Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Green tea extract addition into a Tris-based egg yolk extender improves Bali bull sperm quality

  • Ragil Angga, Prastiya (Veterinary Reproduction Division, School of Health and Life Sciences (SIKIA), Universitas Airlangga) ;
  • Tri Wahyu, Suprayogi (Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga) ;
  • Aldea Erian, Debora (Veterinary Reproduction Division, School of Health and Life Sciences (SIKIA), Universitas Airlangga) ;
  • Ani, Wijayanti (Veterinary Reproduction Division, School of Health and Life Sciences (SIKIA), Universitas Airlangga) ;
  • Anny, Amalia (Singosari National Artificial Insemination Center, Directorate General of Livestock and Animal Health) ;
  • Deny, Sulistyowati (Singosari National Artificial Insemination Center, Directorate General of Livestock and Animal Health) ;
  • Aras Prasetiyo, Nugroho (Animal Husbandry Science Department, Faculty of Animal Husbandry, Jenderal Soedirman University)
  • Received : 2022.05.05
  • Accepted : 2022.08.06
  • Published : 2023.02.01
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Abstract

Objective: The conservation of Bali bulls, the Indonesian native breed of cattle, is crucial for cattle breeding in Indonesia. To guarantee the spread of Bali bulls through artificial insemination the quality of the frozen semen must be high. To this end, adding an extender material to semen that increases spermatozoa's survival during cryopreservation is important. Green tea extract (GTE) can be used as cryoprotectant because its high antioxidant activity can help avoid reactive oxygen species formation. Methods: Semen of five Bali bulls from the National Artificial Insemination Center at Singosari, Indonesia was collected routinely twice a week. First, fresh semen inspection was performed to determine the feasibility of using Bali bulls as animal samples. The extender used in this study was Tris-based egg yolk. The samples were divided into four treatments: T0, no GTE added to the extender; T1, 0.05 mg GTE plus 100 mL extender; T2, 0.10 mg GTE plus 100 mL extender; and T3, 0.15 mg GTE plus 100 mL extender. The semen freezing process was conducted according to standard procedures and sperm quality parameters, i.e., sperm motility, viability, abnormalities, and membrane integrity observed pre-freezing and post-thawing. Results: There were significant differences in total motility, progressive motility, viability, and integrity membrane of Bali bull sperm at both pre-freezing and post-thawing after adding GTE into the extender. In contrast, there were no differences in abnormalities among treatments. Conclusion: Adding GTE at a 0.15 mg into 100 mL Tris-based egg yolk extender can improve the quality of cryopreserved Bali bull sperm.

Keywords

Acknowledgement

The authors would like to thank the National Artificial Insemination Center (NAIC) at Singosari, Malang, East Java, Indonesia for providing the necessary data and accompaniment for this study. The authors also thank the Universitas Airlangga for the final project recognition funding (66/UN3.15/PT/2022-2023).

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