대한수의학회지 (Korean Journal of Veterinary Research)

  • 제63권4호
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  • Pages.40.1-40.7
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  • 2023
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  • 2466-1384(pISSN)
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  • 2466-1392(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
권호 표지
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Follicular fluid-derived extracellular vesicles improve in vitro maturation and embryonic development of porcine oocytes

  • Heejae Kang (Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University) ;
  • Seonggyu Bang (Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University) ;
  • Heyyoung Kim (Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University) ;
  • Ayeong Han (Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University) ;
  • Shuntaro Miura (Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University) ;
  • Hye Sun Park (Korea Basic Science Institute) ;
  • Islam M. Saadeldin (Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University) ;
  • Sanghoon Lee (Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University) ;
  • Jongki Cho (Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University)
  • 투고 : 2023.10.11
  • 심사 : 2023.12.03
  • 발행 : 2023.12.31
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초록

To optimize the most efficient method for porcine in vitro maturation (IVM), we compared the effects of supplementing extracellular vesicles (EVs) derived from porcine follicular fluid (pFF). The cumulus oocyte complexes were grouped into 4 groups with different supplementations as following: pFF (G1), pFF-depleted EVs (G2), EVs (G3) and control (G4) groups. After IVM with different supplementations, maturation rates and the developmental competences of porcine oocytes and blastocyst development were investigated. Additionally, glutathione (GSH) and reactive oxygen species (ROS) levels were measured in mature oocytes. The EVs were isolated and characterized with cryo-TEM and nanoparticle tracking analysis. The pFF significantly affected the maturation rate, whereas the presence of EVs did not show notable difference in the maturation rates. Although there were numerical increases in the measured parameters in EV and pFF-depleted EVs groups, no significant differences were observed between them. The EV group showed similar oocyte maturation rate for both positive and negative control groups. The GSH was not different among the groups, but ROS levels were significantly lower in pFF-supplemented group when compared with other groups with the highest level in the control group. G2 group wasn't significantly different G1 and G3 group. G3 group wasn't significantly different from G2 and G4 group. This suggests that EVs in IVM medium which probably effected partially to protect against oxidative stress and potentially enhance the quality of oocytes. This study indicates that the EVs in pFF play a significant role in improving the efficiency of oocyte maturation in porcine.

키워드

과제정보

This study was supported by the Research Fund of the Chungnam National University. We would like to thank Mrs. Seongja Kim and Daejeon slaughterhouse for providing pig ovaries.

참고문헌

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