Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Prevalence of antibody and toxin against edema disease from pig farms in Jeonbuk province

전북지역 양돈장에서의 돼지 부종병 항체 및 톡신 양성률 조사

  • Sun-Young Cho (Jeollabuko Veterinary Service Laboratory West Branch) ;
  • Jeong Hee Yu (Department of Veterinary Public Health, College of Veterinary Medicine, Jeonbuk National University) ;
  • Yeong Ju Yu (Department of Veterinary Public Health, College of Veterinary Medicine, Jeonbuk National University) ;
  • Han-Jun Lee (Jeollabuko Veterinary Service Laboratory West Branch) ;
  • Jin Hur (Department of Veterinary Public Health, College of Veterinary Medicine, Jeonbuk National University)
  • 조선영 (전라북도 동물위생시험소 서부지소) ;
  • 유정희 (전북대학교 수의과대학 수의공중보건학실) ;
  • 유영주 (전북대학교 수의과대학 수의공중보건학실) ;
  • 이한준 (전라북도 동물위생시험소 서부지소) ;
  • 허진 (전북대학교 수의과대학 수의공중보건학실)
  • Received : 2023.12.10
  • Accepted : 2023.12.12
  • Published : 2023.12.30
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Abstract

Edema disease (ED) in pigs is enterotoxemia caused by Shiga toxin type 2e (Stx2e)-producing Escherichia coli (STEC) and frequently occurs in young piglets. Therefore, ED causes enormous economic losses in pig farms. In this study, a modified Stx2e (mStx2e) antigen was expressed and purified using commercial E. coli expression system. Monoclonal antibody was serviced by Ynto Ab Inc., using Phage Display Technique. Anti-Stx2e antibodies in piglets were measured by indirect ELISA using mStx2e antigens. Naive Stx2e in piglets were detected by Sandwich ELISA using Stx2e-monoclonal antibodies and commercial Stx2e-polyclonal antibodies. Among 3,480 piglets, anti-Stx2e antibodies were observed in 2,573 piglets. The 49.4% among 830 piglet serum samples possessed 0.625 ㎍/mL or more of Stx2e proteins. The 18.3% of 830 sera had 0.313 ㎍/mL of Stx2e proteins. The 32.3% of 830 samples held 0.156 ㎍/mL or less of Stx2e proteins. These results show that indirect ELISA using mStx2e antigen and Sandwich ELISA using Stx2e-monoclonal and polyclonal antibodies can be useful to detect ED in piglets.

Keywords

Acknowledgement

이 연구는 한국정부(MSIT)가 설립한 한국연구재단(NRF)의 교육부 학문균형발전지원사업(보호연구지원사업) 연구비의 지원을 받았습니다(No.2020R1I1A2074647). 더불어 전라북도동물위생시험소 서부지소로부터 지원을 받아 연구가 수행되었습니다.

