과 환경부의 재원으로 한국환경산업기술원 수생태계 건강성 확보 기술개발사업의 지원(2021003050001)을 받아 수행된 연구임.

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