References

  1. Acheson DW, Jacewicz M, Kane AV, Donohue-Rolfe A, Keusch GT. 1993. One step high yield affinity purification of shiga-like toxin II variants and quantitation using enzyme linked immunosorbent assays. Microb Pathog 14: 57-66. https://doi.org/10.1006/mpat.1993.1006
  2. Aghebati-Maleki L, Bakhshinejad B, Baradaran B, Motallebnezhad M, Aghebati-Maleki A, Nickho H, Yousefi M, Majidi J. 2016. Phage display as a promising approach for vaccine development. J Biomed Sci 23: 66.
  3. Ahmed W, Gyawali P, Toze S. 2015. Quantitative PCR measurements of Escherichia coli including shiga toxin-producing E. coli (STEC) in animal feces and environmental waters. Environmental science & technology 49: 3084-3090. https://doi.org/10.1021/es505477n
  4. Baldo V, Salogni C, Giovannini S, D'Incau M, Boniotti MB, Birbes L, Alborali GL. 2020. Pathogenicity of Shiga toxin type 2e Escherichia coli in pig colibacillosis. Front Vet Sci 7: 545818.
  5. Barbau-Piednoir E, Denayer S, Botteldoorn N, Dierick K, De Keersmaecker SC, Roosens NH. 2018. Detection and discrimination of five E. coli pathotypes using a combinatory SYBR® Green qPCR screening system. Appl Microbiol Biotechnol 102: 3267-3285. https://doi.org/10.1007/s00253-018-8820-0
  6. Bazan J, Calkosinski I, Gamian A. 2012. Phage display-A powerful technique for immunotherapy: 1. Introduction and potential of therapeutic applications. Human Vaccin Immunother 8: 1817-1828. https://doi.org/10.4161/hv.21703
  7. Berger PI, Hermanns S, Kerner K, Schmelz F, Schuler V, Ewers C, Bauerfeind R, Doherr MG. 2023. Cross-sectional study: prevalence of oedema disease Escherichia coli (EDEC) in weaned piglets in Germany at pen and farm levels. Porcine Health Manag 9: 49.
  8. Bettelheim KA, Beutin L. 2003. Rapid laboratory identification and characterization of verocytotoxigenic (Shiga toxin producing) Escherichia coli (VTEC/STEC). J Appl Microbiol 95: 205-217. https://doi.org/10.1046/j.1365-2672.2003.02031.x
  9. Boerlin P, McEwen SA, Boerlin-Petzold F, Wilson JB, Johnson RP, Gyles CL. 1999. Associations between virulence factors of Shiga toxin-producing Escherichia coli and disease in humans. J Clin Microbiol 37: 497-503. https://doi.org/10.1128/JCM.37.3.497-503.1999
  10. Bosworth BT, Samuel JE, Moon HW, O'brien AD, Gordon VM, Whipp SC 1996. Vaccination with genetically modified Shiga-like toxin IIe prevents edema disease in swine. Infec immun 64: 55-60. https://doi.org/10.1128/iai.64.1.55-60.1996
  11. Butler JE, Lager KM, Splichal I, Francis D, Kacskovics I, Sinkora M, Ramsoondar J. 2009. The piglet as a model for B cell and immune system development. Vet Immunol Immunopathol 128: 147-170. https://doi.org/10.1016/j.vetimm.2008.10.321
  12. Casanova NA, Redondo LM, Dailoff GC, Arenas D, Miyakawa MEF. 2018. Overview of the role of Shiga toxins in porcine edema disease pathogenesis. Toxicon 148: 149-154. https://doi.org/10.1016/j.toxicon.2018.04.019
  13. Fan B, Sun J, Zhu L, Zhou J, Zhao Y, Yu Z, Li B. 2020. Development of a novel double antibody sandwich quantitative enzyme-linked immunosorbent assay for detection of porcine epidemic diarrhea virus antigen. Front Vet Sci 7: 540248.
  14. Farzaneh M, Derakhshandeh A, Al-Farha AABA, Petrovski, K, Hemmatzadeh F. 2022. A novel phagedisplayed MilA ELISA for detection of antibodies against Myc. bovis in bovine milk. J Appl Microbiol 133: 1496-1505. https://doi.org/10.1111/jam.15655
  15. Feng PC, Jinneman K, Scheutz F, Monday SR. 2011. Specificity of PCR and serological assays in the detection of Escherichia coli Shiga toxin subtypes. Appl Environ Microbiol 77: 6699-6702. https://doi.org/10.1128/AEM.00370-11
  16. Gehring AG, Fratamico PM, Lee J, Ruth LE, He X, He Y, Rubio FM. 2017. Evaluation of ELISA tests specific for Shiga toxin 1 and 2 in food and water samples. Food Cont 77: 145-149. https://doi.org/10.1016/j.foodcont.2017.02.003
  17. Gordon VM, Whipp SC, Moon HW, O'Brien AD, Samuel JE. 1992. An enzymatic mutant of Shiga-like toxin II variant is a vaccine candidate for edema disease of swine. Infect Immun 60: 485-490. https://doi.org/10.1128/iai.60.2.485-490.1992
  18. Imberechts H, Deprez P, Van Driessche E, Pohl P. 1997. Chicken egg yolk antibodies against F18ab fimbriae of Escherichia coli inhibit shedding of F18 positive E. coli by experimentally infected pigs. Vet Microbiol 54: 329-341. https://doi.org/10.1016/S0378-1135(96)01293-X
  19. Johannes L, Romer W. 2010. Shiga toxins-from cell biology to biomedical applications. Nat Rev Microbiol 8: 105-116. https://doi.org/10.1038/nrmicro2279
  20. Johansen M, Andresen LO, Jorsal SE, Thomsen LK, Waddell TE, Gyles CL. 1997. Prevention of edema disease in pigs by vaccination with verotoxin 2e toxoid. Can J Vet Res 61: 280.
  21. Joseph A, Cointe A, Kurkdjian PM, Rafat C, Hertig A. 2020. Shiga Toxin-Associated Hemolytic Uremic Syndrome: A Narrative Review. Toxins (Basel) 12: 67.
  22. Kim MJ, Kim SH, Kim TS, Kee HY, Seo JJ, Kim E S, Lee J. 2009. Identification of Shiga toxin-producing E. coli isolated from diarrhea patients and cattle in Gwangju area, Korea. J Bacteriol Virol 39: 29-39. https://doi.org/10.4167/jbv.2009.39.1.29
  23. MacLeod DL, Gyles CL. 1990 Purification and characterization of an Escherichia coli Shiga-like toxin II variant. Infect Immun 58: 1232-1239. https://doi.org/10.1128/iai.58.5.1232-1239.1990
  24. Meng Q, Bai X, Zhao A, Lan R, Du H, Wang T, Xiong Y. 2014. Characterization of Shiga toxin-producing Escherichia coli isolated from healthy pigs in China. BMC microbiol 14: 1-14. https://doi.org/10.1186/1471-2180-14-5
  25. Mesonero-Escuredo S, Morales J, Mainar-Jaime RC, Diaz G, Arnal JL, Casanovas C, Barrabes S, Segales J. 2021. Effect of edema disease vaccination on mortality and growth parameters in nursery pigs in a Shiga toxin 2e positive commercial farm. Vaccines (Basel) 9: 567.
  26. Monaghan A, Byrne B, Fanning S, Sweeney T, David McDowell D, Bolton DJ. 2011. Serotypes and Virulence Profiles of Non-O157 Shiga Toxin-Producing Escherichia coli Isolates from Bovine Farms. Appl Environ Microbiol 77: 8662-8668. https://doi.org/10.1128/AEM.06190-11
  27. Nataro JP, Kaper JB. 1998. Diarrheagenic Escherichia coli. CMR 11: 142-201. https://doi.org/10.1128/CMR.11.1.142
  28. Oanh TKN, Nguyen VK, De Greve H, Goddeeris BM. 2012. Protection of piglets against Edema disease by maternal immunization with Stx2e toxoid. Infect Immun 80: 469-473. https://doi.org/10.1128/IAI.05539-11
  29. Orth D, Grif K, Khan AB, Naim A, Dierich MP, Wurzner R. 2007. The Shiga toxin genotype rather than the amount of Shiga toxin or the cytotoxicity of Shiga toxin in vitro correlates with the appearance of the hemolytic uremic syndrome. Diagn Microbiol Infect Dis 59: 235-242. https://doi.org/10.1016/j.diagmicrobio.2007.04.013
  30. Papadopoulos KP, Isaacs R, Bilic S, Kentsch K, Huet HA, Hofmann M, Mahipal A. 2015. Unexpected hepatotoxicity in a phase I study of TAS266, a novel tetravalent agonistic Nanobody® targeting the DR5 receptor. Cancer Chemother Pharmacol 75: 887-895. https://doi.org/10.1007/s00280-015-2712-0
  31. Sato T, Matsui T, Takita E, Kadoyama Y, Makino SI, Kato KO, Hamabata T. 2013. Evaluation of recombinant forms of the shiga toxin variant Stx2eB subunit and non-toxic mutant Stx2e as vaccine candidates against porcine edema disease. J Vet Med Sci 75: 1309-1315. https://doi.org/10.1292/jvms.13-0118
  32. Seo BJ, Kim H, Cho HS, Park BY, Kim WI. 2016. Evaluation of two commercial PRRSV antibody ELISA kits with samples of known status and singleton reactors. J Vet Med Sci 78: 133-138. https://doi.org/10.1292/jvms.15-0126
  33. Seo BJ, Moon JY, Jeong WKKC, Kim S, Kim WI, Hur J. 2018. Virulence-associated Genes and Antimicrobial Resistance of Escherichia coli Isolated From Post-weaning Piglets with Diarrhea in Korea. J Bacteriol Mycol 5, 1090.
  34. Seo BJ, Yu JH, Kim SC, Yu YJ, Park BY, Park JH, Hur J. 2022. Enhanced production of natural shiga toxin by overexpressing A subunit of Stx2e in Stx2eproducing Escherichia coli isolated in South Korea. bioRxiv 2022-07.
  35. Sulong P, Anudit N, Nuanualsuwan S, Mariela S, Khantasup K. 2021. Application of phage display technology for the production of antibodies against Streptococcus suis serotype 2. PLoS One 16: e0258931.
  36. Tseng M, Fratamico PM, Bagi L, Delannoy S, Fach P, Manning SD, Funk JA. 2014. Molecular characterization of Shiga toxin-producing E. coli (STEC) from finishing swine in a longitudinal study. Appl Environ Microbiol 80: 6395-6402. https://doi.org/10.1128/AEM.01761-14
  37. Wang W, Li J, Fan B, Zhang X, Guo R, Zhao Y, Li B. 2021. Development of a novel double antibody sandwich ELISA for quantitative detection of porcine deltacoronavirus antigen. Viruses 13: 2403.
  38. Yoshimura M, Honda Y, Yonemitsu E, Takahashi R, Suenaga K, Waki T. 2023. Induction of antitoxic antibody and preventive effect against porcine edema disease by the pentameric Stx2eB subunit vaccine. Vet Res 54: 29.